LAZ5_ARATH
ID LAZ5_ARATH Reviewed; 1170 AA.
AC O48573;
DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 25-MAY-2022, entry version 150.
DE RecName: Full=Disease resistance protein LAZ5 {ECO:0000305};
DE EC=3.2.2.6 {ECO:0000255|PROSITE-ProRule:PRU00204};
DE AltName: Full=Protein LAZARUS 5 {ECO:0000303|PubMed:20949080};
DE AltName: Full=Protein TOLERANCE TO TOBACCO RINGSPOT VIRUS 1 {ECO:0000303|PubMed:22057987};
GN Name=LAZ5 {ECO:0000303|PubMed:20949080};
GN Synonyms=TTR1 {ECO:0000303|PubMed:22057987};
GN OrderedLocusNames=At5g44870 {ECO:0000312|Araport:AT5G44870};
GN ORFNames=K21C13.4 {ECO:0000312|EMBL:BAB10868.1},
GN T19K24.2 {ECO:0000312|EMBL:AAC79134.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION, INDUCTION, AND MUTAGENESIS OF ILE-287 AND GLY-811.
RX PubMed=20949080; DOI=10.1371/journal.ppat.1001137;
RA Palma K., Thorgrimsen S., Malinovsky F.G., Fiil B.K., Nielsen H.B.,
RA Brodersen P., Hofius D., Petersen M., Mundy J.;
RT "Autoimmunity in Arabidopsis acd11 is mediated by epigenetic regulation of
RT an immune receptor.";
RL PLoS Pathog. 6:E1001137-E1001137(2010).
RN [5]
RP FUNCTION.
RC STRAIN=cv. Est;
RX PubMed=22057987; DOI=10.1007/s10059-011-0101-z;
RA Nam M., Koh S., Kim S.U., Domier L.L., Jeon J.H., Kim H.G., Lee S.H.,
RA Bent A.F., Moon J.S.;
RT "Arabidopsis TTR1 causes LRR-dependent lethal systemic necrosis, rather
RT than systemic acquired resistance, to Tobacco ringspot virus.";
RL Mol. Cells 32:421-429(2011).
CC -!- FUNCTION: TIR-NB-LRR receptor-like protein that may play a role in
CC plant innate immunity. May trigger hypersensitive programmed cell death
CC in response to pathogen attack (PubMed:20949080). Involved in tolerance
CC to tobacco ringspot virus (TRSV) (PubMed:22057987).
CC {ECO:0000269|PubMed:20949080, ECO:0000269|PubMed:22057987}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + NAD(+) = ADP-D-ribose + H(+) + nicotinamide;
CC Xref=Rhea:RHEA:16301, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17154, ChEBI:CHEBI:57540, ChEBI:CHEBI:57967; EC=3.2.2.6;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16302;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00204};
CC -!- INDUCTION: Up-regulated by ASHH2 via epigenetic chromatin H3K36me3
CC regulation during the vegetative development.
CC {ECO:0000269|PubMed:20949080}.
CC -!- DOMAIN: The TIR domain mediates NAD(+) hydrolase (NADase) activity.
CC Self-association of TIR domains is required for NADase activity.
CC {ECO:0000255|PROSITE-ProRule:PRU00204}.
CC -!- MISCELLANEOUS: Plants over-expressing LAZ5 exhibit hypersensitive cell
CC death and do not survive (PubMed:20949080). In the ecotype Estland
CC (Est), which shows a sensitive response upon tobacco ringspot virus
CC (TRSV) inoculation, the TTR1 allele is involved in the development of
CC lethal systemic necrosis in response to TRSV (PubMed:22057987).
CC {ECO:0000269|PubMed:20949080, ECO:0000269|PubMed:22057987}.
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DR EMBL; AB010693; BAB10868.1; -; Genomic_DNA.
DR EMBL; AC002342; AAC79134.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95170.1; -; Genomic_DNA.
DR RefSeq; NP_199300.1; NM_123855.2.
DR AlphaFoldDB; O48573; -.
DR SMR; O48573; -.
DR STRING; 3702.AT5G44870.1; -.
DR PaxDb; O48573; -.
DR PRIDE; O48573; -.
DR ProteomicsDB; 250718; -.
DR EnsemblPlants; AT5G44870.1; AT5G44870.1; AT5G44870.
DR GeneID; 834517; -.
DR Gramene; AT5G44870.1; AT5G44870.1; AT5G44870.
DR KEGG; ath:AT5G44870; -.
DR Araport; AT5G44870; -.
DR TAIR; locus:2155322; AT5G44870.
DR eggNOG; ENOG502SI7S; Eukaryota.
DR HOGENOM; CLU_001561_0_3_1; -.
DR InParanoid; O48573; -.
DR OMA; PNIPNWE; -.
DR OrthoDB; 1113380at2759; -.
DR PhylomeDB; O48573; -.
DR PRO; PR:O48573; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; O48573; baseline and differential.
DR Genevisible; O48573; AT.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0050135; F:NAD(P)+ nucleosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0061809; F:NAD+ nucleotidase, cyclic ADP-ribose generating; IEA:UniProtKB-EC.
DR GO; GO:0051607; P:defense response to virus; IMP:TAIR.
DR GO; GO:0009626; P:plant-type hypersensitive response; IMP:TAIR.
DR GO; GO:0009615; P:response to virus; IMP:TAIR.
DR GO; GO:0007165; P:signal transduction; IEA:InterPro.
DR Gene3D; 1.10.8.430; -; 1.
DR Gene3D; 3.40.50.10140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR045344; C-JID.
DR InterPro; IPR044974; Disease_R_plants.
DR InterPro; IPR011713; Leu-rich_rpt_3.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000157; TIR_dom.
DR InterPro; IPR035897; Toll_tir_struct_dom_sf.
DR PANTHER; PTHR11017; PTHR11017; 1.
DR Pfam; PF20160; C-JID; 1.
DR Pfam; PF07725; LRR_3; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF01582; TIR; 1.
DR SMART; SM00255; TIR; 1.
DR SUPFAM; SSF52200; SSF52200; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50104; TIR; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Hydrolase; Leucine-rich repeat; NAD; Nucleotide-binding;
KW Plant defense; Reference proteome; Repeat.
FT CHAIN 1..1170
FT /note="Disease resistance protein LAZ5"
FT /id="PRO_0000433383"
FT DOMAIN 10..172
FT /note="TIR"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT DOMAIN 261..513
FT /note="NB-ARC"
FT /evidence="ECO:0000255"
FT REPEAT 595..616
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 622..645
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 646..670
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 677..700
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 723..747
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 761..785
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 790..813
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 815..837
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 838..861
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT REPEAT 862..885
FT /note="LRR 10"
FT /evidence="ECO:0000255"
FT REPEAT 888..904
FT /note="LRR 11"
FT /evidence="ECO:0000255"
FT REPEAT 905..930
FT /note="LRR 12"
FT /evidence="ECO:0000255"
FT REGION 193..219
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 84
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00204"
FT MUTAGEN 287
FT /note="I->N: In laz5-D2; dominant negative mutation
FT responsible for suppression of the acd11-dependent
FT autoimmune response."
FT /evidence="ECO:0000269|PubMed:20949080"
FT MUTAGEN 811
FT /note="G->E: In laz5-D3; dominant negative mutation
FT responsible for suppression of the acd11-dependent
FT autoimmune response."
FT /evidence="ECO:0000269|PubMed:20949080"
SQ SEQUENCE 1170 AA; 133398 MW; 16DD3292DB9ED589 CRC64;
MAASSEILPE SWQVFINFRG ADLRNGFISH LAGALTSAGI TYYIDTEEVP SEDLTVLFKR
IEESEIALSI FSSNYAESKW CLDELVKIME QVKKGKLRIM PVFFNVKPEE VREQNGEFGL
KLYGEGKSKR PNIPNWENAL RSVPSKIGLN LANFRNEKEL LDKIIDSIKK VLARITRASR
VAESLNGISK DSEAKNVDTF SPNSSDFPST SIDDDLSINS PQYQATIPPA SREGERLNTI
STVSSTGSIE HPPPNYGIEP RLKEMEEKLD FDSLETKTVG IVGMPGIGKT TLAETLYRKW
EHKFERSMFF PDASKMANEH GMCWLQKRLL EELLKDTNLN IGYTTNEHEF CKDVLLLKKV
FLVIDNVSSE EQIETLFGKW NWIKNGSKIV ITSSDESMLK GFVKDTYVVP SLNSRDSLLW
FTNHAFGLDD AQGNLVKLSK HFLNYAKGNP LALGAFGVEL CGKDKADWEK RIKTLTLISN
KMIQDVLRRR YDELTERQKD IFLDVACFFK SENESYVRHV VNSCDSESTK SWDEITDLKG
KFLVNISGGR VEMHDILCTF AKELASQALT EDTRVHLRLW NYQDIMWFLN NELEMENVRG
IFLDMSKVPE EMTFDGNIFS NMCNLRYLKI YSSVCHKEGE GIFKFDTVRE IQLPLDKVRY
LHWMKYPWEK LPSDFNPENL VDLELPYSSI KKVWEGVKDT PILKWANLSY SSKLTNLLGL
SNAKNLERLN LEGCTSLLKL PQEMENMKSL VFLNMRRCTS LTCLQSIKVS SLKILILSDC
SKLEEFEVIS ENLEELYLDG TAIKGLPPAA GDLTRLVVLN MEGCTELESL PKRLGKQKAL
QELVLSGCSK LESVPTDVKD MKHLRLLLLD GTRIRKIPKI KSLKCLCLSR NIAMVNLQDN
LKDFSNLKCL VMKNCENLRY LPSLPKCLEY LNVYGCERLE SVENPLVADR LTLFLDRSEE
LRSTFLFTNC HNLFQDAKDS ISTYAKWKCH RLAVECYEQD IVSGAFFNTC YPGYIVPSWF
DHQAVGSVLE PRLEPHWYNT MLSGIALCAV VSFHENQDPI IGSFSVKCTL QFENEDGSLR
FDCDIGCLNE PGMIEADHVF IGYVTCSRLK DHHSIPIHHP TTVKMQFHLT DACKSKVVDC
GFRLMYTQSR GCLLEEEVNA NFTKLYLGLL
