LAZY1_ARATH
ID LAZY1_ARATH Reviewed; 358 AA.
AC Q5XV40; Q9FMU1;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Protein LAZY 1 {ECO:0000305};
DE Short=AtLA1 {ECO:0000303|PubMed:25028496};
DE Short=AtLAZY1 {ECO:0000303|PubMed:23331961};
DE AltName: Full=Protein LAZY1-LIKE 1 {ECO:0000303|PubMed:28765510};
GN Name=LAZY1 {ECO:0000303|PubMed:28821594};
GN Synonyms=LA1 {ECO:0000305}, LZY1 {ECO:0000303|PubMed:28765510};
GN OrderedLocusNames=At5g14090 {ECO:0000312|Araport:AT5G14090};
GN ORFNames=MUA22.9 {ECO:0000312|EMBL:BAB08288.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE,
RP NUCLEAR LOCALIZATION SIGNAL, AND MUTAGENESIS OF 252-LYS-LYS-253.
RX PubMed=23331961; DOI=10.1111/tpj.12118;
RA Yoshihara T., Spalding E.P., Iino M.;
RT "AtLAZY1 is a signaling component required for gravitropism of the
RT Arabidopsis thaliana inflorescence.";
RL Plant J. 74:267-279(2013).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=25028496; DOI=10.1073/pnas.1411859111;
RA Sang D., Chen D., Liu G., Liang Y., Huang L., Meng X., Chu J., Sun X.,
RA Dong G., Yuan Y., Qian Q., Li J., Wang Y.;
RT "Strigolactones regulate rice tiller angle by attenuating shoot
RT gravitropism through inhibiting auxin biosynthesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:11199-11204(2014).
RN [6]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=28765510; DOI=10.1105/tpc.16.00575;
RA Taniguchi M., Furutani M., Nishimura T., Nakamura M., Fushita T.,
RA Iijima K., Baba K., Tanaka H., Toyota M., Tasaka M., Morita M.T.;
RT "The Arabidopsis LAZY1 family plays a key role in gravity signaling within
RT statocytes and in branch angle control of roots and shoots.";
RL Plant Cell 29:1984-1999(2017).
RN [7]
RP FUNCTION.
RX PubMed=28821594; DOI=10.1104/pp.17.00942;
RA Yoshihara T., Spalding E.P.;
RT "LAZY genes mediate the effects of gravity on auxin gradients and plant
RT architecture.";
RL Plant Physiol. 175:959-969(2017).
RN [8]
RP MUTAGENESIS OF LEU-92 AND ILE-94.
RX PubMed=31818902; DOI=10.1104/pp.19.01144;
RA Yoshihara T., Spalding E.P.;
RT "Switching the direction of stem gravitropism by altering two amino acids
RT in AtLAZY1.";
RL Plant Physiol. 182:1039-1051(2020).
RN [9]
RP FUNCTION.
RX PubMed=32269265; DOI=10.1038/s41598-020-62962-4;
RA Hollender C.A., Hill J.L. Jr., Waite J., Dardick C.;
RT "Opposing influences of TAC1 and LAZY1 on lateral shoot orientation in
RT Arabidopsis.";
RL Sci. Rep. 10:6051-6051(2020).
CC -!- FUNCTION: Involved in the regulation of shoot gravitropism
CC (PubMed:23331961, PubMed:25028496). Involved in the regulation of
CC inflorescence branch angle (PubMed:23331961). Functions redundantly
CC with LAZY2, LAZY3 and LAZY4 to control plant architecture by coupling
CC gravity sensing to the formation of auxin gradients (PubMed:28821594).
CC Involved redundantly with LAZY2 and LAZY4 in the regulation of both
CC shoot and root gravitropism (PubMed:28765510). Mediates gravity
CC signaling in statocytes downstream of amyloplast displacement, leading
CC to the generation of asymmetric auxin distribution in gravity-
CC responding organs (PubMed:28765510). Regulates the direction of polar
CC auxin transport in response to gravity through the control of
CC asymmetric PIN3 expression in the root cap columella (PubMed:28765510).
CC Regulation of auxin flow by the three proteins LAZY1, LAZY2 and LAZY4
CC in statocytes influences plant architecture by controlling the growth
CC angle of lateral shoots and lateral roots (PubMed:28765510).
CC {ECO:0000269|PubMed:23331961, ECO:0000269|PubMed:25028496,
CC ECO:0000269|PubMed:28765510, ECO:0000269|PubMed:28821594}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:23331961};
CC Single-pass membrane protein {ECO:0000255}. Nucleus
CC {ECO:0000269|PubMed:23331961}.
CC -!- TISSUE SPECIFICITY: Expressed in shoot apical meristem, vascular
CC tissues, stele, endodermis and upper portion of the inflorescence stem
CC (PubMed:23331961). Expressed in the endodermis of inflorescence stems
CC and hypocotyls (PubMed:28765510). {ECO:0000269|PubMed:23331961,
CC ECO:0000269|PubMed:28765510}.
CC -!- DISRUPTION PHENOTYPE: Defective shoot gravitropism (PubMed:23331961,
CC PubMed:25028496). Increased angle of inflorescence branches
CC (PubMed:23331961). {ECO:0000269|PubMed:23331961,
CC ECO:0000269|PubMed:25028496}.
CC -!- SIMILARITY: Belongs to the LAZY family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08288.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB007650; BAB08288.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED91986.1; -; Genomic_DNA.
DR EMBL; AY735682; AAU44552.1; -; mRNA.
DR EMBL; AY773897; AAV63926.1; -; mRNA.
DR RefSeq; NP_196913.2; NM_121412.4.
DR AlphaFoldDB; Q5XV40; -.
DR STRING; 3702.AT5G14090.1; -.
DR iPTMnet; Q5XV40; -.
DR PaxDb; Q5XV40; -.
DR PRIDE; Q5XV40; -.
DR ProteomicsDB; 237073; -.
DR EnsemblPlants; AT5G14090.1; AT5G14090.1; AT5G14090.
DR GeneID; 831258; -.
DR Gramene; AT5G14090.1; AT5G14090.1; AT5G14090.
DR KEGG; ath:AT5G14090; -.
DR Araport; AT5G14090; -.
DR TAIR; locus:2174643; AT5G14090.
DR eggNOG; ENOG502R8CH; Eukaryota.
DR HOGENOM; CLU_040737_0_0_1; -.
DR InParanoid; Q5XV40; -.
DR OMA; RCIQYEL; -.
DR OrthoDB; 1304166at2759; -.
DR PhylomeDB; Q5XV40; -.
DR PRO; PR:Q5XV40; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q5XV40; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0009959; P:negative gravitropism; IGI:TAIR.
DR GO; GO:0009958; P:positive gravitropism; IMP:TAIR.
DR GO; GO:2000012; P:regulation of auxin polar transport; IEA:InterPro.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR InterPro; IPR038928; LAZY1.
DR PANTHER; PTHR34959; PTHR34959; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Growth regulation; Membrane; Nucleus; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..358
FT /note="Protein LAZY 1"
FT /id="PRO_0000437475"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOTIF 90..96
FT /note="IGT motif"
FT /evidence="ECO:0000305"
FT MOTIF 231..253
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000305|PubMed:23331961"
FT MUTAGEN 92
FT /note="L->A: Switches the shoot gravity responses from
FT upward bending to downward bending; when associated with A-
FT 94."
FT /evidence="ECO:0000269|PubMed:31818902"
FT MUTAGEN 94
FT /note="I->A: Switches the shoot gravity responses from
FT upward bending to downward bending; when associated with A-
FT 92."
FT /evidence="ECO:0000269|PubMed:31818902"
FT MUTAGEN 252..253
FT /note="KK->AA: Prevents targeting to the nucleus."
FT /evidence="ECO:0000269|PubMed:23331961"
SQ SEQUENCE 358 AA; 40512 MW; D78944A461BBFD6B CRC64;
MKFWGWMHHK FRENSKEPLK DASTGNSYSI LSAHPSLDSQ EVYPTACAGS RYNTGFRKQV
NLFQESSFAG PKQYTEEDFK DERNSDFFDG FLAIGTLGGE TLLDEQPATP TFGMSFEDPA
IDDADVTEND LKLISNELDK FLEAEAKEGH HQPSGRNSDT NTIASTIEAI EGVDDEEDNQ
PMKFPLQEYF FGSLIELPES KIAGKKDRAS LGELFQITEV QDKQSENIYG KKKKQPNSAH
KSAKHLVKKV LKKIHPSSRG SVSGKPEVDS TKKKFQKMVQ VFHRKVHPEE SIMETKIYSS
VANPKSSKAN SIDLTFEKVN HCHEASKRCI QYELRSSRSA KNGEHWIKTD EDYFVLEL
