LCAT1_ARATH
ID LCAT1_ARATH Reviewed; 432 AA.
AC Q9FZI8;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Lecithin-cholesterol acyltransferase-like 1;
DE EC=2.3.1.-;
GN Name=LCAT1; OrderedLocusNames=At1g27480; ORFNames=F17L21.27, F17L21.28;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=15355352; DOI=10.1111/j.1432-1033.2004.04317.x;
RA Noiriel A., Benveniste P., Banas A., Stymne S., Bouvier-Nave P.;
RT "Expression in yeast of a novel phospholipase A1 cDNA from Arabidopsis
RT thaliana.";
RL Eur. J. Biochem. 271:3752-3764(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family.
CC {ECO:0000305}.
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DR EMBL; AH013087; AAQ14348.1; -; Genomic_DNA.
DR EMBL; AY443040; AAR31109.1; -; mRNA.
DR EMBL; AC004557; AAF99739.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30837.1; -; Genomic_DNA.
DR EMBL; AF367326; AAK32913.1; -; mRNA.
DR EMBL; AY133614; AAM91444.1; -; mRNA.
DR EMBL; AY087433; AAM64980.1; -; mRNA.
DR RefSeq; NP_564286.1; NM_102512.3.
DR AlphaFoldDB; Q9FZI8; -.
DR SMR; Q9FZI8; -.
DR STRING; 3702.AT1G27480.1; -.
DR ESTHER; arath-LCAT1; PC-sterol_acyltransferase.
DR PaxDb; Q9FZI8; -.
DR PRIDE; Q9FZI8; -.
DR ProteomicsDB; 237127; -.
DR EnsemblPlants; AT1G27480.1; AT1G27480.1; AT1G27480.
DR GeneID; 839639; -.
DR Gramene; AT1G27480.1; AT1G27480.1; AT1G27480.
DR KEGG; ath:AT1G27480; -.
DR Araport; AT1G27480; -.
DR TAIR; locus:2015924; AT1G27480.
DR eggNOG; KOG2369; Eukaryota.
DR HOGENOM; CLU_037070_2_1_1; -.
DR InParanoid; Q9FZI8; -.
DR OMA; PNENKQF; -.
DR OrthoDB; 828056at2759; -.
DR PhylomeDB; Q9FZI8; -.
DR BioCyc; ARA:AT1G27480-MON; -.
DR PRO; PR:Q9FZI8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9FZI8; baseline and differential.
DR Genevisible; Q9FZI8; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0008374; F:O-acyltransferase activity; IEA:InterPro.
DR GO; GO:0006629; P:lipid metabolic process; IBA:GO_Central.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR003386; LACT/PDAT_acylTrfase.
DR Pfam; PF02450; LCAT; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Membrane; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..432
FT /note="Lecithin-cholesterol acyltransferase-like 1"
FT /id="PRO_0000398819"
FT TRANSMEM 7..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ACT_SITE 209
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000255"
FT ACT_SITE 374
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 400
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
SQ SEQUENCE 432 AA; 48308 MW; 4ACD9608C6785078 CRC64;
MKKISSHYSV VIAILVVVTM TSMCQAVGSN VYPLILVPGN GGNQLEVRLD REYKPSSVWC
SSWLYPIHKK SGGWFRLWFD AAVLLSPFTR CFSDRMMLYY DPDLDDYQNA PGVQTRVPHF
GSTKSLLYLD PRLRDATSYM EHLVKALEKK CGYVNDQTIL GAPYDFRYGL AASGHPSRVA
SQFLQDLKQL VEKTSSENEG KPVILLSHSL GGLFVLHFLN RTTPSWRRKY IKHFVALAAP
WGGTISQMKT FASGNTLGVP LVNPLLVRRH QRTSESNQWL LPSTKVFHDR TKPLVVTPQV
NYTAYEMDRF FADIGFSQGV VPYKTRVLPL TEELMTPGVP VTCIYGRGVD TPEVLMYGKG
GFDKQPEIKY GDGDGTVNLA SLAALKVDSL NTVEIDGVSH TSILKDEIAL KEIMKQISII
NYELANVNAV NE
