ID   LCD1_KLULA              Reviewed;         664 AA.
AC   Q6CUV9;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=DNA damage checkpoint protein LCD1;
GN   Name=LCD1; OrderedLocusNames=KLLA0C01892g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Forms a complex with the serine/threonine kinase MEC1 which
CC       activates checkpoint signaling upon genotoxic stresses. The MEC1-LCD1
CC       complex is recruited to DNA lesions in order to initiates the DNA
CC       repair by homologous recombination. Required for cell growth and
CC       meiotic recombination (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Forms a complex with MEC1. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
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DR   EMBL; CR382123; CAH01131.1; -; Genomic_DNA.
DR   RefSeq; XP_452280.1; XM_452280.1.
DR   PDB; 5OMB; X-ray; 1.94 A; C/D=1-109.
DR   PDB; 5OMC; X-ray; 2.38 A; C/D=1-109.
DR   PDBsum; 5OMB; -.
DR   PDBsum; 5OMC; -.
DR   AlphaFoldDB; Q6CUV9; -.
DR   SMR; Q6CUV9; -.
DR   STRING; 28985.XP_452280.1; -.
DR   EnsemblFungi; CAH01131; CAH01131; KLLA0_C01892g.
DR   GeneID; 2892543; -.
DR   KEGG; kla:KLLA0_C01892g; -.
DR   eggNOG; ENOG502QQI0; Eukaryota.
DR   HOGENOM; CLU_402302_0_0_1; -.
DR   InParanoid; Q6CUV9; -.
DR   OMA; IFQYELI; -.
DR   Proteomes; UP000000598; Chromosome C.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0006325; P:chromatin organization; IEA:UniProtKB-KW.
DR   GO; GO:0000077; P:DNA damage checkpoint signaling; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   InterPro; IPR018622; DNA_damage_chkpnt_Lcd1.
DR   Pfam; PF09798; LCD1; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Chromatin regulator; Coiled coil; Cytoplasm; DNA damage;
KW   DNA repair; Nucleus; Phosphoprotein; Reference proteome.
FT   CHAIN           1..664
FT                   /note="DNA damage checkpoint protein LCD1"
FT                   /id="PRO_0000227714"
FT   REGION          60..81
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          109..132
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          38..107
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        116..132
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   HELIX           12..15
FT                   /evidence="ECO:0007829|PDB:5OMB"
FT   TURN            16..19
FT                   /evidence="ECO:0007829|PDB:5OMB"
FT   HELIX           36..103
FT                   /evidence="ECO:0007829|PDB:5OMB"
SQ   SEQUENCE   664 AA;  76860 MW;  AFC7E246BF563E3B CRC64;
     MADLWDDNDD DDDILELVNR PPMSQMAVPI KPPESQAEQL MKAKGEVGVL RQKLSMLEKT
     LREHDDNQKK LESSLKSSHE EEVTKLKIEL ERLEDERKFM LLEQKHLFTP RNSSKSETTH
     ETETAEPSPI ENKRRRMEPV KEYVTLTQNF KVDDGSLFYD HLLNFKLLGS EHTVLEMLDH
     ICTYKTDLIL SPEHKIIKAR LPLGPPIRSL IFKWKSIYTL DQLVDKTLEI LAIAIKSVSV
     LPENKLAIPF LISLMHCTIN FRHSATSVSS LKDVFQFITD FIISDPKFLK QPLHESPLDL
     DVSPDVFQYS MLDQLCMTYS FDVLETCIVI LLNCNTRAQE IVLNDATIAE NLIKCCNFTL
     SISYKPIMPI IVNVTEILLG IVELDGAENV WKGKWAVLFS KLIQNWERSL TFDSVSLNFA
     GLNRCCGDNS NHSMIDNIIS LEEVRYLPMI IENEFEPLTF TALDNLEYWS IQIQINITLI
     MHKLLHRYRS TLCSLEFLQK TFSLFSSHQE LLLTVFLDRD HISDIKRQEL LSVLLKLIYF
     CWVSISDEYK RALRLDELTI CLWRIVYGWP ESTDELSTEW AALINPLKAL ALEEEKKYFD
     DAYDEDNLPA FMHSEELDIK RESAVSKFNN NSSWPLRDMA KYILESITTM DEADSLYVAM
     VSET