LA_DROME
ID LA_DROME Reviewed; 390 AA.
AC P40796; Q24375; Q9VIN2;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=La protein homolog;
DE AltName: Full=La autoantigen homolog;
DE AltName: Full=La ribonucleoprotein;
GN Name=La; ORFNames=CG10922;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Canton-S; TISSUE=Ovary;
RX PubMed=8035794; DOI=10.1128/mcb.14.8.5123-5129.1994;
RA Bai C., Li Z., Tolias P.P.;
RT "Developmental characterization of a Drosophila RNA-binding protein
RT homologous to the human systemic lupus erythematosus-associated La/SS-B
RT autoantigen.";
RL Mol. Cell. Biol. 14:5123-5129(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8035818; DOI=10.1128/mcb.14.8.5412-5424.1994;
RA Yoo C.J., Wolin S.L.;
RT "La proteins from Drosophila melanogaster and Saccharomyces cerevisiae: a
RT yeast homolog of the La autoantigen is dispensable for growth.";
RL Mol. Cell. Biol. 14:5412-5424(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-7 AND SER-9, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=17372656; DOI=10.1039/b617545g;
RA Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A.,
RA Eng J.K., Aebersold R., Tao W.A.;
RT "An integrated chemical, mass spectrometric and computational strategy for
RT (quantitative) phosphoproteomics: application to Drosophila melanogaster
RT Kc167 cells.";
RL Mol. Biosyst. 3:275-286(2007).
CC -!- FUNCTION: May be involved in transcription termination by RNA
CC polymerase III. Binds RNA and DNA. Binds to precursors of RNA
CC polymerase III transcripts. May play a specialized role during fly
CC development.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout embryonic, larval, pupal, and
CC adult development. Expression throughout the embryo is followed by a
CC restricted pattern of mesodermal expression that is later confined to
CC the visceral mesoderm, gonads, gut, and salivary glands.
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DR EMBL; U07652; AAA20518.1; -; mRNA.
DR EMBL; L32988; AAA21776.1; -; mRNA.
DR EMBL; AE014134; AAF53885.1; -; Genomic_DNA.
DR PIR; A53773; A53773.
DR PIR; A53781; A53781.
DR RefSeq; NP_477014.1; NM_057666.5.
DR AlphaFoldDB; P40796; -.
DR SMR; P40796; -.
DR BioGRID; 61271; 9.
DR DIP; DIP-19001N; -.
DR IntAct; P40796; 10.
DR STRING; 7227.FBpp0080905; -.
DR iPTMnet; P40796; -.
DR PaxDb; P40796; -.
DR PRIDE; P40796; -.
DR DNASU; 35305; -.
DR EnsemblMetazoa; FBtr0081374; FBpp0080906; FBgn0011638.
DR GeneID; 35305; -.
DR KEGG; dme:Dmel_CG10922; -.
DR CTD; 35305; -.
DR FlyBase; FBgn0011638; La.
DR VEuPathDB; VectorBase:FBgn0011638; -.
DR eggNOG; KOG4213; Eukaryota.
DR GeneTree; ENSGT00830000128380; -.
DR HOGENOM; CLU_042341_1_1_1; -.
DR InParanoid; P40796; -.
DR OMA; PEHNEER; -.
DR OrthoDB; 882725at2759; -.
DR PhylomeDB; P40796; -.
DR SignaLink; P40796; -.
DR BioGRID-ORCS; 35305; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 35305; -.
DR PRO; PR:P40796; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0011638; Expressed in adult abdomen and 74 other tissues.
DR ExpressionAtlas; P40796; baseline and differential.
DR Genevisible; P40796; DM.
DR GO; GO:0000791; C:euchromatin; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:1990904; C:ribonucleoprotein complex; IEA:InterPro.
DR GO; GO:0008098; F:5S rRNA primary transcript binding; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003729; F:mRNA binding; ISS:FlyBase.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0008033; P:tRNA processing; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR045180; La_dom_prot.
DR InterPro; IPR006630; La_HTH.
DR InterPro; IPR014886; La_xRRM.
DR InterPro; IPR002344; Lupus_La.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR22792; PTHR22792; 1.
DR Pfam; PF05383; La; 1.
DR Pfam; PF08777; RRM_3; 1.
DR PRINTS; PR00302; LUPUSLA.
DR SMART; SM00715; LA; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF54928; SSF54928; 2.
DR PROSITE; PS50961; HTH_LA; 1.
DR PROSITE; PS50102; RRM; 1.
DR PROSITE; PS51939; XRRM; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Phosphoprotein; Reference proteome; RNA-binding.
FT CHAIN 1..390
FT /note="La protein homolog"
FT /id="PRO_0000207606"
FT DOMAIN 44..137
FT /note="HTH La-type RNA-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00332"
FT DOMAIN 149..234
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 259..386
FT /note="xRRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01288"
FT REGION 26..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 362..390
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 362..381
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 7
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT MOD_RES 9
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT CONFLICT 169
FT /note="A -> T (in Ref. 1; AAA20518)"
FT /evidence="ECO:0000305"
FT CONFLICT 182..183
FT /note="KH -> NS (in Ref. 1; AAA20518)"
FT /evidence="ECO:0000305"
FT CONFLICT 283
FT /note="A -> R (in Ref. 1; AAA20518)"
FT /evidence="ECO:0000305"
FT CONFLICT 329
FT /note="K -> N (in Ref. 1; AAA20518)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 390 AA; 44885 MW; A8099288B90446A5 CRC64;
MAEVAETPSV EAQEEVAQPA EAQVLEAKNG DAKKDPAPAA EEAAGGFTKQ ERAIIRQVEY
YFGDANLNRD KFLREQIGKN EDGWVPLSVL VTFKRLASLS TDLSEIVAAL NKSEEGLVEI
SEDKLSLRRH PERPIPEHNE ERRKEIQERT AYAKGFPLDS QISELLDFAA NYDKVVNLTM
RKHYDKPTKS YKFKGSIFLT FETKDQAKAF LEQEKIVYKE RELLRKWQVD YLKEKQEEYA
QKNEKRKNKK EAKPEPAFEL PKNAIVVFEG APETSSREEI REAFEKIKDF EVAYIEFAKG
ETKGSVRLTE ADAAEKYIAK VEEGKLKFKD EVSLSLRKAT EEEEKEFIDK AIEFMKKRRD
FTRNKGKRFN RKRHGGNDHK HGGGKKARGD
