LAYN_MOUSE
ID LAYN_MOUSE Reviewed; 381 AA.
AC Q8C351; E9QQ23; Q3TMU7;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 4.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Layilin;
DE Flags: Precursor;
GN Name=Layn;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 1-212 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Lung, and Medulla oblongata;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP INTERACTION WITH NF2 AND RDX.
RX PubMed=15913605; DOI=10.1016/j.yexcr.2005.04.017;
RA Bono P., Cordero E., Johnson K., Borowsky M., Ramesh V., Jacks T.,
RA Hynes R.O.;
RT "Layilin, a cell surface hyaluronan receptor, interacts with merlin and
RT radixin.";
RL Exp. Cell Res. 308:177-187(2005).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-286 AND SER-299, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
CC -!- FUNCTION: Receptor for hyaluronate. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with TLN1 (By similarity). Interacts with NF2 and
CC RDX. {ECO:0000250, ECO:0000269|PubMed:15913605}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}. Note=Colocalizes with TLN1 at the
CC membrane ruffles. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8C351-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8C351-2; Sequence=VSP_021782, VSP_021783;
CC -!- DOMAIN: The C-terminal domain interacts with the N-terminal domain of
CC RDX.
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DR EMBL; AK086930; BAC39765.2; -; mRNA.
DR EMBL; AK165695; BAE38343.1; -; mRNA.
DR EMBL; AC119831; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS52791.1; -. [Q8C351-1]
DR RefSeq; NP_001028706.1; NM_001033534.1. [Q8C351-1]
DR PDB; 2K00; NMR; -; B=367-381.
DR PDBsum; 2K00; -.
DR AlphaFoldDB; Q8C351; -.
DR SMR; Q8C351; -.
DR ELM; Q8C351; -.
DR STRING; 10090.ENSMUSP00000096379; -.
DR GlyGen; Q8C351; 1 site.
DR iPTMnet; Q8C351; -.
DR PhosphoSitePlus; Q8C351; -.
DR jPOST; Q8C351; -.
DR MaxQB; Q8C351; -.
DR PaxDb; Q8C351; -.
DR PeptideAtlas; Q8C351; -.
DR PRIDE; Q8C351; -.
DR ProteomicsDB; 264843; -. [Q8C351-1]
DR ProteomicsDB; 264844; -. [Q8C351-2]
DR Antibodypedia; 32045; 375 antibodies from 27 providers.
DR Ensembl; ENSMUST00000098782; ENSMUSP00000096379; ENSMUSG00000060594. [Q8C351-1]
DR GeneID; 244864; -.
DR KEGG; mmu:244864; -.
DR UCSC; uc012gtc.1; mouse. [Q8C351-1]
DR CTD; 143903; -.
DR MGI; MGI:2685357; Layn.
DR VEuPathDB; HostDB:ENSMUSG00000060594; -.
DR eggNOG; KOG4297; Eukaryota.
DR GeneTree; ENSGT00390000001844; -.
DR HOGENOM; CLU_045492_1_1_1; -.
DR InParanoid; Q8C351; -.
DR OMA; VHSGEAT; -.
DR OrthoDB; 1005951at2759; -.
DR PhylomeDB; Q8C351; -.
DR TreeFam; TF330715; -.
DR BioGRID-ORCS; 244864; 0 hits in 72 CRISPR screens.
DR ChiTaRS; Layn; mouse.
DR EvolutionaryTrace; Q8C351; -.
DR PRO; PR:Q8C351; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q8C351; protein.
DR Bgee; ENSMUSG00000060594; Expressed in aortic valve and 113 other tissues.
DR ExpressionAtlas; Q8C351; baseline and differential.
DR Genevisible; Q8C351; MM.
DR GO; GO:0009986; C:cell surface; ISS:HGNC-UCL.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0001726; C:ruffle; ISS:HGNC-UCL.
DR GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR GO; GO:0005540; F:hyaluronic acid binding; ISS:HGNC-UCL.
DR Gene3D; 3.10.100.10; -; 1.
DR IDEAL; IID50232; -.
DR InterPro; IPR001304; C-type_lectin-like.
DR InterPro; IPR016186; C-type_lectin-like/link_sf.
DR InterPro; IPR016187; CTDL_fold.
DR Pfam; PF00059; Lectin_C; 1.
DR SMART; SM00034; CLECT; 1.
DR SUPFAM; SSF56436; SSF56436; 1.
DR PROSITE; PS50041; C_TYPE_LECTIN_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Disulfide bond; Glycoprotein; Lectin;
KW Membrane; Phosphoprotein; Reference proteome; Repeat; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..381
FT /note="Layilin"
FT /id="PRO_0000262509"
FT TOPO_DOM 25..235
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 257..381
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 45..185
FT /note="C-type lectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT REPEAT 340..344
FT /note="1-1"
FT REPEAT 350..354
FT /note="1-2"
FT REPEAT 356..359
FT /note="2-1"
FT REPEAT 371..375
FT /note="1-3"
FT REPEAT 377..380
FT /note="2-2"
FT REGION 330..374
FT /note="Interaction with NF2"
FT /evidence="ECO:0000269|PubMed:15913605"
FT REGION 337..381
FT /note="Interaction with TLN1"
FT /evidence="ECO:0000250"
FT REGION 340..375
FT /note="3 X 5 AA repeats of E-S-G-X-V"
FT REGION 356..380
FT /note="2 X 4 AA repeats of N-X-I-Y"
FT MOD_RES 286
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319"
FT MOD_RES 299
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319"
FT CARBOHYD 117
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 71..184
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 150..176
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT VAR_SEQ 136..137
FT /note="RN -> SG (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_021782"
FT VAR_SEQ 138..381
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_021783"
FT STRAND 372..376
FT /evidence="ECO:0007829|PDB:2K00"
SQ SEQUENCE 381 AA; 43269 MW; CF20C2EE708A5E07 CRC64;
MQPGAALQAM LLAVLLAKPR DSKGRLLSAS DLDPRGGQLV CRGGTRRPCY KVIYFHDAFQ
RLNFEEAKET CMEDGGQLVS IETEDEQRLI EKFIENLLAS DGDFWIGLKR LEEKQSNNTA
CQDLYAWTDG STSQFRNWYV DEPSCGSEVC VVMYHQPSAP PGIGGSYMFQ WNDDRCNMKN
NFICKYHDDK PSTTPSIWPG GEATEPATPL LPEETQKEDT KETFKERREA ALNLAYILIP
SIPLFLLLVV TSAVCWVWIC RRKREQTDPS TKEQHTIWPT PRQENSPNLD VYNVIRKQSE
ADLAEPRPDL KNISFRVCSG EAMPDDMSCD YENIAVNPSE SGFVTLASME SGFVTNDIYE
FSPDRMGRSK ESGWVENEIY Y
