LAZY4_ARATH
ID LAZY4_ARATH Reviewed; 287 AA.
AC Q5XVG3; Q9C9E7;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Protein LAZY 4 {ECO:0000303|PubMed:28821594};
DE Short=AtLAZY4 {ECO:0000303|PubMed:28821594};
DE AltName: Full=Protein DEEPER ROOTING 1 {ECO:0000303|PubMed:28029738};
DE Short=AtDRO1 {ECO:0000303|PubMed:28029738};
DE AltName: Full=Protein LAZY1-LIKE 3 {ECO:0000303|PubMed:28765510};
DE AltName: Full=Protein NEGATIVE GRAVITROPIC RESPONSE OF ROOTS 2 {ECO:0000303|PubMed:27748769};
DE Short=AtNGR2 {ECO:0000303|PubMed:27748769};
GN Name=LAZY4 {ECO:0000303|PubMed:28821594};
GN Synonyms=DRO1 {ECO:0000303|PubMed:28029738},
GN LZY3 {ECO:0000303|PubMed:28765510}, NGR2 {ECO:0000303|PubMed:27748769};
GN OrderedLocusNames=At1g72490 {ECO:0000312|Araport:AT1G72490};
GN ORFNames=T10D10.4 {ECO:0000312|EMBL:ANM60056.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=27748769; DOI=10.1038/nplants.2016.155;
RA Ge L., Chen R.;
RT "Negative gravitropism in plant roots.";
RL Nat. Plants 2:16155-16155(2016).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=28765510; DOI=10.1105/tpc.16.00575;
RA Taniguchi M., Furutani M., Nishimura T., Nakamura M., Fushita T.,
RA Iijima K., Baba K., Tanaka H., Toyota M., Tasaka M., Morita M.T.;
RT "The Arabidopsis LAZY1 family plays a key role in gravity signaling within
RT statocytes and in branch angle control of roots and shoots.";
RL Plant Cell 29:1984-1999(2017).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP 283-ILE--ILE-287.
RX PubMed=28029738; DOI=10.1111/tpj.13470;
RA Guseman J.M., Webb K., Srinivasan C., Dardick C.;
RT "DRO1 influences root system architecture in Arabidopsis and Prunus
RT species.";
RL Plant J. 89:1093-1105(2017).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=28821594; DOI=10.1104/pp.17.00942;
RA Yoshihara T., Spalding E.P.;
RT "LAZY genes mediate the effects of gravity on auxin gradients and plant
RT architecture.";
RL Plant Physiol. 175:959-969(2017).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=32130643; DOI=10.1007/s11103-020-00984-2;
RA Waite J.M., Collum T.D., Dardick C.;
RT "AtDRO1 is nuclear localized in root tips under native conditions and
RT impacts auxin localization.";
RL Plant Mol. Biol. 103:197-210(2020).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) OF 274-287.
RX PubMed=31900388; DOI=10.1038/s41467-019-13729-7;
RA Furutani M., Hirano Y., Nishimura T., Nakamura M., Taniguchi M., Suzuki K.,
RA Oshida R., Kondo C., Sun S., Kato K., Fukao Y., Hakoshima T., Morita M.T.;
RT "Polar recruitment of RLD by LAZY1-like protein during gravity signaling in
RT root branch angle control.";
RL Nat. Commun. 11:76-76(2020).
CC -!- FUNCTION: Involved in the regulation of root gravitropism
CC (PubMed:27748769). Functions redundantly with LAZY2 and LAZY3 in the
CC control of root gravitropism (PubMed:27748769). Involved in the
CC development of the root system architecture by influencing lateral root
CC angles and primary root length (PubMed:28029738, PubMed:32130643).
CC Functions redundantly with LAZY1, LAZY2 and LAZY3 to control plant
CC architecture by coupling gravity sensing to the formation of auxin
CC gradients (PubMed:28821594). Involved in the establishement of auxin
CC gradient in primary and lateral roots upon gravitropic stimulation
CC (PubMed:32130643). Involved redundantly with LAZY1 and LAZY2 in the
CC regulation of both shoot and root gravitropism (PubMed:28765510).
CC Mediates gravity signaling in statocytes downstream of amyloplast
CC displacement, leading to the generation of asymmetric auxin
CC distribution in gravity-responding organs (PubMed:28765510). Regulates
CC the direction of polar auxin transport in response to gravity through
CC the control of asymmetric PIN3 expression in the root cap columella
CC (PubMed:28765510). Regulation of auxin flow by the three proteins
CC LAZY1, LAZY2 and LAZY4 in statocytes influences plant architecture by
CC controlling the growth angle of lateral shoots and lateral roots
CC (PubMed:28765510). {ECO:0000269|PubMed:27748769,
CC ECO:0000269|PubMed:28029738, ECO:0000269|PubMed:28765510,
CC ECO:0000269|PubMed:28821594, ECO:0000269|PubMed:32130643}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:32130643}.
CC Note=Localizes in the nuclei of cortical and endodermal tissues near
CC root tips in both primary and lateral roots.
CC {ECO:0000269|PubMed:32130643}.
CC -!- TISSUE SPECIFICITY: Specifically expressed in roots (PubMed:28029738).
CC Expressed in root tips (PubMed:32130643). Expressed in the endodermis
CC of inflorescence stems and hypocotyls (PubMed:28765510). Highly
CC expressed in the lower hypocotyl and root stele of young seedlings
CC (PubMed:28821594). {ECO:0000269|PubMed:28029738,
CC ECO:0000269|PubMed:28765510, ECO:0000269|PubMed:28821594,
CC ECO:0000269|PubMed:32130643}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions (PubMed:27748769). Roots of plants lacking LAZY2, LAZY3 and
CC LAZY4 exhibit a negative gravitropic response, and grow upward in the
CC opposite direrction of root gravitropism (PubMed:27748769). Increased
CC branch angles of lateral roots and reduced length of primary root
CC (PubMed:28029738, PubMed:32130643). {ECO:0000269|PubMed:27748769,
CC ECO:0000269|PubMed:28029738, ECO:0000269|PubMed:32130643}.
CC -!- MISCELLANEOUS: Plants overexpressing LAZY4 exhibit steep lateral root
CC angles, upward leaf curling, shortened siliques and narrow lateral
CC branch angles. {ECO:0000269|PubMed:28029738}.
CC -!- SIMILARITY: Belongs to the LAZY family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG52588.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; KT021797; ANJ86424.1; -; mRNA.
DR EMBL; AC016529; AAG52588.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35330.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60056.1; -; Genomic_DNA.
DR EMBL; AY735558; AAU44428.1; -; mRNA.
DR EMBL; AY735559; AAU44429.1; -; mRNA.
DR EMBL; AY924725; AAX23800.1; -; mRNA.
DR PIR; B96749; B96749.
DR RefSeq; NP_001322368.1; NM_001334534.1.
DR RefSeq; NP_177393.2; NM_105908.4.
DR PDB; 6L0V; X-ray; 1.35 A; B/D/F/H=274-287.
DR PDB; 6L0W; X-ray; 1.59 A; B/D=274-287.
DR PDBsum; 6L0V; -.
DR PDBsum; 6L0W; -.
DR AlphaFoldDB; Q5XVG3; -.
DR SMR; Q5XVG3; -.
DR STRING; 3702.AT1G72490.1; -.
DR PaxDb; Q5XVG3; -.
DR PRIDE; Q5XVG3; -.
DR EnsemblPlants; AT1G72490.1; AT1G72490.1; AT1G72490.
DR EnsemblPlants; AT1G72490.2; AT1G72490.2; AT1G72490.
DR GeneID; 843581; -.
DR Gramene; AT1G72490.1; AT1G72490.1; AT1G72490.
DR Gramene; AT1G72490.2; AT1G72490.2; AT1G72490.
DR KEGG; ath:AT1G72490; -.
DR Araport; AT1G72490; -.
DR TAIR; locus:2194012; AT1G72490.
DR eggNOG; ENOG502RRH5; Eukaryota.
DR HOGENOM; CLU_068790_0_0_1; -.
DR InParanoid; Q5XVG3; -.
DR OMA; AIYTESK; -.
DR PhylomeDB; Q5XVG3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q5XVG3; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0009630; P:gravitropism; IMP:TAIR.
DR GO; GO:0080181; P:lateral root branching; IMP:TAIR.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR InterPro; IPR044683; LAZY.
DR PANTHER; PTHR34045; PTHR34045; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Growth regulation; Nucleus; Reference proteome.
FT CHAIN 1..287
FT /note="Protein LAZY 4"
FT /id="PRO_0000451020"
FT REGION 15..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 71..99
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 236..280
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 41..47
FT /note="IGT motif"
FT COMPBIAS 236..254
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 255..274
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 283..287
FT /note="Missing: No shoot and root phenotypes when
FT ectopically expressed in plants."
FT /evidence="ECO:0000269|PubMed:28029738"
FT STRAND 274..276
FT /evidence="ECO:0007829|PDB:6L0V"
FT STRAND 280..286
FT /evidence="ECO:0007829|PDB:6L0V"
SQ SEQUENCE 287 AA; 33055 MW; 3513DD0F1E5A5BBB CRC64;
MKFFGWMQNK LHGKQEITHR PSISSASSHH PREEFNDWPH GLLAIGTFGN KKQTPQTLDQ
EVIQEETVSN LHVEGRQAQD TDQELSSSDD LEEDFTPEEV GKLQKELTKL LTRRSKKRKS
DVNRELANLP LDRFLNCPSS LEVDRRISNA LCDEKEEDIE RTISVILGRC KAISTESKNK
TKKNKRDLSK TSVSHLLKKM FVCTEGFSPV PRPILRDTFQ ETRMEKLLRM MLHKKVNTQA
SSKQTSTKKY LQDKQQLSLK NEEEEGRSSN DGGKWVKTDS DFIVLEI
