LCK_DROME
ID LCK_DROME Reviewed; 160 AA.
AC P81829; Q4V3G8; Q9U4A5; Q9VUF4;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 15-AUG-2003, sequence version 3.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Leucokinin;
DE Short=DLK;
DE Flags: Precursor;
GN Name=Lk; Synonyms=pp; ORFNames=CG13480;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 117-160, PROTEIN SEQUENCE OF 133-147, AND
RP AMIDATION.
RC STRAIN=Oregon-R; TISSUE=Neurosecretory cell;
RX PubMed=10574744; DOI=10.1242/jeb.202.24.3667;
RA Terhzaz S., O'Connell F.C., Pollock V.P., Kean L., Davies S.A.,
RA Veenstra J.A., Dow J.A.T.;
RT "Isolation and characterization of a leucokinin-like peptide of Drosophila
RT melanogaster.";
RL J. Exp. Biol. 202:3667-3676(1999).
RN [5]
RP PROTEIN SEQUENCE OF 133-147, AND AMIDATION AT GLY-147.
RC TISSUE=Larva;
RX PubMed=12171930; DOI=10.1074/jbc.m206257200;
RA Baggerman G., Cerstiaens A., De Loof A., Schoofs L.;
RT "Peptidomics of the larval Drosophila melanogaster central nervous
RT system.";
RL J. Biol. Chem. 277:40368-40374(2002).
CC -!- FUNCTION: Acts through intracellular calcium in Malpighian tubule
CC stellate cells to raise chloride conductance.
CC -!- SUBCELLULAR LOCATION: Secreted.
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DR EMBL; AE014296; AAF49731.2; -; Genomic_DNA.
DR EMBL; BT023388; AAY55804.1; -; mRNA.
DR EMBL; AF192342; AAF20066.1; -; mRNA.
DR RefSeq; NP_524893.2; NM_080154.5.
DR AlphaFoldDB; P81829; -.
DR STRING; 7227.FBpp0075465; -.
DR PaxDb; P81829; -.
DR PRIDE; P81829; -.
DR DNASU; 47746; -.
DR EnsemblMetazoa; FBtr0075722; FBpp0075465; FBgn0028418.
DR GeneID; 47746; -.
DR KEGG; dme:Dmel_CG13480; -.
DR UCSC; CG13480-RA; d. melanogaster.
DR CTD; 47746; -.
DR FlyBase; FBgn0028418; Lk.
DR VEuPathDB; VectorBase:FBgn0028418; -.
DR eggNOG; ENOG502TAY3; Eukaryota.
DR HOGENOM; CLU_1679752_0_0_1; -.
DR InParanoid; P81829; -.
DR OMA; RGEIWAF; -.
DR OrthoDB; 1408199at2759; -.
DR PhylomeDB; P81829; -.
DR BioGRID-ORCS; 47746; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 47746; -.
DR PRO; PR:P81829; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0028418; Expressed in mouthpart and 11 other tissues.
DR ExpressionAtlas; P81829; baseline and differential.
DR Genevisible; P81829; DM.
DR GO; GO:0005615; C:extracellular space; ISM:FlyBase.
DR GO; GO:0008613; F:diuretic hormone activity; IDA:FlyBase.
DR GO; GO:0005179; F:hormone activity; NAS:FlyBase.
DR GO; GO:0005184; F:neuropeptide hormone activity; IMP:FlyBase.
DR GO; GO:0048018; F:receptor ligand activity; IDA:FlyBase.
DR GO; GO:0007589; P:body fluid secretion; IDA:FlyBase.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IDA:FlyBase.
DR GO; GO:2000252; P:negative regulation of feeding behavior; IMP:FlyBase.
DR GO; GO:0007218; P:neuropeptide signaling pathway; NAS:FlyBase.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IDA:FlyBase.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW Neuropeptide; Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..130
FT /id="PRO_0000021580"
FT PEPTIDE 133..147
FT /note="Leucokinin"
FT /id="PRO_0000021581"
FT PROPEP 151..160
FT /id="PRO_0000021582"
FT MOD_RES 147
FT /note="Glycine amide"
FT /evidence="ECO:0000269|PubMed:12171930"
SQ SEQUENCE 160 AA; 18277 MW; 8E9C41B2086407EA CRC64;
MAKIVLCMVL LAFGRQVYGA SLVPAPISEQ DPELATCELQ LSKYRRFILQ AILSFEDVCD
AYSSRPGGQD SDSEGWPFRH YAPPPTSQRG EIWAFFRLLM AQFGDKEFSP IIRDAVIERC
RIKSQLQRDE KRNSVVLGKK QRFHSWGGKR SPEPPILPDY
