LBD18_ARATH
ID LBD18_ARATH Reviewed; 262 AA.
AC O22131; B7XG74; Q1ECL8; Q8W1D7;
DT 17-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=LOB domain-containing protein 18;
DE AltName: Full=ASYMMETRIC LEAVES 2-like protein 20;
DE Short=AS2-like protein 20;
GN Name=LBD18; Synonyms=ASL20; OrderedLocusNames=At2g45420; ORFNames=F4L23.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=12068116; DOI=10.1104/pp.010926;
RA Shuai B., Reynaga-Pena C.G., Springer P.S.;
RT "The LATERAL ORGAN BOUNDARIES gene defines a novel, plant-specific gene
RT family.";
RL Plant Physiol. 129:747-761(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=19154202; DOI=10.1111/j.1365-313x.2009.03797.x;
RA Matsumura Y., Iwakawa H., Machida Y., Machida C.;
RT "Characterization of genes in the ASYMMETRIC LEAVES2/LATERAL ORGAN
RT BOUNDARIES (AS2/LOB) family in Arabidopsis thaliana, and functional and
RT molecular comparisons between AS2 and other family members.";
RL Plant J. 58:525-537(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clone.";
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12040093; DOI=10.1093/pcp/pcf077;
RA Iwakawa H., Ueno Y., Semiarti E., Onouchi H., Kojima S., Tsukaya H.,
RA Hasebe M., Soma T., Ikezaki M., Machida C., Machida Y.;
RT "The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana, required for
RT formation of a symmetric flat leaf lamina, encodes a member of a novel
RT family of proteins characterized by cysteine repeats and a leucine
RT zipper.";
RL Plant Cell Physiol. 43:467-478(2002).
RN [7]
RP INDUCTION BY AUXIN.
RX PubMed=15659631; DOI=10.1105/tpc.104.028316;
RA Okushima Y., Overvoorde P.J., Arima K., Alonso J.M., Chan A., Chang C.,
RA Ecker J.R., Hughes B., Lui A., Nguyen D., Onodera C., Quach H., Smith A.,
RA Yu G., Theologis A.;
RT "Functional genomic analysis of the AUXIN RESPONSE FACTOR gene family
RT members in Arabidopsis thaliana: unique and overlapping functions of ARF7
RT and ARF19.";
RL Plant Cell 17:444-463(2005).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=19088331; DOI=10.1105/tpc.108.061796;
RA Soyano T., Thitamadee S., Machida Y., Chua N.-H.;
RT "ASYMMETRIC LEAVES2-LIKE19/LATERAL ORGAN BOUNDARIES DOMAIN30 and
RT ASL20/LBD18 regulate tracheary element differentiation in Arabidopsis.";
RL Plant Cell 20:3359-3373(2008).
RN [9]
RP FUNCTION, DEVELOPMENTAL STAGE, INDUCTION BY AUXIN, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=19717544; DOI=10.1104/pp.109.143685;
RA Lee H.W., Kim N.Y., Lee D.J., Kim J.;
RT "LBD18/ASL20 regulates lateral root formation in combination with
RT LBD16/ASL18 downstream of ARF7 and ARF19 in Arabidopsis.";
RL Plant Physiol. 151:1377-1389(2009).
RN [10]
RP SUBUNIT, AND MUTAGENESIS OF PRO-109.
RX PubMed=23430048; DOI=10.1093/mp/sst037;
RA Lee H.W., Kim M.J., Park M.Y., Han K.H., Kim J.;
RT "The conserved proline residue in the LOB domain of LBD18 is critical for
RT DNA-binding and biological function.";
RL Mol. Plant 6:1722-1725(2013).
RN [11]
RP FUNCTION, AND INDUCTION BY AUXIN.
RX PubMed=23749813; DOI=10.1093/pcp/pct081;
RA Kang N.Y., Lee H.W., Kim J.;
RT "The AP2/EREBP gene PUCHI Co-Acts with LBD16/ASL18 and LBD18/ASL20
RT downstream of ARF7 and ARF19 to regulate lateral root development in
RT Arabidopsis.";
RL Plant Cell Physiol. 54:1326-1334(2013).
RN [12]
RP FUNCTION.
RX PubMed=23872272; DOI=10.1093/pcp/pct105;
RA Lee H.W., Kim J.;
RT "EXPANSINA17 up-regulated by LBD18/ASL20 promotes lateral root formation
RT during the auxin response.";
RL Plant Cell Physiol. 54:1600-1611(2013).
RN [13]
RP FUNCTION.
RX PubMed=22974309; DOI=10.1111/tpj.12013;
RA Lee H.W., Kim M.J., Kim N.Y., Lee S.H., Kim J.;
RT "LBD18 acts as a transcriptional activator that directly binds to the
RT EXPANSIN14 promoter in promoting lateral root emergence of Arabidopsis.";
RL Plant J. 73:212-224(2013).
RN [14]
RP INTERACTION WITH GIP1, AND MUTAGENESIS OF PRO-109.
RX PubMed=24484953; DOI=10.1016/j.jplph.2013.11.003;
RA Lee H.W., Park J.H., Park M.Y., Kim J.;
RT "GIP1 may act as a coactivator that enhances transcriptional activity of
RT LBD18 in Arabidopsis.";
RL J. Plant Physiol. 171:14-18(2014).
RN [15]
RP FUNCTION.
RX PubMed=26059335; DOI=10.1104/pp.15.00578;
RA Lee H.W., Cho C., Kim J.;
RT "Lateral organ boundaries domain16 and 18 act downstream of the AUXIN1 and
RT LIKE-AUXIN3 auxin influx carriers to control lateral root development in
RT Arabidopsis.";
RL Plant Physiol. 168:1792-1806(2015).
CC -!- FUNCTION: Involved in the positive regulation of tracheary element (TE)
CC differentiation. Involved in a positive feedback loop that maintains or
CC promotes NAC030/VND7 expression that regulates TE differentiation-
CC related genes (PubMed:19088331). Functions in the initiation and
CC emergence of lateral roots, in conjunction with LBD16, downstream of
CC ARF7 and ARF19 (PubMed:19717544, PubMed:23749813). Transcriptional
CC activator that directly regulates EXPA14, a gene encoding a cell wall-
CC loosening factor that promotes lateral root emergence. Activates EXPA14
CC by directly binding to a specific region of its promoter
CC (PubMed:22974309). Transcriptional activator that directly regulates
CC EXPA17, a gene encoding a cell wall-loosening factor that promotes
CC lateral root emergence (PubMed:23872272). Acts downstream of the auxin
CC influx carriers AUX1 and LAX1 in the regulation of lateral root
CC initiation and development (PubMed:26059335).
CC {ECO:0000269|PubMed:19088331, ECO:0000269|PubMed:19717544,
CC ECO:0000269|PubMed:22974309, ECO:0000269|PubMed:23749813,
CC ECO:0000269|PubMed:23872272, ECO:0000269|PubMed:26059335}.
CC -!- SUBUNIT: Homodimer and heterodimer with LBD16 (PubMed:23430048).
CC Interacts with GIP1 (PubMed:24484953). {ECO:0000269|PubMed:23430048,
CC ECO:0000269|PubMed:24484953}.
CC -!- INTERACTION:
CC O22131; P59467: LBD23; NbExp=3; IntAct=EBI-15212656, EBI-15193845;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19088331}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, leaves and flowers
CC (PubMed:12068116, PubMed:24484953). Expressed in vascular tissues of
CC hypocotyls, leaves, roots, developing floral organs and siliques
CC (PubMed:19088331). {ECO:0000269|PubMed:12068116,
CC ECO:0000269|PubMed:19088331, ECO:0000269|PubMed:24484953}.
CC -!- DEVELOPMENTAL STAGE: During lateral root formation, expressed in the
CC lateral root primordia, and the developing, emerged, and mature lateral
CC roots. {ECO:0000269|PubMed:19717544}.
CC -!- INDUCTION: By auxin. {ECO:0000269|PubMed:15659631,
CC ECO:0000269|PubMed:19717544, ECO:0000269|PubMed:23749813}.
CC -!- DISRUPTION PHENOTYPE: Reduced number of lateral roots.
CC {ECO:0000269|PubMed:19717544}.
CC -!- MISCELLANEOUS: Plants over-expressing LBD18 have a dwarf and bushy
CC phenotype, with short petioles, curled downward leaves, ectopic shoots
CC from the adaxial side of cotyledons, shrunken root tips and
CC disorganized columella cells. {ECO:0000269|PubMed:19088331}.
CC -!- SIMILARITY: Belongs to the LOB domain-containing protein family.
CC {ECO:0000305}.
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DR EMBL; AF447891; AAL38036.1; -; mRNA.
DR EMBL; AB473853; BAH10564.1; -; mRNA.
DR EMBL; AC002387; AAB82622.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC10551.1; -; Genomic_DNA.
DR EMBL; BT025716; ABF82619.1; -; mRNA.
DR PIR; C84890; C84890.
DR RefSeq; NP_850436.1; NM_180105.2.
DR AlphaFoldDB; O22131; -.
DR SMR; O22131; -.
DR BioGRID; 4486; 6.
DR IntAct; O22131; 2.
DR STRING; 3702.AT2G45420.1; -.
DR PaxDb; O22131; -.
DR PRIDE; O22131; -.
DR ProteomicsDB; 237150; -.
DR EnsemblPlants; AT2G45420.1; AT2G45420.1; AT2G45420.
DR GeneID; 819150; -.
DR Gramene; AT2G45420.1; AT2G45420.1; AT2G45420.
DR KEGG; ath:AT2G45420; -.
DR Araport; AT2G45420; -.
DR TAIR; locus:2050951; AT2G45420.
DR eggNOG; ENOG502QR68; Eukaryota.
DR HOGENOM; CLU_058353_3_5_1; -.
DR InParanoid; O22131; -.
DR OMA; DPPTSWA; -.
DR OrthoDB; 1304135at2759; -.
DR PhylomeDB; O22131; -.
DR PRO; PR:O22131; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22131; baseline and differential.
DR Genevisible; O22131; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:1990110; P:callus formation; IMP:TAIR.
DR GO; GO:0009755; P:hormone-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0010311; P:lateral root formation; IMP:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0010089; P:xylem development; IMP:TAIR.
DR InterPro; IPR004883; LOB.
DR InterPro; IPR017394; LOB_18/20.
DR Pfam; PF03195; LOB; 1.
DR PIRSF; PIRSF038127; UCP038127_LOB; 1.
DR PROSITE; PS50891; LOB; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..262
FT /note="LOB domain-containing protein 18"
FT /id="PRO_0000132269"
FT DOMAIN 36..138
FT /note="LOB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00291"
FT REGION 223..262
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 109
FT /note="P->L: Loss of function in lateral root formation.
FT Abolishes DNA-binding activity. Abolishes interaction with
FT GIP1."
FT /evidence="ECO:0000269|PubMed:23430048,
FT ECO:0000269|PubMed:24484953"
SQ SEQUENCE 262 AA; 27219 MW; 4DE2F64EC148AEF5 CRC64;
MSGGGNTITA VGGGGGGCGG GGSSGGGGSS GGGGGGPCGA CKFLRRKCVP GCIFAPYFDS
EQGSAYFAAV HKVFGASNVS KLLLHIPVHR RSDAVVTICY EAQARIRDPI YGCVAHIFAL
QQQVVNLQAE VSYLQAHLAS LELPQPQTRP QPMPQPQPLF FTPPPPLAIT DLPASVSPLP
STYDLASIFD QTTSSSAWAT QQRRFIDPRH QYGVSSSSSS VAVGLGGENS HDLQALAHEL
LHRQGSPPPA ATDHSPSRTM SR
