LC7L2_MOUSE
ID LC7L2_MOUSE Reviewed; 392 AA.
AC Q7TNC4; Q99LM5; Q99PC3;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Putative RNA-binding protein Luc7-like 2;
DE AltName: Full=CGI-74 homolog;
GN Name=Luc7l2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=NMRI; TISSUE=Liver;
RA Utsch B., Albers N., Wickert L., Schubert R., Bidlingmaier F., Ludwig M.;
RT "CGI-74 homolog in the mouse.";
RL Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND NUCLEOTIDE
RP SEQUENCE [LARGE SCALE MRNA] OF 222-392 (ISOFORM 3).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 135-311 (ISOFORMS 1/2/3/4), INTERACTION WITH
RP SCNM1, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING, AND TISSUE SPECIFICITY.
RC TISSUE=Embryo;
RX PubMed=17656373; DOI=10.1093/hmg/ddm206;
RA Howell V.M., Jones J.M., Bergren S.K., Li L., Billi A.C., Avenarius M.R.,
RA Meisler M.H.;
RT "Evidence for a direct role of the disease modifier SCNM1 in splicing.";
RL Hum. Mol. Genet. 16:2506-2516(2007).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=19131326; DOI=10.1074/mcp.m800451-mcp200;
RA Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.;
RT "Large scale localization of protein phosphorylation by use of electron
RT capture dissociation mass spectrometry.";
RL Mol. Cell. Proteomics 8:904-912(2009).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, Kidney, Liver, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May bind to RNA via its Arg/Ser-rich domain.
CC -!- SUBUNIT: Interacts with SCNM1. {ECO:0000269|PubMed:17656373}.
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000269|PubMed:17656373}.
CC Nucleus, nucleoplasm {ECO:0000269|PubMed:17656373}. Note=Colocalizes
CC with SCNM1 and SNRNP70 in nuclear speckles.
CC {ECO:0000269|PubMed:17656373}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q7TNC4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7TNC4-2; Sequence=VSP_010218, VSP_010219, VSP_010220;
CC Name=3;
CC IsoId=Q7TNC4-3; Sequence=VSP_010219, VSP_010220;
CC Name=4;
CC IsoId=Q7TNC4-4; Sequence=VSP_010218;
CC -!- TISSUE SPECIFICITY: All isoforms are expressed in brain, kidney, heart,
CC thymus, stomach, skeletal muscle, testis and spinal cord.
CC {ECO:0000269|PubMed:17656373}.
CC -!- SIMILARITY: Belongs to the Luc7 family. {ECO:0000305}.
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DR EMBL; AF318301; AAK01182.1; -; mRNA.
DR EMBL; BC002314; AAH02314.1; -; mRNA.
DR EMBL; BC056354; AAH56354.1; -; mRNA.
DR EMBL; BC056383; AAH56383.1; -; mRNA.
DR EMBL; BC056970; AAH56970.1; -; mRNA.
DR CCDS; CCDS20015.1; -. [Q7TNC4-1]
DR CCDS; CCDS51751.1; -. [Q7TNC4-2]
DR RefSeq; NP_001164320.1; NM_001170849.2. [Q7TNC4-2]
DR RefSeq; NP_619621.2; NM_138680.3. [Q7TNC4-1]
DR AlphaFoldDB; Q7TNC4; -.
DR SMR; Q7TNC4; -.
DR BioGRID; 228673; 16.
DR IntAct; Q7TNC4; 2.
DR STRING; 10090.ENSMUSP00000055254; -.
DR iPTMnet; Q7TNC4; -.
DR PhosphoSitePlus; Q7TNC4; -.
DR EPD; Q7TNC4; -.
DR MaxQB; Q7TNC4; -.
DR PaxDb; Q7TNC4; -.
DR PeptideAtlas; Q7TNC4; -.
DR PRIDE; Q7TNC4; -.
DR ProteomicsDB; 252455; -. [Q7TNC4-1]
DR ProteomicsDB; 252456; -. [Q7TNC4-2]
DR ProteomicsDB; 252457; -. [Q7TNC4-3]
DR ProteomicsDB; 252458; -. [Q7TNC4-4]
DR Antibodypedia; 53629; 143 antibodies from 26 providers.
DR DNASU; 192196; -.
DR Ensembl; ENSMUST00000057692; ENSMUSP00000055254; ENSMUSG00000029823. [Q7TNC4-1]
DR Ensembl; ENSMUST00000161538; ENSMUSP00000124010; ENSMUSG00000029823. [Q7TNC4-2]
DR GeneID; 192196; -.
DR KEGG; mmu:192196; -.
DR UCSC; uc009bkl.2; mouse. [Q7TNC4-2]
DR UCSC; uc009bkm.2; mouse. [Q7TNC4-1]
DR CTD; 51631; -.
DR MGI; MGI:2183260; Luc7l2.
DR VEuPathDB; HostDB:ENSMUSG00000029823; -.
DR eggNOG; KOG0796; Eukaryota.
DR GeneTree; ENSGT00950000183213; -.
DR HOGENOM; CLU_030397_3_0_1; -.
DR InParanoid; Q7TNC4; -.
DR OMA; AHKADYE; -.
DR OrthoDB; 1499212at2759; -.
DR PhylomeDB; Q7TNC4; -.
DR TreeFam; TF317607; -.
DR BioGRID-ORCS; 192196; 2 hits in 70 CRISPR screens.
DR ChiTaRS; Luc7l2; mouse.
DR PRO; PR:Q7TNC4; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q7TNC4; protein.
DR Bgee; ENSMUSG00000029823; Expressed in mammillary body and 255 other tissues.
DR ExpressionAtlas; Q7TNC4; baseline and differential.
DR Genevisible; Q7TNC4; MM.
DR GO; GO:0016607; C:nuclear speck; IDA:UniProtKB.
DR GO; GO:0005685; C:U1 snRNP; IBA:GO_Central.
DR GO; GO:0071004; C:U2-type prespliceosome; IBA:GO_Central.
DR GO; GO:0019899; F:enzyme binding; ISO:MGI.
DR GO; GO:0003729; F:mRNA binding; IBA:GO_Central.
DR GO; GO:0006376; P:mRNA splice site selection; IBA:GO_Central.
DR InterPro; IPR004882; Luc7-rel.
DR PANTHER; PTHR12375; PTHR12375; 1.
DR Pfam; PF03194; LUC7; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Hydroxylation; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..392
FT /note="Putative RNA-binding protein Luc7-like 2"
FT /id="PRO_0000187283"
FT REGION 235..392
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 102..177
FT /evidence="ECO:0000255"
FT COMPBIAS 235..261
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 262..328
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 329..392
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 18
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 266
FT /note="5-hydroxylysine; by JMJD6"
FT /evidence="ECO:0000250"
FT MOD_RES 269
FT /note="5-hydroxylysine; by JMJD6"
FT /evidence="ECO:0000250"
FT VAR_SEQ 260..269
FT /note="Missing (in isoform 2 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_010218"
FT VAR_SEQ 335
FT /note="S -> Y (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_010219"
FT VAR_SEQ 336..392
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_010220"
FT CONFLICT 147
FT /note="G -> E (in Ref. 2; AAH56383)"
FT /evidence="ECO:0000305"
FT CONFLICT 245
FT /note="K -> L (in Ref. 1; AAK01182)"
FT /evidence="ECO:0000305"
FT CONFLICT 259
FT /note="R -> W (in Ref. 1; AAK01182)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 392 AA; 46583 MW; 1A5202B1F537A7CC CRC64;
MSAQAQMRAM LDQLMGTSRD GDTTRQRIKF SDDRVCKSHL LNCCPHDVLS GTRMDLGECL
KVHDLALRAD YEIASKEQDF FFELDAMDHL QSFIADCDRR TEVSKKRLAE TQEEISAEVA
AKAERVHELN EEIGKLLAKV EQLGAEGNVE ESQKVMDEVE KARAKKREAE EVYRNSMPAS
SFQQQKLRVC EVCSAYLGLH DNDRRLADHF GGKLHLGFIE IREKLEELKR VVAEKQEKRN
QERLKRREER EREEREKLRR SRSHSKNPKR SRSREHRRHR SRSMSRERKR RTRSKSREKR
HRHRSRSSSR SRSRSHQRSR HSSRDRSRER SKRRSSKERF RDQDLASRDR DRSSRDRSPR
DRDRKDKKRS YESANGRSED RRSSEEREAG EI
