LAX5_MEDTR
ID LAX5_MEDTR Reviewed; 490 AA.
AC Q8L883;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 76.
DE RecName: Full=Auxin transporter-like protein 5;
DE AltName: Full=AUX1-like protein 5;
DE AltName: Full=MtLAX5;
GN Name=LAX5;
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE FAMILY, AND TISSUE SPECIFICITY.
RX PubMed=15375694; DOI=10.1007/s00438-004-1057-x;
RA Schnabel E.L., Frugoli J.;
RT "The PIN and LAX families of auxin transport genes in Medicago
RT truncatula.";
RL Mol. Genet. Genomics 272:420-432(2004).
RN [2]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=11277424; DOI=10.1094/mpmi.2001.14.3.267;
RA de Billy F., Grosjean C., May S., Bennett M.J., Cullimore J.V.;
RT "Expression studies on AUX1-like genes in Medicago truncatula suggest that
RT auxin is required at two steps in early nodule development.";
RL Mol. Plant Microbe Interact. 14:267-277(2001).
CC -!- FUNCTION: Carrier protein involved in proton-driven auxin influx.
CC Mediates the formation of auxin gradient from developing leaves (site
CC of auxin biosynthesis) to tips by contributing to the loading of auxin
CC in vascular tissues and facilitating acropetal (base to tip) auxin
CC transport within inner tissues of the root apex, and basipetal (tip to
CC base) auxin transport within outer tissues of the root apex (By
CC similarity). May be involved in lateral roots and nodules formation.
CC {ECO:0000250, ECO:0000269|PubMed:11277424}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Shoots and roots of nodulating plants, at low
CC levels. {ECO:0000269|PubMed:11277424, ECO:0000269|PubMed:15375694}.
CC -!- DEVELOPMENTAL STAGE: In primary roots, mostly localized in tips and to
CC a lower extent in vasculature of older regions. In root tips, mostly
CC expressed in the central tissues of the elongating zone (developing
CC vasculature), in the starch-filled cells of the root cap and in some
CC cortical cells. During lateral root development, striking expression in
CC the proximal region of the primordium, close to the primary root
CC central cylinder, and then in elongating cells of the developing
CC vasculature and in developing root cap. During nodule formation,
CC expressed in young elongated primordium, mostly in cells close to the
CC root vasculature. In later stages, confined in small cells rich in
CC amyloplasts with small nuclei. Near the periphery of developing nodules
CC strong expression at the base that tapers off toward the apex. Not
CC expressed in mature nodules. {ECO:0000269|PubMed:11277424}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC Amino acid/auxin permease (AAAP) (TC 2.A.18.1) subfamily.
CC {ECO:0000305}.
CC -!- CAUTION: Because of the similarity in sequence, the probe used to
CC describe the developmental stages did not discriminate among the
CC various MtLAX mRNAs. {ECO:0000305}.
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DR EMBL; AY115845; AAM55306.1; -; Genomic_DNA.
DR RefSeq; XP_013456483.1; XM_013601029.1.
DR AlphaFoldDB; Q8L883; -.
DR SMR; Q8L883; -.
DR EnsemblPlants; KEH30514; KEH30514; MTR_4g073770.
DR GeneID; 25492791; -.
DR Gramene; KEH30514; KEH30514; MTR_4g073770.
DR HOGENOM; CLU_027994_2_0_1; -.
DR OrthoDB; 570025at2759; -.
DR ExpressionAtlas; Q8L883; differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 2: Evidence at transcript level;
KW Amino-acid transport; Auxin signaling pathway; Cell membrane; Glycoprotein;
KW Membrane; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..490
FT /note="Auxin transporter-like protein 5"
FT /id="PRO_0000093849"
FT TOPO_DOM 1..55
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 74..75
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 97..132
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 154..168
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 190
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 212..227
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 249..262
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 263..283
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 284..310
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 332..352
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 353..373
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 374..376
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 398..420
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 421..441
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 442..490
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CARBOHYD 293
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 479
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 490 AA; 55337 MW; FA849E02DB42204D CRC64;
MEMANDKVAE TVIVGNYVEM ESEGKPPQDI KSKLSNFLWH GGSAYDAWFS CASNQVAQVL
LTLPYSFSQL GMLSGILFQL FYGILGSWTA YLISILYVEY RTRKEREKVN FRSHVIQWFE
VLDGLLGKHW RNVGLGFNCT FLLFGSVIQL IACASNIYYI NDNLDKRTWT YIFGACCATT
VFIPSFHNYR IWSFLGLVMT TYTAWYLTIA AVLHGQVEGV KHSGPNKIIL YFTGATNILY
TFGGHAVTVE IMHAMWKPQK FKAIYLLATL YVLTLTIPSA TAVYWAFGDM LLNHSNAFAL
LPKSPFRDMA VILMLIHQFI TFGFACTPLY FVWEKTVGMH ECKSLCKRAL VRLPVVIPIW
FLAIIFPFFG PINSTVGSLL VSFTVYIIPA LAHIFTFKSS SARQNAVEQP PKFVGRWVGT
FVINVFIVVW VLIVGFGFGG WASMVNFVHQ IDTFGLFTKC YQCPPPTPSV PTMPPHQMNA
TAPSPHHHHH
