LBD16_ARATH
ID LBD16_ARATH Reviewed; 245 AA.
AC Q9SLB7; B7XG72; Q0WLD7;
DT 28-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=LOB domain-containing protein 16;
DE AltName: Full=ASYMMETRIC LEAVES 2-like protein 18;
DE Short=AS2-like protein 18;
GN Name=LBD16; Synonyms=ASL18; OrderedLocusNames=At2g42430; ORFNames=MHK10.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=12068116; DOI=10.1104/pp.010926;
RA Shuai B., Reynaga-Pena C.G., Springer P.S.;
RT "The LATERAL ORGAN BOUNDARIES gene defines a novel, plant-specific gene
RT family.";
RL Plant Physiol. 129:747-761(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=19154202; DOI=10.1111/j.1365-313x.2009.03797.x;
RA Matsumura Y., Iwakawa H., Machida Y., Machida C.;
RT "Characterization of genes in the ASYMMETRIC LEAVES2/LATERAL ORGAN
RT BOUNDARIES (AS2/LOB) family in Arabidopsis thaliana, and functional and
RT molecular comparisons between AS2 and other family members.";
RL Plant J. 58:525-537(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=12481096; DOI=10.1104/pp.010207;
RA Xiao Y.-L., Malik M., Whitelaw C.A., Town C.D.;
RT "Cloning and sequencing of cDNAs for hypothetical genes from chromosome 2
RT of Arabidopsis.";
RL Plant Physiol. 130:2118-2128(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12040093; DOI=10.1093/pcp/pcf077;
RA Iwakawa H., Ueno Y., Semiarti E., Onouchi H., Kojima S., Tsukaya H.,
RA Hasebe M., Soma T., Ikezaki M., Machida C., Machida Y.;
RT "The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana, required for
RT formation of a symmetric flat leaf lamina, encodes a member of a novel
RT family of proteins characterized by cysteine repeats and a leucine
RT zipper.";
RL Plant Cell Physiol. 43:467-478(2002).
RN [9]
RP INDUCTION BY AUXIN.
RX PubMed=15659631; DOI=10.1105/tpc.104.028316;
RA Okushima Y., Overvoorde P.J., Arima K., Alonso J.M., Chan A., Chang C.,
RA Ecker J.R., Hughes B., Lui A., Nguyen D., Onodera C., Quach H., Smith A.,
RA Yu G., Theologis A.;
RT "Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family
RT members in Arabidopsis thaliana: unique and overlapping functions of ARF7
RT and ARF19.";
RL Plant Cell 17:444-463(2005).
RN [10]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION BY AUXIN.
RX PubMed=17259263; DOI=10.1105/tpc.106.047761;
RA Okushima Y., Fukaki H., Onoda M., Theologis A., Tasaka M.;
RT "ARF7 and ARF19 regulate lateral root formation via direct activation of
RT LBD/ASL genes in Arabidopsis.";
RL Plant Cell 19:118-130(2007).
RN [11]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=19717544; DOI=10.1104/pp.109.143685;
RA Lee H.W., Kim N.Y., Lee D.J., Kim J.;
RT "LBD18/ASL20 regulates lateral root formation in combination with
RT LBD16/ASL18 downstream of ARF7 and ARF19 in Arabidopsis.";
RL Plant Physiol. 151:1377-1389(2009).
RN [12]
RP SUBUNIT, AND MUTAGENESIS OF PRO-87.
RX PubMed=23430048; DOI=10.1093/mp/sst037;
RA Lee H.W., Kim M.J., Park M.Y., Han K.H., Kim J.;
RT "The conserved proline residue in the LOB domain of LBD18 is critical for
RT DNA-binding and biological function.";
RL Mol. Plant 6:1722-1725(2013).
RN [13]
RP FUNCTION, AND INDUCTION BY AUXIN.
RX PubMed=23749813; DOI=10.1093/pcp/pct081;
RA Kang N.Y., Lee H.W., Kim J.;
RT "The AP2/EREBP gene PUCHI Co-Acts with LBD16/ASL18 and LBD18/ASL20
RT downstream of ARF7 and ARF19 to regulate lateral root development in
RT Arabidopsis.";
RL Plant Cell Physiol. 54:1326-1334(2013).
RN [14]
RP FUNCTION.
RX PubMed=22974309; DOI=10.1111/tpj.12013;
RA Lee H.W., Kim M.J., Kim N.Y., Lee S.H., Kim J.;
RT "LBD18 acts as a transcriptional activator that directly binds to the
RT EXPANSIN14 promoter in promoting lateral root emergence of Arabidopsis.";
RL Plant J. 73:212-224(2013).
RN [15]
RP FUNCTION.
RX PubMed=26059335; DOI=10.1104/pp.15.00578;
RA Lee H.W., Cho C., Kim J.;
RT "Lateral organ boundaries domain16 and 18 act downstream of the AUXIN1 and
RT LIKE-AUXIN3 auxin influx carriers to control lateral root development in
RT Arabidopsis.";
RL Plant Physiol. 168:1792-1806(2015).
CC -!- FUNCTION: Transcriptional activator (PubMed:19717544, PubMed:22974309).
CC Involved in lateral root formation. Regulated by the transcriptional
CC activators ARF7 and ARF19 (PubMed:17259263). Functions in the
CC initiation and emergence of lateral roots, in conjunction with LBD18,
CC downstream of ARF7 and ARF19 (PubMed:19717544, PubMed:23749813). Acts
CC downstream of the auxin influx carriers AUX1 and LAX1 in the regulation
CC of lateral root initiation and development (PubMed:26059335).
CC {ECO:0000269|PubMed:17259263, ECO:0000269|PubMed:19717544,
CC ECO:0000269|PubMed:22974309, ECO:0000269|PubMed:23749813,
CC ECO:0000269|PubMed:26059335}.
CC -!- SUBUNIT: Homodimer and heterodimer with LBD18.
CC {ECO:0000269|PubMed:23430048}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17259263}.
CC -!- TISSUE SPECIFICITY: Expressed in roots and faintly in shoots.
CC {ECO:0000269|PubMed:12068116}.
CC -!- DEVELOPMENTAL STAGE: During lateral root formation, expressed in the
CC lateral root primordia, and the developing, emerged, and mature lateral
CC roots. {ECO:0000269|PubMed:19717544}.
CC -!- INDUCTION: By auxin. {ECO:0000269|PubMed:15659631,
CC ECO:0000269|PubMed:17259263, ECO:0000269|PubMed:23749813}.
CC -!- DISRUPTION PHENOTYPE: Reduced number of lateral roots.
CC {ECO:0000269|PubMed:19717544}.
CC -!- SIMILARITY: Belongs to the LOB domain-containing protein family.
CC {ECO:0000305}.
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DR EMBL; AF447890; AAL38035.1; -; mRNA.
DR EMBL; AB473851; BAH10562.1; -; mRNA.
DR EMBL; AC005956; AAD23725.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10119.1; -; Genomic_DNA.
DR EMBL; AF345339; AAK31143.1; -; mRNA.
DR EMBL; AF410334; AAK95320.1; -; mRNA.
DR EMBL; BT000866; AAN38703.1; -; mRNA.
DR EMBL; AK230268; BAF02070.1; -; mRNA.
DR PIR; G84853; G84853.
DR RefSeq; NP_565973.1; NM_129804.4.
DR AlphaFoldDB; Q9SLB7; -.
DR SMR; Q9SLB7; -.
DR BioGRID; 4180; 5.
DR IntAct; Q9SLB7; 3.
DR STRING; 3702.AT2G42430.1; -.
DR PaxDb; Q9SLB7; -.
DR PRIDE; Q9SLB7; -.
DR ProteomicsDB; 237074; -.
DR EnsemblPlants; AT2G42430.1; AT2G42430.1; AT2G42430.
DR GeneID; 818843; -.
DR Gramene; AT2G42430.1; AT2G42430.1; AT2G42430.
DR KEGG; ath:AT2G42430; -.
DR Araport; AT2G42430; -.
DR TAIR; locus:2053807; AT2G42430.
DR eggNOG; ENOG502QRSE; Eukaryota.
DR HOGENOM; CLU_058353_3_4_1; -.
DR InParanoid; Q9SLB7; -.
DR OMA; HREMESN; -.
DR OrthoDB; 1319833at2759; -.
DR PhylomeDB; Q9SLB7; -.
DR PRO; PR:Q9SLB7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SLB7; baseline and differential.
DR Genevisible; Q9SLB7; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:1990841; F:promoter-specific chromatin binding; IPI:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:1990110; P:callus formation; IMP:TAIR.
DR GO; GO:0009755; P:hormone-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0010311; P:lateral root formation; IMP:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR InterPro; IPR004883; LOB.
DR Pfam; PF03195; LOB; 1.
DR PROSITE; PS50891; LOB; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..245
FT /note="LOB domain-containing protein 16"
FT /id="PRO_0000132267"
FT DOMAIN 14..116
FT /note="LOB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00291"
FT REGION 162..183
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 163..183
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 87
FT /note="P->L: Loss of function in lateral root formation."
FT /evidence="ECO:0000269|PubMed:23430048"
SQ SEQUENCE 245 AA; 26465 MW; 8EE245111B8D7E55 CRC64;
MASSGNGTTA GTGSPCGACK FLRRKCASDC IFAPYFSSEQ GAARFAAIHK VFGASNVSKL
LLNVPIHDRC EAVVTIAYEA QARLHDPVYG CVSHIFALQQ QVAFLQSQVM QMKAQIAGHQ
TSAAGDLRHS SESTNQFMTW QQTSVSPIGS AYSTPYNHHQ PYYGHVNPNN PVSPQSSLEE
SFSNTSSDVT TTANVRETHH QTGGGVYGHD GIGFHEGYPN KKRSVSYCSS DLGELQALAL
RMMKN
