LCK_AEDAE
ID LCK_AEDAE Reviewed; 228 AA.
AC O02036; Q16TP4;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=Leucokinins;
DE Contains:
DE RecName: Full=Leucokinin-1;
DE Contains:
DE RecName: Full=Leucokinin-3;
DE Contains:
DE RecName: Full=Leucokinin-2;
DE Flags: Precursor;
GN ORFNames=AAEL010172;
OS Aedes aegypti (Yellowfever mosquito) (Culex aegypti).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Culicinae; Aedini; Aedes; Stegomyia.
OX NCBI_TaxID=7159;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC TISSUE=Abdominal ganglion;
RX PubMed=9099680; DOI=10.1074/jbc.272.16.10402;
RA Veenstra J.A., Patillo J.M., Petzel D.H.;
RT "A single cDNA encodes all three Aedes leucokinins, which stimulate both
RT fluid secretion by the Malpighian tubules and hindgut contractions.";
RL J. Biol. Chem. 272:10402-10407(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LVPib12;
RX PubMed=17510324; DOI=10.1126/science.1138878;
RA Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J.,
RA Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F.,
RA Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.,
RA Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L.,
RA Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J.,
RA Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E.,
RA El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I.,
RA Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H.,
RA Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S.,
RA Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P.,
RA Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J.,
RA Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W.,
RA Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O.,
RA Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R.,
RA Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B.,
RA Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T.,
RA Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.;
RT "Genome sequence of Aedes aegypti, a major arbovirus vector.";
RL Science 316:1718-1723(2007).
RN [3]
RP PARTIAL PROTEIN SEQUENCE (LEUCOKININS), AND AMIDATION AT GLY-180; GLY-193
RP AND GLY-217.
RX PubMed=8048942; DOI=10.1006/bbrc.1994.1989;
RA Veenstra J.A.;
RT "Isolation and identification of three leucokinins from the mosquito Aedes
RT aegypti.";
RL Biochem. Biophys. Res. Commun. 202:715-719(1994).
CC -!- FUNCTION: Stimulates both fluid secretion by the Malpighian tubules and
CC hindgut contractions. Depolarize the transepithelial voltage of the
CC Malpighian tubules in concentrations of less than 10(-9) M and increase
CC the frequency of hindgut contractions at concentrations above 10(-8) M.
CC {ECO:0000269|PubMed:9099680}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:9099680}.
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DR EMBL; U66832; AAC47656.1; -; mRNA.
DR EMBL; CH477643; EAT37886.1; -; Genomic_DNA.
DR RefSeq; XP_001654286.1; XM_001654236.1.
DR AlphaFoldDB; O02036; -.
DR STRING; 7159.AAEL010172-PA; -.
DR GeneID; 5572957; -.
DR KEGG; aag:5572957; -.
DR VEuPathDB; VectorBase:AAEL010172; -.
DR eggNOG; ENOG502TMCQ; Eukaryota.
DR HOGENOM; CLU_1215651_0_0_1; -.
DR InParanoid; O02036; -.
DR OMA; GCEWTSR; -.
DR OrthoDB; 1056742at2759; -.
DR PhylomeDB; O02036; -.
DR Proteomes; UP000008820; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW Neuropeptide; Reference proteome; Secreted; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT PROPEP 19..164
FT /id="PRO_0000000940"
FT PEPTIDE 167..180
FT /note="Leucokinin-1"
FT /id="PRO_0000000941"
FT PEPTIDE 184..193
FT /note="Leucokinin-3"
FT /id="PRO_0000000942"
FT PROPEP 197..209
FT /id="PRO_0000000943"
FT PEPTIDE 211..217
FT /note="Leucokinin-2"
FT /id="PRO_0000000944"
FT PROPEP 221..228
FT /id="PRO_0000000945"
FT REGION 80..118
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 88..104
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 180
FT /note="Glycine amide"
FT /evidence="ECO:0000269|PubMed:8048942"
FT MOD_RES 193
FT /note="Glycine amide"
FT /evidence="ECO:0000269|PubMed:8048942"
FT MOD_RES 217
FT /note="Glycine amide"
FT /evidence="ECO:0000269|PubMed:8048942"
SQ SEQUENCE 228 AA; 25934 MW; B97378F01D54D504 CRC64;
MAMLLQVALP LLAAVSWGWE LNENDDSLAK IIEGCEWTSR QNVISEILLD RYRKYAMYNF
FLLDDVCAVH EWNKNLKEPE FSENNEAEDK SPTSAQNTQE HIPGNNFPPP AASNPPVNSS
CAKSAKDFFI CLSNQLGDPT LNAMLLDNLE VACDPRFSPV SAIQKRNSKY VSKQKFYSWG
GKRNNPNVFY PWGGKRNTGR VHRQPKVVIR NPFHAWGGKR NQKDDNVF
