ID   LCA_LYCCL               Reviewed;         755 AA.
AC   A0A125SXN3;
DT   07-NOV-2018, integrated into UniProtKB/Swiss-Prot.
DT   13-APR-2016, sequence version 1.
DT   25-MAY-2022, entry version 16.
DE   RecName: Full=Cycloartenol synthase LCA {ECO:0000305|PubMed:26663356};
DE            EC=5.4.99.8 {ECO:0000269|PubMed:26663356};
GN   Name=LCA {ECO:0000303|PubMed:26663356};
OS   Lycopodium clavatum (Stag's-horn clubmoss).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Lycopodiopsida; Lycopodiales; Lycopodiaceae; Lycopodioideae; Lycopodium.
OX   NCBI_TaxID=3252;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX   PubMed=26663356; DOI=10.1002/cbic.201500663;
RA   Araki T., Saga Y., Marugami M., Otaka J., Araya H., Saito K., Yamazaki M.,
RA   Suzuki H., Kushiro T.;
RT   "Onocerin biosynthesis requires two highly dedicated triterpene cyclases in
RT   a fern Lycopodium clavatum.";
RL   ChemBioChem 17:288-290(2016).
CC   -!- FUNCTION: Oxidosqualene cyclase involved in the biosynthesis of
CC       cycloartenol. {ECO:0000269|PubMed:26663356}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = cycloartenol; Xref=Rhea:RHEA:21308,
CC         ChEBI:CHEBI:15441, ChEBI:CHEBI:17030; EC=5.4.99.8;
CC         Evidence={ECO:0000269|PubMed:26663356};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC       {ECO:0000269|PubMed:26663356}.
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; LC053637; BAU46473.1; -; mRNA.
DR   UniPathway; UPA00213; -.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0016871; F:cycloartenol synthase activity; IDA:UniProtKB.
DR   GO; GO:0019745; P:pentacyclic triterpenoid biosynthetic process; IDA:UniProtKB.
DR   GO; GO:0010686; P:tetracyclic triterpenoid biosynthetic process; IDA:UniProtKB.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Repeat.
FT   CHAIN           1..755
FT                   /note="Cycloartenol synthase LCA"
FT                   /id="PRO_0000445703"
FT   REPEAT          97..142
FT                   /note="PFTB 1"
FT                   /evidence="ECO:0000255"
FT   REPEAT          147..188
FT                   /note="PFTB 2"
FT                   /evidence="ECO:0000255"
FT   REPEAT          390..431
FT                   /note="PFTB 3"
FT                   /evidence="ECO:0000255"
FT   REPEAT          511..556
FT                   /note="PFTB 4"
FT                   /evidence="ECO:0000255"
FT   REPEAT          588..628
FT                   /note="PFTB 5"
FT                   /evidence="ECO:0000255"
FT   REPEAT          637..678
FT                   /note="PFTB 6"
FT                   /evidence="ECO:0000255"
FT   REPEAT          699..740
FT                   /note="PFTB 7"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        482
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
FT   SITE            415
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
FT   SITE            471
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
FT   SITE            609
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   755 AA;  85663 MW;  3F5C5D54DA1EB133 CRC64;
     MWSLKIAEGG GPWMSSLNDH VGRQIWQYDD NGGTPQERSA IDALQAQFTR NRHDVRHSSD
     SIMRFQFSKD NPLPPLPLQV KVKGHEQLTR DIVEKTLRRA LRFYSTIQAN DGHWPGDYGG
     PMFLMPGLVI TLYITGALRV ILSEQHQLEM CRYLYNHQNN DGGWGLHIEG HSTMFGTILT
     YVTLRLLNQG SGAHAAMEKA RAWILDHGGA TAAPSWGKFW LAALGIFDWS GVDPLPPEMW
     LLPYILPVYP GRMWCHCRMV YLPMSYIYGR RYSSPLTKLT SDIRNEIYVV PYEEVNWDSA
     RNDCAREDLY YPHPFLQDLL WATLHKCVEP ILMHWPGSLL RQRALAMVIE HIHYKDENTR
     YVCIGPVNKV INMLCCWVEN PNSEAFKKHL PRVLDYLWLA EDGMKMQGYN GTQLWDTAFA
     VQALVSTQLL DECGSMLKKA HHYIERSQVQ EDCAGDLRRW FRHISKGAWP FSTRDHGWPI
     SDCSSEGLKA SLELSSLSGD LVGDPIPAQR LYDCVNVILS YQNPNGGIAT YESTRSYAWL
     ELLNPAETFG DIVIDYPCVE CTSACVQALA AFKRKYPEHR SKEIATTIRQ GSMYIKNVQR
     SDGSWYGSWG VCFTYGTWFG IMGLLASGET YSTSASLRNA CSFLLSKQLP SGGWGESYLS
     SQDKTYTHLE GDEAHLVNTS WAMLALIASG QVERDPTPLH KAXVLLVNAQ FENGDYPQQE
     IIGVFNRNCM ISYSAYRNIF PIWALGAYQC KVLGH