LCFC1_MOUSE
ID LCFC1_MOUSE Reviewed; 108 AA.
AC Q9D9P8;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Sperm-egg fusion protein LLCFC1 {ECO:0000305};
DE AltName: Full=LLLL and CFNLAS motif-containing protein 1 {ECO:0000250|UniProtKB:Q96L11};
DE AltName: Full=MSSP-binding protein CTM-1 {ECO:0000250|UniProtKB:Q96L11};
DE AltName: Full=Sperm-oocyte fusion required protein 1 {ECO:0000303|PubMed:32393636};
DE Flags: Precursor;
GN Name=Llcfc1 {ECO:0000312|MGI:MGI:1923856};
GN Synonyms=Sof1 {ECO:0000303|PubMed:32393636};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=32393636; DOI=10.1073/pnas.1922650117;
RA Noda T., Lu Y., Fujihara Y., Oura S., Koyano T., Kobayashi S., Matzuk M.M.,
RA Ikawa M.;
RT "Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte
RT fusion in mice.";
RL Proc. Natl. Acad. Sci. U.S.A. 117:11493-11502(2020).
CC -!- FUNCTION: Sperm protein required for fusion of sperm with the egg
CC membrane during fertilization. {ECO:0000269|PubMed:32393636}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Detected in testicular germ cells and spermatozoa
CC (at protein level). Abundantly expressed in testis.
CC {ECO:0000269|PubMed:32393636}.
CC -!- DEVELOPMENTAL STAGE: Detected in the testis after postnatal day 28.
CC {ECO:0000269|PubMed:32393636}.
CC -!- DISRUPTION PHENOTYPE: Male infertility (PubMed:32393636). Sperm are
CC morphologically normal, exhibit normal motility and can penetrate the
CC zona pellucida but are unable to fuse with the egg membrane
CC (PubMed:32393636). No effect on amount or localization of sperm-egg
CC fusion protein IZUMO1 (PubMed:32393636). {ECO:0000269|PubMed:32393636}.
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DR EMBL; AK006606; BAB24671.1; -; mRNA.
DR EMBL; AC156400; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466533; EDL13502.1; -; Genomic_DNA.
DR EMBL; BC048592; AAH48592.1; -; mRNA.
DR CCDS; CCDS20054.1; -.
DR RefSeq; NP_083947.1; NM_029671.2.
DR AlphaFoldDB; Q9D9P8; -.
DR BioGRID; 218200; 1.
DR STRING; 10090.ENSMUSP00000031900; -.
DR PaxDb; Q9D9P8; -.
DR PRIDE; Q9D9P8; -.
DR Antibodypedia; 50550; 58 antibodies from 8 providers.
DR Ensembl; ENSMUST00000031900; ENSMUSP00000031900; ENSMUSG00000029867.
DR GeneID; 76606; -.
DR KEGG; mmu:76606; -.
DR UCSC; uc009bqe.1; mouse.
DR CTD; 135927; -.
DR MGI; MGI:1923856; Llcfc1.
DR VEuPathDB; HostDB:ENSMUSG00000029867; -.
DR eggNOG; ENOG502SF8S; Eukaryota.
DR GeneTree; ENSGT00390000018785; -.
DR HOGENOM; CLU_1969769_0_0_1; -.
DR InParanoid; Q9D9P8; -.
DR OMA; LNIHLCF; -.
DR OrthoDB; 1571373at2759; -.
DR PhylomeDB; Q9D9P8; -.
DR TreeFam; TF338373; -.
DR BioGRID-ORCS; 76606; 0 hits in 72 CRISPR screens.
DR PRO; PR:Q9D9P8; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q9D9P8; protein.
DR Bgee; ENSMUSG00000029867; Expressed in spermatid and 11 other tissues.
DR Genevisible; Q9D9P8; MM.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; IMP:UniProtKB.
DR InterPro; IPR031684; LLCFC1.
DR PANTHER; PTHR37348; PTHR37348; 1.
DR Pfam; PF15838; LLCFC1; 1.
PE 1: Evidence at protein level;
KW Fertilization; Reference proteome; Secreted; Signal.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..108
FT /note="Sperm-egg fusion protein LLCFC1"
FT /id="PRO_0000352881"
FT REGION 39..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 39..53
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 108 AA; 12138 MW; 154E27F37C5B6101 CRC64;
MTSLGSQLHR ATFLTALLLL LLLQVKGVKT LIVSASLDGD KSQKDKVSSE DQGEEEYEEH
FEASSEGEQW QEIDMVQQED TISQAITLQD HLLDLAFCFN LASIMFFL
