LCAT4_ARATH
ID LCAT4_ARATH Reviewed; 535 AA.
AC Q71N54; O81867;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Lecithin-cholesterol acyltransferase-like 4;
DE EC=2.3.1.-;
GN Name=LCAT4; OrderedLocusNames=At4g19860; ORFNames=T16H5.220;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=15355352; DOI=10.1111/j.1432-1033.2004.04317.x;
RA Noiriel A., Benveniste P., Banas A., Stymne S., Bouvier-Nave P.;
RT "Expression in yeast of a novel phospholipase A1 cDNA from Arabidopsis
RT thaliana.";
RL Eur. J. Biochem. 271:3752-3764(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA19703.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB78988.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF421149; AAQ04052.1; -; mRNA.
DR EMBL; AL024486; CAA19703.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161551; CAB78988.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE84235.1; -; Genomic_DNA.
DR EMBL; BT022028; AAY25440.1; -; mRNA.
DR PIR; T04767; T04767.
DR RefSeq; NP_193721.2; NM_118106.4.
DR AlphaFoldDB; Q71N54; -.
DR SMR; Q71N54; -.
DR STRING; 3702.AT4G19860.1; -.
DR ESTHER; arath-LCAT4; PC-sterol_acyltransferase.
DR iPTMnet; Q71N54; -.
DR PaxDb; Q71N54; -.
DR PRIDE; Q71N54; -.
DR ProteomicsDB; 237058; -.
DR EnsemblPlants; AT4G19860.1; AT4G19860.1; AT4G19860.
DR GeneID; 827730; -.
DR Gramene; AT4G19860.1; AT4G19860.1; AT4G19860.
DR KEGG; ath:AT4G19860; -.
DR Araport; AT4G19860; -.
DR TAIR; locus:2133975; AT4G19860.
DR eggNOG; KOG2369; Eukaryota.
DR HOGENOM; CLU_035096_0_0_1; -.
DR InParanoid; Q71N54; -.
DR OMA; HVELNAM; -.
DR OrthoDB; 828056at2759; -.
DR PhylomeDB; Q71N54; -.
DR BioCyc; ARA:AT4G19860-MON; -.
DR PRO; PR:Q71N54; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q71N54; baseline and differential.
DR Genevisible; Q71N54; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:TAIR.
DR GO; GO:0008374; F:O-acyltransferase activity; IEA:InterPro.
DR GO; GO:0004620; F:phospholipase activity; IDA:TAIR.
DR GO; GO:0006629; P:lipid metabolic process; IBA:GO_Central.
DR GO; GO:0009395; P:phospholipid catabolic process; IDA:TAIR.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR003386; LACT/PDAT_acylTrfase.
DR Pfam; PF02450; LCAT; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 1: Evidence at protein level;
KW Acetylation; Acyltransferase; Reference proteome; Transferase.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 2..535
FT /note="Lecithin-cholesterol acyltransferase-like 4"
FT /id="PRO_0000398822"
FT REGION 488..507
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 182
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000255"
FT ACT_SITE 391
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 416
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:22223895"
SQ SEQUENCE 535 AA; 60431 MW; A60A460DB07BD305 CRC64;
MSLLLEEIIR SVEALLKLRN RNQEPYVDPN LNPVLLVPGI AGSILNAVDH ENGNEERVWV
RIFGADHEFR TKMWSRFDPS TGKTISLDPK TSIVVPQDRA GLHAIDVLDP DMIVGRESVY
YFHEMIVEMI GWGFEEGKTL FGFGYDFRQS NRLQETLDQF AKKLETVYKA SGEKKINVIS
HSMGGLLVKC FMGLHSDIFE KYVQNWIAIA APFRGAPGYI TSTLLNGMSF VNGWEQNFFV
SKWSMHQLLI ECPSIYELMC CPYFKWELPP VLELWREKES NDGVGTSYVV LESYCSLESL
EVFTKSLSNN TADYCGESID LPFNWKIMEW AHKTKQVLAS AKLPPKVKFY NIYGTNLETP
HSVCYGNEKM PVKDLTNLRY FQPTYICVDG DGTVPMESAM ADGLEAVARV GVPGEHRGIL
NDHRVFRMLK KWLNVGEPDP FYNPVNDYVI LPTTYEFEKF HENGLEVASV KESWDIISDD
NNIGTTGSTV NSISVSQPGD DQNPQAEARA TLTVQPQSDG RQHVELNAVS VSVDA
