ID   LCL3_TALMQ              Reviewed;         344 AA.
AC   B6QNP4;
DT   31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   16-DEC-2008, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Probable endonuclease lcl3;
DE            EC=3.1.-.-;
GN   Name=lcl3; ORFNames=PMAA_053390;
OS   Talaromyces marneffei (strain ATCC 18224 / CBS 334.59 / QM 7333)
OS   (Penicillium marneffei).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Trichocomaceae; Talaromyces;
OC   Talaromyces sect. Talaromyces.
OX   NCBI_TaxID=441960;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 18224 / CBS 334.59 / QM 7333;
RX   PubMed=25676766; DOI=10.1128/genomea.01559-14;
RA   Nierman W.C., Fedorova-Abrams N.D., Andrianopoulos A.;
RT   "Genome sequence of the AIDS-associated pathogen Penicillium marneffei
RT   (ATCC18224) and its near taxonomic relative Talaromyces stipitatus
RT   (ATCC10500).";
RL   Genome Announc. 3:E0155914-E0155914(2015).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion. Membrane {ECO:0000250}; Single-
CC       pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the LCL3 family. {ECO:0000305}.
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DR   EMBL; DS995903; EEA21532.1; -; Genomic_DNA.
DR   RefSeq; XP_002150141.1; XM_002150105.1.
DR   AlphaFoldDB; B6QNP4; -.
DR   SMR; B6QNP4; -.
DR   STRING; 441960.B6QNP4; -.
DR   EnsemblFungi; EEA21532; EEA21532; PMAA_053390.
DR   GeneID; 7027666; -.
DR   KEGG; tmf:PMAA_053390; -.
DR   VEuPathDB; FungiDB:PMAA_053390; -.
DR   HOGENOM; CLU_046484_0_1_1; -.
DR   OrthoDB; 1333771at2759; -.
DR   PhylomeDB; B6QNP4; -.
DR   Proteomes; UP000001294; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.90; -; 1.
DR   InterPro; IPR035437; SNase_OB-fold_sf.
DR   InterPro; IPR016071; Staphylococal_nuclease_OB-fold.
DR   Pfam; PF00565; SNase; 1.
DR   SMART; SM00318; SNc; 1.
DR   SUPFAM; SSF50199; SSF50199; 1.
DR   PROSITE; PS50830; TNASE_3; 1.
PE   3: Inferred from homology;
KW   Calcium; Endonuclease; Hydrolase; Membrane; Metal-binding; Mitochondrion;
KW   Nuclease; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..344
FT                   /note="Probable endonuclease lcl3"
FT                   /id="PRO_0000408674"
FT   TRANSMEM        77..93
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          115..282
FT                   /note="TNase-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00272"
FT   REGION          1..55
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          291..327
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        15..31
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        32..55
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        303..325
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        166
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00272"
FT   ACT_SITE        174
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00272"
FT   ACT_SITE        214
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00272"
FT   BINDING         171
FT                   /ligand="Ca(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29108"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00272"
SQ   SEQUENCE   344 AA;  39258 MW;  B60B6C6FD10EEB64 CRC64;
     MGWWSLGSSG SKADPEKSKA NDSKSSNRRQ QEEGEQSFIP PPLTSRTSSS SSSKSTTDWN
     SSLNAFDWSQ FKQPRNLIPT ALLTGGILFV VYVQRRYLRR FPEATDISSS YFRSRSLLGR
     VTSVGDGDNF RIFHTPGGRL VGWGWLPWMK VPTARKELKD KTVHIRLAGV DAPELAHFGR
     PAQPYAYEAH MWLTSYLMNR RVRAYVHRPD QYKRVIATVY VRRWLDFPPL RRRDVSYEML
     RRGLATVYEA KSGVEFGGTE NERKYREAEM LAKNRRQGLW KDFGKRGGVN FESPREYKTR
     MQSLDMSAES SSSSSSSSNT EKNPGLVGSL LRKVWPFGSK KDGT