LCLT1_DANRE
ID LCLT1_DANRE Reviewed; 388 AA.
AC Q6NYV8;
DT 26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Lysocardiolipin acyltransferase 1;
DE EC=2.3.1.- {ECO:0000250|UniProtKB:Q3UN02};
DE AltName: Full=1-acylglycerol-3-phosphate O-acyltransferase 8 {ECO:0000250|UniProtKB:Q6UWP7};
DE Short=1-AGP acyltransferase 8;
DE Short=1-AGPAT 8;
DE EC=2.3.1.51 {ECO:0000250|UniProtKB:Q6UWP7};
DE AltName: Full=Acyl-CoA:lysocardiolipin acyltransferase 1 {ECO:0000250|UniProtKB:Q3UN02};
GN Name=lclat1; Synonyms=agpat8 {ECO:0000250|UniProtKB:Q6UWP7}, lycat;
GN ORFNames=zgc:77380;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION, AND MUTAGENESIS OF GLU-165 AND GLY-166.
RX PubMed=18388326; DOI=10.1161/circresaha.107.163907;
RA Xiong J.-W., Yu Q., Zhang J., Mably J.D.;
RT "An acyltransferase controls the generation of hematopoietic and
RT endothelial lineages in zebrafish.";
RL Circ. Res. 102:1057-1064(2008).
CC -!- FUNCTION: Exhibits acyl-CoA:lysocardiolipin acyltransferase (ALCAT)
CC activity; catalyzes the reacylation of lyso-cardiolipin to cardiolipin
CC (CL), a key step in CL remodeling (By similarity). Recognizes both
CC monolysocardiolipin and dilysocardiolipin as substrates with a
CC preference for linoleoyl-CoA and oleoyl-CoA as acyl donors (By
CC similarity). Also exhibits 1-acyl-sn-glycerol-3-phosphate
CC acyltransferase activity (AGPAT) activity; converts 1-acyl-sn-glycerol-
CC 3- phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-
CC glycerol-3- phosphate (phosphatidic acid or PA) by incorporating an
CC acyl moiety at the sn-2 position of the glycerol backbone (By
CC similarity). Possesses both lysophosphatidylinositol acyltransferase
CC (LPIAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities
CC (By similarity). Required for establishment of the hematopoietic and
CC endothelial lineages (PubMed:18388326). {ECO:0000250|UniProtKB:Q3UN02,
CC ECO:0000250|UniProtKB:Q6UWP7, ECO:0000269|PubMed:18388326}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-
CC sn-glycero-3-phosphate + CoA; Xref=Rhea:RHEA:19709,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57970, ChEBI:CHEBI:58342,
CC ChEBI:CHEBI:58608; EC=2.3.1.51;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19710;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1-acyl-sn-glycero-3-phospho-(1D-myo-inositol) + an acyl-CoA
CC = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + CoA;
CC Xref=Rhea:RHEA:33195, ChEBI:CHEBI:57287, ChEBI:CHEBI:57880,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64771;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33196;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-acyl-sn-glycero-3-phospho-(1'-sn-glycerol) + an acyl-CoA =
CC 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + CoA;
CC Xref=Rhea:RHEA:33203, ChEBI:CHEBI:57287, ChEBI:CHEBI:58342,
CC ChEBI:CHEBI:64716, ChEBI:CHEBI:64840;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33204;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-hexadecanoyl-sn-glycero-3-phosphate
CC = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA;
CC Xref=Rhea:RHEA:33187, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC ChEBI:CHEBI:57518, ChEBI:CHEBI:64839;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33188;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-
CC phosphate = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA;
CC Xref=Rhea:RHEA:37131, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC ChEBI:CHEBI:74544, ChEBI:CHEBI:74546;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37132;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-(9Z,12Z)-octadecadienoyl-sn-glycero-
CC 3-phosphate = 1-(9Z,12Z)-octadecadienoyl-2-(9Z)-octadecenoyl-sn-
CC glycero-3-phosphate + CoA; Xref=Rhea:RHEA:37135, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57387, ChEBI:CHEBI:74547, ChEBI:CHEBI:74548;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37136;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-(9Z,12Z,15Z)-octadecatrienoyl-sn-
CC glycero-3-phosphate = 1-(9Z,12Z,15Z)-octadecatrienoyl-2-(9Z)-
CC octadecenoyl-sn-glycero-3-phosphate + CoA; Xref=Rhea:RHEA:37139,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:74549,
CC ChEBI:CHEBI:74550; Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37140;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + hexadecanoyl-CoA
CC = 1-(9Z)-octadecenoyl-2-hexadecanoyl-sn-glycero-3-phosphate + CoA;
CC Xref=Rhea:RHEA:37143, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379,
CC ChEBI:CHEBI:74544, ChEBI:CHEBI:74551;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37144;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + octadecanoyl-CoA
CC = 1-(9Z-octadecenoyl)-2-octadecanoyl-sn-glycero-3-phosphate + CoA;
CC Xref=Rhea:RHEA:37147, ChEBI:CHEBI:57287, ChEBI:CHEBI:57394,
CC ChEBI:CHEBI:74544, ChEBI:CHEBI:74552;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37148;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-acyl-sn-glycero-3-phospho-(1'-sn-
CC glycerol) = 1-acyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-
CC glycerol) + CoA; Xref=Rhea:RHEA:37619, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57387, ChEBI:CHEBI:64840, ChEBI:CHEBI:75173;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37620;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + a 1-acyl-sn-glycero-3-phospho-(1D-myo-
CC inositol) = a 1-acyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1D-
CC myo-inositol) + CoA; Xref=Rhea:RHEA:37623, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57387, ChEBI:CHEBI:64771, ChEBI:CHEBI:75116;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37624;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-hexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol) +
CC hexadecanoyl-CoA = 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-
CC inositol) + CoA; Xref=Rhea:RHEA:35871, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57379, ChEBI:CHEBI:72833, ChEBI:CHEBI:72835;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35872;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-hexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol) +
CC octadecanoyl-CoA = 1-hexadecanoyl-2-octadecanoyl-sn-glycero-3-
CC phospho-(1D-myo-inositol) + CoA; Xref=Rhea:RHEA:35875,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57394, ChEBI:CHEBI:72833,
CC ChEBI:CHEBI:72836; Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35876;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-hexadecanoyl-sn-glycero-3-phospho-
CC (1D-myo-inositol) = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-
CC phospho-(1D-myo-inositol) + CoA; Xref=Rhea:RHEA:35879,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:72833,
CC ChEBI:CHEBI:72837; Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35880;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z,12Z)-octadecadienoyl-CoA + 1-hexadecanoyl-sn-glycero-3-
CC phospho-(1D-myo-inositol) = 1-hexadecanoyl-2-(9Z,12Z-
CC octadecadienoyl)-sn-glycero-3-phospho-(1D-myo-inositol) + CoA;
CC Xref=Rhea:RHEA:35883, ChEBI:CHEBI:57287, ChEBI:CHEBI:57383,
CC ChEBI:CHEBI:72833, ChEBI:CHEBI:72838;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35884;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 1-hexadecanoyl-sn-
CC glycero-3-phospho-(1D-myo-inositol) = 1-hexadecanoyl-2-
CC (5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phospho-D-myo-inositol
CC + CoA; Xref=Rhea:RHEA:35867, ChEBI:CHEBI:57287, ChEBI:CHEBI:57368,
CC ChEBI:CHEBI:72833, ChEBI:CHEBI:72834;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35868;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-hexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) +
CC hexadecanoyl-CoA = 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1'-sn-
CC glycerol) + CoA; Xref=Rhea:RHEA:35851, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57379, ChEBI:CHEBI:72829, ChEBI:CHEBI:75158;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35852;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-hexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) +
CC octadecanoyl-CoA = 1-hexadecanoyl-2-octadecanoyl-sn-glycero-3-
CC phospho-(1'-sn-glycerol) + CoA; Xref=Rhea:RHEA:35887,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57394, ChEBI:CHEBI:72839,
CC ChEBI:CHEBI:75158; Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35888;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-hexadecanoyl-sn-glycero-3-phospho-
CC (1'-sn-glycerol) = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-
CC phospho-(1'-sn-glycerol) + CoA; Xref=Rhea:RHEA:35891,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:72841,
CC ChEBI:CHEBI:75158; Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35892;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z,12Z)-octadecadienoyl-CoA + 1-hexadecanoyl-sn-glycero-3-
CC phospho-(1'-sn-glycerol) = 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-
CC sn-glycero-3-phospho-(1'-sn-glycerol) + CoA; Xref=Rhea:RHEA:35895,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57383, ChEBI:CHEBI:72840,
CC ChEBI:CHEBI:75158; Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35896;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-tetradecanoyl-sn-glycero-3-phospho-
CC (1'-sn-glycerol) = 1-tetradecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-
CC phospho-(1'-sn-glycerol) + CoA; Xref=Rhea:RHEA:37643,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:72826,
CC ChEBI:CHEBI:75161; Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37644;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-octadecanoyl-sn-glycero-3-phospho-
CC (1'-sn-glycerol) = 1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-
CC phospho-(1'-sn-glycerol) + CoA; Xref=Rhea:RHEA:37647,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:72827,
CC ChEBI:CHEBI:72845; Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37648;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-
CC phospho-(1'-sn-glycerol) = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-
CC phospho-(1'-sn-glycerol) + CoA; Xref=Rhea:RHEA:37651,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:72828,
CC ChEBI:CHEBI:75163; Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37652;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-hexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol) +
CC dodecanoyl-CoA = 1-hexadecanoyl-2-dodecanoyl-sn-glycero-3-phospho-
CC (1D-myo-inositol) + CoA; Xref=Rhea:RHEA:37639, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57375, ChEBI:CHEBI:72833, ChEBI:CHEBI:75160;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37640;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1',3'-bis-[1-acyl-sn-glycero-3-
CC phospho]-glycerol = 1'-[1-acyl-2-(9Z)-octadecenoyl-sn-glycero-3-
CC phospho],3'-[1-acyl,2-hydroxy-sn-glycero-3-phospho]-glycerol + CoA;
CC Xref=Rhea:RHEA:37615, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC ChEBI:CHEBI:75137, ChEBI:CHEBI:75139;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37616;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1'-[1,2-diacyl-sn-glycero-3-
CC phospho],3'-[1-acyl-sn-glycero-3-phospho]-glycerol = 1'-[1,2-diacyl-
CC sn-glycero-3-phospho],3'-[1-acyl,2-(9Z)-octadecenoyl-sn-glycero-3-
CC phospho]-glycerol + CoA; Xref=Rhea:RHEA:37611, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57387, ChEBI:CHEBI:64743, ChEBI:CHEBI:75140;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37612;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z,12Z)-octadecadienoyl-CoA + 1'-[1,2-diacyl-sn-glycero-3-
CC phospho],3'-[1-acyl-sn-glycero-3-phospho]-glycerol = 1'-[1,2-diacyl-
CC sn-glycero-3-phospho],3'-[1-acyl,2-(9Z,12Z)-octadecadienoyl-sn-
CC glycero-3-phospho]-glycerol + CoA; Xref=Rhea:RHEA:37675,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57383, ChEBI:CHEBI:64743,
CC ChEBI:CHEBI:75205; Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37676;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1'-[1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl-sn-glycero-3-
CC phospho]-glycerol + dodecanoyl-CoA = 1'-[1,2-diacyl-sn-glycero-3-
CC phospho],3'-[1-acyl,2-dodecanoyl-sn-glycero-3-phospho]-glycerol +
CC CoA; Xref=Rhea:RHEA:37679, ChEBI:CHEBI:57287, ChEBI:CHEBI:57375,
CC ChEBI:CHEBI:64743, ChEBI:CHEBI:75203;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37680;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1',3'-bis-[1-acyl-sn-glycero-3-phospho]-glycerol + dodecanoyl-
CC CoA = 1'-[1-acyl-2-dodecanoyl-sn-glycero-3-phospho],3'-[1-acyl,2-
CC hydroxy-sn-glycero-3-phospho]-glycerol + CoA; Xref=Rhea:RHEA:37683,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57375, ChEBI:CHEBI:75137,
CC ChEBI:CHEBI:75201; Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37684;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + a 1-acyl-sn-glycero-3-phosphate = a 1-
CC acyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA;
CC Xref=Rhea:RHEA:37427, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC ChEBI:CHEBI:57970, ChEBI:CHEBI:74917;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37428;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z,12Z)-octadecadienoyl-CoA + 1',3'-bis-[1-acyl-sn-glycero-3-
CC phospho]-glycerol = 1'-[1-acyl-2-(9Z,12Z)-octadecadienoyl-sn-glycero-
CC 3-phospho],3'-[1-acyl,2-hydroxy-sn-glycero-3-phospho]-glycerol + CoA;
CC Xref=Rhea:RHEA:37687, ChEBI:CHEBI:57287, ChEBI:CHEBI:57383,
CC ChEBI:CHEBI:75137, ChEBI:CHEBI:75209;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37688;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1',3'-bis-[1-acyl-sn-glycero-3-phospho]-glycerol +
CC hexadecanoyl-CoA = 1'-[1-acyl-2-hexadecanoyl-sn-glycero-3-
CC phospho],3'-[1-acyl,2-hydroxy-sn-glycero-3-phospho]-glycerol + CoA;
CC Xref=Rhea:RHEA:37691, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379,
CC ChEBI:CHEBI:75137, ChEBI:CHEBI:75207;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37692;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1',3'-bis-[1-acyl-sn-glycero-3-phospho]-glycerol +
CC octadecanoyl-CoA = 1'-[1-acyl-2-octadecanoyl-sn-glycero-3-
CC phospho],3'-[1-acyl,2-hydroxy-sn-glycero-3-phospho]-glycerol + CoA;
CC Xref=Rhea:RHEA:37695, ChEBI:CHEBI:57287, ChEBI:CHEBI:57394,
CC ChEBI:CHEBI:75137, ChEBI:CHEBI:75208;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37696;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1'-[1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl-sn-glycero-3-
CC phospho]-glycerol + octanoyl-CoA = 1'-[1,2-diacyl-sn-glycero-3-
CC phospho],3'-[1-acyl,2-octanoyl-sn-glycero-3-phospho]-glycerol + CoA;
CC Xref=Rhea:RHEA:38623, ChEBI:CHEBI:57287, ChEBI:CHEBI:57386,
CC ChEBI:CHEBI:64743, ChEBI:CHEBI:75990;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38624;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1',3'-bis-[1-acyl-sn-glycero-3-phospho]-glycerol + octanoyl-
CC CoA = 1'-[1-acyl-2-octanoyl-sn-glycero-3-phospho],3'-[1-acyl,2-
CC hydroxy-sn-glycero-3-phospho]-glycerol + CoA; Xref=Rhea:RHEA:38627,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57386, ChEBI:CHEBI:75137,
CC ChEBI:CHEBI:75993; Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38628;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1'-[1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl-sn-glycero-3-
CC phospho]-glycerol + hexadecanoyl-CoA = 1'-[1,2-diacyl-sn-glycero-3-
CC phospho],3'-[1-acyl,2-hexadecanoyl-sn-glycero-3-phospho]-glycerol +
CC CoA; Xref=Rhea:RHEA:38631, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379,
CC ChEBI:CHEBI:64743, ChEBI:CHEBI:75994;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38632;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 1'-[1,2-diacyl-sn-
CC glycero-3-phospho],3'-[1-acyl-sn-glycero-3-phospho]-glycerol = 1'-
CC [1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl,2-(5Z,8Z,11Z,14Z)-
CC eicosatetraenoyl-sn-glycero-3-phospho]-glycerol + CoA;
CC Xref=Rhea:RHEA:38635, ChEBI:CHEBI:57287, ChEBI:CHEBI:57368,
CC ChEBI:CHEBI:64743, ChEBI:CHEBI:75995;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38636;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 1',3'-bis-[1-acyl-sn-
CC glycero-3-phospho]-glycerol = 1'-[1-acyl-2-(5Z,8Z,11Z,14Z)-
CC eicosatetraenoyl-sn-glycero-3-phospho],3'-[1-acyl,2-hydroxy-sn-
CC glycero-3-phospho]-glycerol + CoA; Xref=Rhea:RHEA:38639,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57368, ChEBI:CHEBI:75137,
CC ChEBI:CHEBI:75996; Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38640;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1-acyl-sn-glycero-3-phospho-(1D-myo-inositol) +
CC octadecanoyl-CoA = a 1-acyl-2-octadecanoyl-sn-glycero-3-phospho-(1D-
CC myo-inositol) + CoA; Xref=Rhea:RHEA:43960, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57394, ChEBI:CHEBI:64771, ChEBI:CHEBI:83939;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43961;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2-acyl-sn-glycero-3-phospho-D-myo-inositol + octadecanoyl-
CC CoA = 1-octadecanoyl-2-acyl-sn-glycero-3-phospho-(1D-myo-inositol) +
CC CoA; Xref=Rhea:RHEA:43964, ChEBI:CHEBI:57287, ChEBI:CHEBI:57394,
CC ChEBI:CHEBI:64872, ChEBI:CHEBI:83940;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43965;
CC Evidence={ECO:0000250|UniProtKB:Q3UN02};
CC -!- PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-
CC diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q6UWP7}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- DOMAIN: The HXXXXD motif is essential for acyltransferase activity and
CC may constitute the binding site for the phosphate moiety of the
CC glycerol-3-phosphate. {ECO:0000250|UniProtKB:Q9D517}.
CC -!- SIMILARITY: Belongs to the 1-acyl-sn-glycerol-3-phosphate
CC acyltransferase family. {ECO:0000305}.
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DR EMBL; BC066444; AAH66444.1; -; mRNA.
DR RefSeq; NP_998435.1; NM_213270.2.
DR AlphaFoldDB; Q6NYV8; -.
DR Ensembl; ENSDART00000161098; ENSDARP00000138738; ENSDARG00000103320.
DR GeneID; 406554; -.
DR KEGG; dre:406554; -.
DR CTD; 253558; -.
DR ZFIN; ZDB-GENE-081211-2; lclat1.
DR GeneTree; ENSGT00950000182836; -.
DR HOGENOM; CLU_041844_4_0_1; -.
DR InParanoid; Q6NYV8; -.
DR OMA; YWTVFPL; -.
DR OrthoDB; 959325at2759; -.
DR PhylomeDB; Q6NYV8; -.
DR Reactome; R-DRE-1482798; Acyl chain remodeling of CL.
DR Reactome; R-DRE-1483166; Synthesis of PA.
DR UniPathway; UPA00557; UER00613.
DR PRO; PR:Q6NYV8; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 13.
DR Bgee; ENSDARG00000103320; Expressed in intestine and 23 other tissues.
DR GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0003841; F:1-acylglycerol-3-phosphate O-acyltransferase activity; ISS:UniProtKB.
DR GO; GO:0016746; F:acyltransferase activity; IBA:GO_Central.
DR GO; GO:0016024; P:CDP-diacylglycerol biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0035162; P:embryonic hemopoiesis; IDA:ZFIN.
DR GO; GO:0001885; P:endothelial cell development; IMP:ZFIN.
DR GO; GO:0060217; P:hemangioblast cell differentiation; IMP:ZFIN.
DR GO; GO:0036149; P:phosphatidylinositol acyl-chain remodeling; IBA:GO_Central.
DR InterPro; IPR032098; Acyltransf_C.
DR InterPro; IPR002123; Plipid/glycerol_acylTrfase.
DR Pfam; PF16076; Acyltransf_C; 1.
DR Pfam; PF01553; Acyltransferase; 1.
DR SMART; SM00563; PlsC; 1.
PE 1: Evidence at protein level;
KW Acyltransferase; Developmental protein; Endoplasmic reticulum;
KW Lipid biosynthesis; Lipid metabolism; Membrane; Phospholipid biosynthesis;
KW Phospholipid metabolism; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..388
FT /note="Lysocardiolipin acyltransferase 1"
FT /id="PRO_0000291580"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 321..341
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOTIF 85..90
FT /note="HXXXXD motif"
FT /evidence="ECO:0000250|UniProtKB:Q9D517"
FT MUTAGEN 165
FT /note="E->R: Abolishes function in hematopoietic
FT development; when associated with L-166."
FT /evidence="ECO:0000269|PubMed:18388326"
FT MUTAGEN 166
FT /note="G->L: Abolishes function in hematopoietic
FT development; when associated with R-165."
FT /evidence="ECO:0000269|PubMed:18388326"
SQ SEQUENCE 388 AA; 44745 MW; 005A38750BDB1B0E CRC64;
MVSPRGVCFL LFLLLGSVFG SVFMLGPLLP LMLLSPSRYR WITDRIVATW LTLPVALLEL
VLGVKVVVTG DGFIPGERSV IIMNHRTRLD WMFLWCCLLR YSYLRQEKIC LKAALKSVPG
FGWAMQVASF IFIQRRWEDD RTHMSNMLQY FCRIREPVQL LLFPEGTDLT ENTRARSDEF
AEKNGLQKYE YVLHPRTTGF TFIVDTLRGG DNLDAVHDIT VAYPQNIPQT ERHLLAGVFP
REIHFHVQRF TVASVPAGAA GLQAWCQERW REKERRLQRF YETVPRRFDA PAVGVCVREP
CCQSGQCVCV PRCKSEGRVR IILVASLLYW SVFITAACAS LCLCPPAQFY FLFMVVFFLC
QQRFTGGLEL MELACHRYWS RRSADKQE
