ID   LAZA_SCHAM              Reviewed;         214 AA.
AC   P49291;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Lazarillo protein;
DE   Flags: Precursor;
OS   Schistocerca americana (American grasshopper).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Polyneoptera; Orthoptera; Caelifera; Acrididea; Acridomorpha;
OC   Acridoidea; Acrididae; Cyrtacanthacridinae; Schistocerca.
OX   NCBI_TaxID=7009;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 46-51; 108-134 AND
RP   140-180.
RX   PubMed=7867494; DOI=10.1242/dev.121.1.123;
RA   Ganfornina M.D., Sanchez D., Bastiani M.J.;
RT   "Lazarillo, a new GPI-linked surface lipocalin, is restricted to a subset
RT   of neurons in the grasshopper embryo.";
RL   Development 121:123-134(1995).
RN   [2]
RP   POSSIBLE FUNCTION.
RX   PubMed=7867495; DOI=10.1242/dev.121.1.135;
RA   Sanchez D., Ganfornina M.D., Bastiani M.J.;
RT   "Developmental expression of the lipocalin Lazarillo and its role in axonal
RT   pathfinding in the grasshopper embryo.";
RL   Development 121:135-147(1995).
CC   -!- FUNCTION: Putative role in axonal outgrowth and guidance, required for
CC       the navigation of identified commissural neurons. Could be a receptor
CC       the midline morphogen.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
CC   -!- TISSUE SPECIFICITY: Expressed by a subset of neuroblasts, ganglion
CC       mother cells and neurons of the CNS; by all sensory neurons of the PNS.
CC   -!- PTM: N-glycosylated.
CC   -!- PTM: Contains disulfide bonds.
CC   -!- MISCELLANEOUS: Named after the main character of a 16th century Spanish
CC       novel, Lazarillo de Tormes, a crafty boy who guided a blind man.
CC   -!- SIMILARITY: Belongs to the calycin superfamily. Lipocalin family.
CC       {ECO:0000305}.
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DR   EMBL; Z38071; CAA86216.1; -; mRNA.
DR   EMBL; U15656; AAC46605.1; -; mRNA.
DR   AlphaFoldDB; P49291; -.
DR   SMR; P49291; -.
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0031409; F:pigment binding; IEA:InterPro.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.128.20; -; 1.
DR   InterPro; IPR012674; Calycin.
DR   InterPro; IPR003057; Invtbrt_color.
DR   InterPro; IPR022271; Lipocalin_ApoD.
DR   InterPro; IPR000566; Lipocln_cytosolic_FA-bd_dom.
DR   Pfam; PF00061; Lipocalin; 1.
DR   PIRSF; PIRSF036893; Lipocalin_ApoD; 1.
DR   PRINTS; PR01273; INVTBRTCOLOR.
DR   SUPFAM; SSF50814; SSF50814; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Developmental protein; Differentiation;
KW   Direct protein sequencing; Disulfide bond; Glycoprotein; GPI-anchor;
KW   Lipoprotein; Membrane; Neurogenesis; Receptor; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..192
FT                   /note="Lazarillo protein"
FT                   /id="PRO_0000017912"
FT   PROPEP          193..214
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000017913"
FT   LIPID           192
FT                   /note="GPI-anchor amidated alanine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        38
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        74
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        84
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        90
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        130
FT                   /note="N-linked (GlcNAc...) asparagine"
FT   CARBOHYD        158
FT                   /note="N-linked (GlcNAc...) asparagine"
FT   CARBOHYD        161
FT                   /note="N-linked (GlcNAc...) asparagine"
SQ   SEQUENCE   214 AA;  23641 MW;  6108AAE525BB3628 CRC64;
     MIRRGLLSVT AALVLLSVSC SAQETMGCAD RTAINDFNAT LYMGKWYEYA KMGSMPYEEG
     GVCVTAEYSM SSNNITVVNS MKDNTTHEVN TTTGWAEFAS ELHTDGKLSV HFPNSPSVGN
     YWILSTDYDN YSIVWSCVKR PDSAASTEIS WILLRSRNSS NMTLERVEDE LKNLQLDLNK
     YTKTEQSAKY CAGAEHVVGA MLSVAIASLF ALLH