LBX1_MOUSE
ID LBX1_MOUSE Reviewed; 282 AA.
AC P52955; Q3UN09; Q80XA3;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2006, sequence version 2.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Transcription factor LBX1;
DE AltName: Full=Ladybird homeobox protein homolog 1;
GN Name=Lbx1; Synonyms=Lbx1h;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=BALB/cJ;
RX PubMed=8645601; DOI=10.1016/0925-4773(95)00450-5;
RA Jagla K., Dolle P., Mattei M.-G., Jagla T., Schuhbaur B., Dretzen G.,
RA Bellard F., Bellard M.;
RT "Mouse Lbx1 and human LBX1 define a novel mammalian homeobox gene family
RT related to the Drosophila lady bird genes.";
RL Mech. Dev. 53:345-356(1995).
RN [2]
RP SEQUENCE REVISION.
RA Jagla K.;
RL Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=ICR;
RA Kurose T., Endo T.;
RT "Mouse homeobox gene Lbx1 in myogenesis.";
RL Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 50-282.
RC STRAIN=C57BL/6J; TISSUE=Medulla oblongata;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=10603359; DOI=10.1242/dev.127.2.437;
RA Brohmann H., Jagla K., Birchmeier C.;
RT "The role of Lbx1 in migration of muscle precursor cells.";
RL Development 127:437-445(2000).
RN [7]
RP FUNCTION.
RX PubMed=12062039; DOI=10.1016/s0896-6273(02)00689-x;
RA Muller T., Brohmann H., Pierani A., Heppenstall P.A., Lewin G.R.,
RA Jessell T.M., Birchmeier C.;
RT "The homeodomain factor lbx1 distinguishes two major programs of neuronal
RT differentiation in the dorsal spinal cord.";
RL Neuron 34:551-562(2002).
RN [8]
RP INTERACTION WITH SKOR1.
RX PubMed=15528197; DOI=10.1074/jbc.m411652200;
RA Mizuhara E., Nakatani T., Minaki Y., Sakamoto Y., Ono Y.;
RT "Corl1, a novel neuronal lineage-specific transcriptional corepressor for
RT the homeodomain transcription factor Lbx1.";
RL J. Biol. Chem. 280:3645-3655(2005).
RN [9]
RP FUNCTION.
RX PubMed=16234809; DOI=10.1038/nn1569;
RA Cheng L., Samad O.A., Xu Y., Mizuguchi R., Luo P., Shirasawa S.,
RA Goulding M., Ma Q.;
RT "Lbx1 and Tlx3 are opposing switches in determining GABAergic versus
RT glutamatergic transmitter phenotypes.";
RL Nat. Neurosci. 8:1510-1515(2005).
CC -!- FUNCTION: Transcription factor required for the development of
CC GABAergic interneurons in the dorsal horn of the spinal cord and
CC migration and further development of hypaxial muscle precursor cells
CC for limb muscles, diaphragm and hypoglossal cord.
CC {ECO:0000269|PubMed:10603359, ECO:0000269|PubMed:12062039,
CC ECO:0000269|PubMed:16234809}.
CC -!- SUBUNIT: Interacts with SKOR1 which acts as a transcriptional
CC corepressor. {ECO:0000269|PubMed:15528197}.
CC -!- INTERACTION:
CC P52955; Q8BX46: Skor1; NbExp=2; IntAct=EBI-604594, EBI-604451;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in the dorsal part of the spinal cord and
CC hindbrain and in presumptive myogenic cells in lateral regions of
CC differentiating somites. {ECO:0000269|PubMed:8645601}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the developing central nervous system
CC from 10.5 dpc to 16.5 dpc. Expressed in presumptive myogenic cells from
CC 9.5 dpc until 16.5 dpc with highest levels at 10.5-11.5 dpc.
CC {ECO:0000269|PubMed:8645601}.
CC -!- DISRUPTION PHENOTYPE: Death at birth. Mice fail to expand their lungs
CC and do not move their abnormally thin limbs.
CC {ECO:0000269|PubMed:10603359}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE25939.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X90829; CAA62343.1; -; mRNA.
DR EMBL; AB108497; BAC75634.1; -; mRNA.
DR EMBL; BC119177; AAI19178.1; -; mRNA.
DR EMBL; BC120586; AAI20587.1; -; mRNA.
DR EMBL; AK144562; BAE25939.1; ALT_INIT; mRNA.
DR CCDS; CCDS29859.1; -.
DR RefSeq; NP_034821.2; NM_010691.5.
DR RefSeq; XP_006526785.1; XM_006526722.3.
DR AlphaFoldDB; P52955; -.
DR SMR; P52955; -.
DR BioGRID; 201117; 2.
DR IntAct; P52955; 2.
DR STRING; 10090.ENSMUSP00000096997; -.
DR iPTMnet; P52955; -.
DR PhosphoSitePlus; P52955; -.
DR PaxDb; P52955; -.
DR PRIDE; P52955; -.
DR ProteomicsDB; 263702; -.
DR Antibodypedia; 911; 174 antibodies from 27 providers.
DR DNASU; 16814; -.
DR Ensembl; ENSMUST00000099401; ENSMUSP00000096997; ENSMUSG00000025216.
DR GeneID; 16814; -.
DR KEGG; mmu:16814; -.
DR UCSC; uc008hqv.1; mouse.
DR CTD; 10660; -.
DR MGI; MGI:104867; Lbx1.
DR VEuPathDB; HostDB:ENSMUSG00000025216; -.
DR eggNOG; KOG0488; Eukaryota.
DR GeneTree; ENSGT00940000161756; -.
DR HOGENOM; CLU_086390_0_0_1; -.
DR InParanoid; P52955; -.
DR OMA; ADKHSPG; -.
DR OrthoDB; 1472248at2759; -.
DR PhylomeDB; P52955; -.
DR TreeFam; TF325047; -.
DR BioGRID-ORCS; 16814; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Lbx1; mouse.
DR PRO; PR:P52955; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; P52955; protein.
DR Bgee; ENSMUSG00000025216; Expressed in triceps brachii and 62 other tissues.
DR Genevisible; P52955; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005667; C:transcription regulator complex; IDA:MGI.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0008283; P:cell population proliferation; IMP:MGI.
DR GO; GO:1905962; P:glutamatergic neuron differentiation; IMP:MGI.
DR GO; GO:0001947; P:heart looping; IMP:MGI.
DR GO; GO:0007517; P:muscle organ development; IEA:UniProtKB-KW.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IMP:MGI.
DR GO; GO:0120007; P:negative regulation of glutamatergic neuron differentiation; IMP:MGI.
DR GO; GO:0048663; P:neuron fate commitment; IMP:MGI.
DR GO; GO:0048664; P:neuron fate determination; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:MGI.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:MGI.
DR GO; GO:0021522; P:spinal cord motor neuron differentiation; IGI:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR000047; HTH_motif.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; DNA-binding; Homeobox; Myogenesis;
KW Neurogenesis; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..282
FT /note="Transcription factor LBX1"
FT /id="PRO_0000049167"
FT DNA_BIND 125..184
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 210..282
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..21
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 268..282
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 33..72
FT /note="LTPFSIEDILNKPSVRRSYSLCGAAHLLAAADKHAPGGLP -> YAVQHRGH
FT PQQAVRAEKLLAVWGGAPAGGRGQARAGRLA (in Ref. 1; CAA62343)"
FT /evidence="ECO:0000305"
FT CONFLICT 225
FT /note="Missing (in Ref. 1; CAA62343)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 282 AA; 30262 MW; 3A4A80CD47B0228C CRC64;
MTSKEDGKAA PGEERRRSPL DHLPPPANSN KPLTPFSIED ILNKPSVRRS YSLCGAAHLL
AAADKHAPGG LPLAGRALLS QTSPLCALEE LASKTFKGLE VSVLQAAEGR DGMTIFGQRQ
TPKKRRKSRT AFTNHQIYEL EKRFLYQKYL SPADRDQIAQ QLGLTNAQVI TWFQNRRAKL
KRDLEEMKAD VESAKKLGPS GQMDIVALAE LEQNSEASGG GGGGGCGRAK SRPGSPALPP
GAPQAPGGGP LQLSPASPLT DQRASSQDCS EDEEDEEIDV DD
