LAYN_HUMAN
ID LAYN_HUMAN Reviewed; 382 AA.
AC Q6UX15; A6NJB0; B4DJU0; Q8TAY8; Q96NC5; Q96NF3;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Layilin;
DE Flags: Precursor;
GN Name=LAYN; ORFNames=UNQ208/PRO234;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
RC TISSUE=Thalamus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP FUNCTION.
RX PubMed=11294894; DOI=10.1091/mbc.12.4.891;
RA Bono P., Rubin K., Higgins J.M.G., Hynes R.O.;
RT "Layilin, a novel integral membrane protein, is a hyaluronan receptor.";
RL Mol. Biol. Cell 12:891-900(2001).
CC -!- FUNCTION: Receptor for hyaluronate. {ECO:0000269|PubMed:11294894}.
CC -!- SUBUNIT: Interacts with NF2, RDX and TLN1. {ECO:0000250}.
CC -!- INTERACTION:
CC Q6UX15-2; Q5J5C9: DEFB121; NbExp=3; IntAct=EBI-19944128, EBI-10244198;
CC Q6UX15-2; Q96F15: GIMAP5; NbExp=3; IntAct=EBI-19944128, EBI-6166686;
CC Q6UX15-2; P24593: IGFBP5; NbExp=3; IntAct=EBI-19944128, EBI-720480;
CC Q6UX15-2; O43934: MFSD11; NbExp=3; IntAct=EBI-19944128, EBI-17633886;
CC Q6UX15-2; Q8IY26: PLPP6; NbExp=3; IntAct=EBI-19944128, EBI-11721828;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}. Note=Colocalizes with TLN1 at the
CC membrane ruffles. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q6UX15-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6UX15-2; Sequence=VSP_021781;
CC Name=3;
CC IsoId=Q6UX15-3; Sequence=VSP_054660, VSP_054661;
CC -!- DOMAIN: The C-terminal domain interacts with the N-terminal domain of
CC RDX. {ECO:0000250}.
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DR EMBL; AY358554; AAQ88918.1; -; mRNA.
DR EMBL; AK055539; BAB70946.1; -; mRNA.
DR EMBL; AK055654; BAB70978.1; -; mRNA.
DR EMBL; AK296231; BAG58952.1; -; mRNA.
DR EMBL; AP002008; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471065; EAW67145.1; -; Genomic_DNA.
DR EMBL; BC025407; AAH25407.1; -; mRNA.
DR CCDS; CCDS31676.1; -. [Q6UX15-2]
DR CCDS; CCDS58178.1; -. [Q6UX15-1]
DR CCDS; CCDS58179.1; -. [Q6UX15-3]
DR RefSeq; NP_001245319.1; NM_001258390.1. [Q6UX15-1]
DR RefSeq; NP_001245320.1; NM_001258391.1. [Q6UX15-3]
DR RefSeq; NP_001305728.1; NM_001318799.1.
DR RefSeq; NP_849156.1; NM_178834.4. [Q6UX15-2]
DR RefSeq; XP_006718833.1; XM_006718770.3. [Q6UX15-3]
DR AlphaFoldDB; Q6UX15; -.
DR SMR; Q6UX15; -.
DR BioGRID; 126823; 7.
DR IntAct; Q6UX15; 7.
DR MINT; Q6UX15; -.
DR STRING; 9606.ENSP00000364765; -.
DR DrugBank; DB08818; Hyaluronic acid.
DR GlyGen; Q6UX15; 2 sites, 1 O-linked glycan (1 site).
DR iPTMnet; Q6UX15; -.
DR PhosphoSitePlus; Q6UX15; -.
DR SwissPalm; Q6UX15; -.
DR BioMuta; LAYN; -.
DR EPD; Q6UX15; -.
DR jPOST; Q6UX15; -.
DR MassIVE; Q6UX15; -.
DR MaxQB; Q6UX15; -.
DR PaxDb; Q6UX15; -.
DR PeptideAtlas; Q6UX15; -.
DR PRIDE; Q6UX15; -.
DR ProteomicsDB; 4403; -.
DR ProteomicsDB; 67550; -. [Q6UX15-1]
DR ProteomicsDB; 67551; -. [Q6UX15-2]
DR Antibodypedia; 32045; 375 antibodies from 27 providers.
DR DNASU; 143903; -.
DR Ensembl; ENST00000375614.7; ENSP00000364764.2; ENSG00000204381.12. [Q6UX15-2]
DR Ensembl; ENST00000375615.7; ENSP00000364765.3; ENSG00000204381.12. [Q6UX15-1]
DR Ensembl; ENST00000436913.6; ENSP00000392942.2; ENSG00000204381.12. [Q6UX15-3]
DR GeneID; 143903; -.
DR KEGG; hsa:143903; -.
DR MANE-Select; ENST00000375614.7; ENSP00000364764.2; NM_178834.5; NP_849156.1. [Q6UX15-2]
DR UCSC; uc001plp.3; human. [Q6UX15-1]
DR CTD; 143903; -.
DR DisGeNET; 143903; -.
DR GeneCards; LAYN; -.
DR HGNC; HGNC:29471; LAYN.
DR HPA; ENSG00000204381; Low tissue specificity.
DR MIM; 618843; gene.
DR neXtProt; NX_Q6UX15; -.
DR OpenTargets; ENSG00000204381; -.
DR PharmGKB; PA142671570; -.
DR VEuPathDB; HostDB:ENSG00000204381; -.
DR eggNOG; KOG4297; Eukaryota.
DR GeneTree; ENSGT00390000001844; -.
DR HOGENOM; CLU_045492_1_1_1; -.
DR InParanoid; Q6UX15; -.
DR OMA; VHSGEAT; -.
DR OrthoDB; 1005951at2759; -.
DR PhylomeDB; Q6UX15; -.
DR TreeFam; TF330715; -.
DR PathwayCommons; Q6UX15; -.
DR SignaLink; Q6UX15; -.
DR BioGRID-ORCS; 143903; 11 hits in 1068 CRISPR screens.
DR ChiTaRS; LAYN; human.
DR GenomeRNAi; 143903; -.
DR Pharos; Q6UX15; Tbio.
DR PRO; PR:Q6UX15; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q6UX15; protein.
DR Bgee; ENSG00000204381; Expressed in decidua and 156 other tissues.
DR ExpressionAtlas; Q6UX15; baseline and differential.
DR Genevisible; Q6UX15; HS.
DR GO; GO:0009986; C:cell surface; ISS:HGNC-UCL.
DR GO; GO:0005925; C:focal adhesion; HDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0001726; C:ruffle; ISS:HGNC-UCL.
DR GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR GO; GO:0005540; F:hyaluronic acid binding; IDA:HGNC-UCL.
DR Gene3D; 3.10.100.10; -; 1.
DR InterPro; IPR001304; C-type_lectin-like.
DR InterPro; IPR016186; C-type_lectin-like/link_sf.
DR InterPro; IPR016187; CTDL_fold.
DR Pfam; PF00059; Lectin_C; 1.
DR SMART; SM00034; CLECT; 1.
DR SUPFAM; SSF56436; SSF56436; 1.
DR PROSITE; PS50041; C_TYPE_LECTIN_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Disulfide bond; Glycoprotein; Lectin; Membrane;
KW Phosphoprotein; Reference proteome; Repeat; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..382
FT /note="Layilin"
FT /id="PRO_0000262508"
FT TOPO_DOM 22..235
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 257..382
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 45..185
FT /note="C-type lectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT REPEAT 340..344
FT /note="1-1"
FT REPEAT 350..354
FT /note="1-2"
FT REPEAT 356..359
FT /note="2-1"
FT REPEAT 371..375
FT /note="1-3"
FT REPEAT 377..380
FT /note="2-2"
FT REGION 266..285
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 330..374
FT /note="Interaction with NF2"
FT /evidence="ECO:0000250"
FT REGION 337..382
FT /note="Interaction with TLN1"
FT /evidence="ECO:0000250"
FT REGION 340..375
FT /note="3 X 5 AA repeats of E-S-G-X-V"
FT REGION 356..380
FT /note="2 X 4 AA repeats of N-X-I-Y"
FT MOD_RES 286
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8C351"
FT MOD_RES 299
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8C351"
FT CARBOHYD 117
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 71..184
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 150..176
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT VAR_SEQ 1..35
FT /note="MRPGTALQAVLLAVLLVGLRAATGRLLSASDLDLR -> MVTSGLGSGGVRR
FT NKAIAQPARTFMLGLMAAYHNL (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_054660"
FT VAR_SEQ 29..36
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_021781"
FT VAR_SEQ 36..188
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_054661"
FT VARIANT 66
FT /note="E -> K (in dbSNP:rs11827718)"
FT /id="VAR_029496"
FT CONFLICT 219
FT /note="A -> T (in Ref. 2; BAB70978)"
FT /evidence="ECO:0000305"
FT CONFLICT 344
FT /note="V -> M (in Ref. 5; AAH25407)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 382 AA; 43108 MW; 5843084E56E11325 CRC64;
MRPGTALQAV LLAVLLVGLR AATGRLLSAS DLDLRGGQPV CRGGTQRPCY KVIYFHDTSR
RLNFEEAKEA CRRDGGQLVS IESEDEQKLI EKFIENLLPS DGDFWIGLRR REEKQSNSTA
CQDLYAWTDG SISQFRNWYV DEPSCGSEVC VVMYHQPSAP AGIGGPYMFQ WNDDRCNMKN
NFICKYSDEK PAVPSREAEG EETELTTPVL PEETQEEDAK KTFKESREAA LNLAYILIPS
IPLLLLLVVT TVVCWVWICR KRKREQPDPS TKKQHTIWPS PHQGNSPDLE VYNVIRKQSE
ADLAETRPDL KNISFRVCSG EATPDDMSCD YDNMAVNPSE SGFVTLVSVE SGFVTNDIYE
FSPDQMGRSK ESGWVENEIY GY
