LAX3_MEDTR
ID LAX3_MEDTR Reviewed; 465 AA.
AC Q9FEL6;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=Auxin transporter-like protein 3;
DE AltName: Full=AUX1-like protein 3;
DE AltName: Full=MtLAX3;
GN Name=LAX3;
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=cv. Jemalong; TISSUE=Root;
RX PubMed=11277424; DOI=10.1094/mpmi.2001.14.3.267;
RA de Billy F., Grosjean C., May S., Bennett M.J., Cullimore J.V.;
RT "Expression studies on AUX1-like genes in Medicago truncatula suggest that
RT auxin is required at two steps in early nodule development.";
RL Mol. Plant Microbe Interact. 14:267-277(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE FAMILY, AND TISSUE SPECIFICITY.
RX PubMed=15375694; DOI=10.1007/s00438-004-1057-x;
RA Schnabel E.L., Frugoli J.;
RT "The PIN and LAX families of auxin transport genes in Medicago
RT truncatula.";
RL Mol. Genet. Genomics 272:420-432(2004).
CC -!- FUNCTION: Carrier protein involved in proton-driven auxin influx.
CC Mediates the formation of auxin gradient from developing leaves (site
CC of auxin biosynthesis) to tips by contributing to the loading of auxin
CC in vascular tissues and facilitating acropetal (base to tip) auxin
CC transport within inner tissues of the root apex, and basipetal (tip to
CC base) auxin transport within outer tissues of the root apex (By
CC similarity). May be involved in lateral roots and nodules formation.
CC {ECO:0000250, ECO:0000269|PubMed:11277424}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Shoots and roots of nodulating plants. Low levels
CC in roots, nodules, stems, petioles, leaves, shoot apices and flowers.
CC {ECO:0000269|PubMed:11277424, ECO:0000269|PubMed:15375694}.
CC -!- DEVELOPMENTAL STAGE: In primary roots, mostly localized in tips and to
CC a lower extent in vasculature of older regions. In root tips, mostly
CC expressed in the central tissues of the elongating zone (developing
CC vasculature), in the starch-filled cells of the root cap and in some
CC cortical cells. During lateral root development, striking expression in
CC the proximal region of the primordium, close to the primary root
CC central cylinder, and then in elongating cells of the developing
CC vasculature and in developing root cap. During nodule formation,
CC expressed in young elongated primordium, mostly in cells close to the
CC root vasculature. In later stages, confined in small cells rich in
CC amyloplasts with small nuclei. Near the periphery of developing nodules
CC strong expression at the base that tapers off toward the apex. Not
CC expressed in mature nodules. {ECO:0000269|PubMed:11277424}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC Amino acid/auxin permease (AAAP) (TC 2.A.18.1) subfamily.
CC {ECO:0000305}.
CC -!- CAUTION: Because of the similarity in sequence, the probe used to
CC describe the developmental stages did not discriminate among the
CC various MtLAX mRNAs. {ECO:0000305}.
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DR EMBL; AJ299399; CAC12997.1; -; mRNA.
DR EMBL; AY115842; AAM55303.1; -; Genomic_DNA.
DR RefSeq; XP_003601086.1; XM_003601038.2.
DR AlphaFoldDB; Q9FEL6; -.
DR SMR; Q9FEL6; -.
DR STRING; 3880.AES71337; -.
DR EnsemblPlants; AES71337; AES71337; MTR_3g072870.
DR GeneID; 11411395; -.
DR Gramene; AES71337; AES71337; MTR_3g072870.
DR eggNOG; KOG1303; Eukaryota.
DR HOGENOM; CLU_027994_2_0_1; -.
DR OMA; IRVHETK; -.
DR OrthoDB; 509343at2759; -.
DR ExpressionAtlas; Q9FEL6; differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 2: Evidence at transcript level;
KW Amino-acid transport; Auxin signaling pathway; Cell membrane; Membrane;
KW Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..465
FT /note="Auxin transporter-like protein 3"
FT /id="PRO_0000093847"
FT TOPO_DOM 1..52
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..70
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 71..72
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 94..129
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 130..150
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 151..165
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 187..189
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 211..225
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 247..259
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..280
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 281..307
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 329..349
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 350..370
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 371..374
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 375..395
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 396..421
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 422..442
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 443..465
FT /note="Extracellular"
FT /evidence="ECO:0000255"
SQ SEQUENCE 465 AA; 52597 MW; D20E5C8AA0B20593 CRC64;
MTSEKVETVV AGNYLEMERE EEGSKSTTGK LSKFFWHGGS VYDAWFSCAS NQVAQVLLTL
PYSFSQLGML SGILFQIFYG LMGSWTAYII SVLYVEYRTR KEREKVDFRN HVIQWFEVLD
GLLGKHWRNL GLFFNCTFLL FGSVIQLIAC ASNIYYINDH LDKRTWTYIF GACCATTVFI
PSFHNYRIWS FLGLVMTTYT AWYMTIASIL HGQAEDVKHS GPTKLVLYFT GATNILYTFG
GHAVTVEIMH AMWKPQKFKM IYLIATLYVM TLTLPSAAAV YWAFGDNLLT HSNALSLLPR
TGFRDTAVIL MLIHQFITFG FACTPLYFVW EKFLGVHETK SLLKRALVRL PVVIPIWFLA
IIFPFFGPIN STVGSLLVSF TVYIIPALAH MVTFASAPAR ENAVERPPSF LGGWVGLYSV
NVFVAVWVLV VGFGLGGWAS MLNFVHQIKT FGLFAKCFQC PPHKA