LBH_MOUSE
ID LBH_MOUSE Reviewed; 105 AA.
AC Q9CX60; Q99MQ2;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Protein LBH;
DE AltName: Full=Limb bud and heart-expressed protein;
GN Name=Lbh;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=Swiss Webster / NIH; TISSUE=Embryo;
RX PubMed=11336496; DOI=10.1006/dbio.2001.0225;
RA Briegel K.J., Joyner A.L.;
RT "Identification and characterization of Lbh, a novel conserved nuclear
RT protein expressed during early limb and heart development.";
RL Dev. Biol. 233:291-304(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Wolffian duct;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Brain, and Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-63, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic brain;
RX PubMed=15345747; DOI=10.1074/mcp.m400085-mcp200;
RA Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.;
RT "Phosphoproteomic analysis of the developing mouse brain.";
RL Mol. Cell. Proteomics 3:1093-1101(2004).
RN [5]
RP FUNCTION.
RX PubMed=15958514; DOI=10.1242/dev.01887;
RA Briegel K.J., Baldwin H.S., Epstein J.A., Joyner A.L.;
RT "Congenital heart disease reminiscent of partial trisomy 2p syndrome in
RT mice transgenic for the transcription factor Lbh.";
RL Development 132:3305-3316(2005).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-63, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Heart, Kidney, Lung, Pancreas, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Modulates the activity of key transcription factors involved
CC in cardiogenesis. {ECO:0000269|PubMed:11336496,
CC ECO:0000269|PubMed:15958514}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11336496}. Cytoplasm
CC {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed at highest levels in heart, and at lower
CC levels in brain, spleen, lung, liver and kidney.
CC {ECO:0000269|PubMed:11336496}.
CC -!- DEVELOPMENTAL STAGE: First detected at 7.5 dpc in the mesendoderm that
CC forms the anterior gut and the heart. At midgestation, expressed in the
CC limb bud ectoderm and other specialized epithelia, as well as in the
CC branchial arches and some neural crest derivatives. In the heart,
CC expression initiates in the myocardial plate at the presomitic stage,
CC with highest levels in the anterior myocardium. During heart chamber
CC formation, expressed transiently in the right ventricle,
CC atrioventricular canal and inflow tract. In the limb bud, expression is
CC restricted to the ventral ectodermal compartment and the apical
CC ectodermal ridge. {ECO:0000269|PubMed:11336496}.
CC -!- MISCELLANEOUS: Mice overexpressing Lbh in heart exhibit severe cardiac
CC abnormalities and dysfunction.
CC -!- SIMILARITY: Belongs to the LBH family. {ECO:0000305}.
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DR EMBL; AF317517; AAK38204.1; -; mRNA.
DR EMBL; AK020110; BAB31998.1; -; mRNA.
DR EMBL; BC026827; AAH26827.1; -; mRNA.
DR EMBL; BC052470; AAH52470.1; -; mRNA.
DR CCDS; CCDS28964.1; -.
DR RefSeq; NP_084275.3; NM_029999.4.
DR AlphaFoldDB; Q9CX60; -.
DR BioGRID; 218999; 1.
DR STRING; 10090.ENSMUSP00000024857; -.
DR iPTMnet; Q9CX60; -.
DR PhosphoSitePlus; Q9CX60; -.
DR EPD; Q9CX60; -.
DR jPOST; Q9CX60; -.
DR MaxQB; Q9CX60; -.
DR PaxDb; Q9CX60; -.
DR PeptideAtlas; Q9CX60; -.
DR PRIDE; Q9CX60; -.
DR ProteomicsDB; 264980; -.
DR Antibodypedia; 28981; 91 antibodies from 16 providers.
DR DNASU; 77889; -.
DR Ensembl; ENSMUST00000024857; ENSMUSP00000024857; ENSMUSG00000024063.
DR GeneID; 77889; -.
DR KEGG; mmu:77889; -.
DR UCSC; uc008dng.2; mouse.
DR CTD; 81606; -.
DR MGI; MGI:1925139; Lbh.
DR VEuPathDB; HostDB:ENSMUSG00000024063; -.
DR eggNOG; ENOG502S1QG; Eukaryota.
DR GeneTree; ENSGT00390000009189; -.
DR HOGENOM; CLU_161464_0_0_1; -.
DR InParanoid; Q9CX60; -.
DR OMA; CPLPCSD; -.
DR OrthoDB; 1541774at2759; -.
DR PhylomeDB; Q9CX60; -.
DR TreeFam; TF332770; -.
DR BioGRID-ORCS; 77889; 3 hits in 73 CRISPR screens.
DR ChiTaRS; Lbh; mouse.
DR PRO; PR:Q9CX60; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q9CX60; protein.
DR Bgee; ENSMUSG00000024063; Expressed in ascending aorta and 229 other tissues.
DR ExpressionAtlas; Q9CX60; baseline and differential.
DR Genevisible; Q9CX60; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:CAFA.
DR GO; GO:0032991; C:protein-containing complex; ISS:UniProtKB.
DR GO; GO:0030879; P:mammary gland development; IMP:CAFA.
DR GO; GO:0060644; P:mammary gland epithelial cell differentiation; IMP:CAFA.
DR GO; GO:0033147; P:negative regulation of intracellular estrogen receptor signaling pathway; IMP:CAFA.
DR GO; GO:2000737; P:negative regulation of stem cell differentiation; IMP:CAFA.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:2000103; P:positive regulation of mammary stem cell proliferation; IMP:CAFA.
DR GO; GO:1904677; P:positive regulation of somatic stem cell division; IMP:CAFA.
DR GO; GO:1904674; P:positive regulation of somatic stem cell population maintenance; IMP:CAFA.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:MGI.
DR GO; GO:0010468; P:regulation of gene expression; IMP:CAFA.
DR GO; GO:0043408; P:regulation of MAPK cascade; ISS:UniProtKB.
DR DisProt; DP00661; -.
DR InterPro; IPR013294; LBH.
DR InterPro; IPR038990; LBH_dom.
DR InterPro; IPR042945; LBH_dom_prot.
DR PANTHER; PTHR14987; PTHR14987; 1.
DR Pfam; PF15317; Lbh; 1.
DR PIRSF; PIRSF008130; LBH; 1.
DR PRINTS; PR01881; LBHPROTEIN.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..105
FT /note="Protein LBH"
FT /id="PRO_0000324803"
FT DOMAIN 18..103
FT /note="LBH"
FT /evidence="ECO:0000255"
FT REGION 68..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 72..86
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 63
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:15345747,
FT ECO:0007744|PubMed:21183079"
FT CONFLICT 98
FT /note="T -> A (in Ref. 1; AAK38204)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 105 AA; 12138 MW; 0F794B2BAF966EEA CRC64;
MSVYFPIHCS DYLRSAEMTE VMMNAPSMEE IGLSPRKDGL SYQIFPDPSD FDRCCKLKDR
LPSIVVEPTE GEVESGELRW PPEEFLVQED EQDNCEETTN EKKDQ
