LCLT1_MOUSE
ID LCLT1_MOUSE Reviewed; 376 AA.
AC Q3UN02;
DT 26-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2007, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Lysocardiolipin acyltransferase 1;
DE EC=2.3.1.- {ECO:0000269|PubMed:15152008};
DE AltName: Full=1-acylglycerol-3-phosphate O-acyltransferase 8 {ECO:0000250|UniProtKB:Q6UWP7};
DE Short=1-AGP acyltransferase 8;
DE Short=1-AGPAT 8;
DE EC=2.3.1.51 {ECO:0000250|UniProtKB:Q6UWP7};
DE AltName: Full=Acyl-CoA:lysocardiolipin acyltransferase 1 {ECO:0000303|PubMed:15152008};
GN Name=Lclat1;
GN Synonyms=Agpat8 {ECO:0000250|UniProtKB:Q6UWP7},
GN Alcat1 {ECO:0000303|PubMed:15152008}, Gm91, Lycat;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-281.
RC TISSUE=Lung;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=15152008; DOI=10.1074/jbc.m402930200;
RA Cao J., Liu Y., Lockwood J., Burn P., Shi Y.;
RT "A novel cardiolipin-remodeling pathway revealed by a gene encoding an
RT endoplasmic reticulum-associated acyl-CoA:lysocardiolipin acyltransferase
RT (ALCAT1) in mouse.";
RL J. Biol. Chem. 279:31727-31734(2004).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=16620771; DOI=10.1016/j.abb.2006.03.014;
RA Agarwal A.K., Barnes R.I., Garg A.;
RT "Functional characterization of human 1-acylglycerol-3-phosphate
RT acyltransferase isoform 8: cloning, tissue distribution, gene structure,
RT and enzymatic activity.";
RL Arch. Biochem. Biophys. 449:64-76(2006).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=17675553; DOI=10.1182/blood-2007-04-086827;
RA Wang C., Faloon P.W., Tan Z., Lv Y., Zhang P., Ge Y., Deng H., Xiong J.-W.;
RT "Mouse lysocardiolipin acyltransferase controls the development of
RT hematopoietic and endothelial lineages during in vitro embryonic stem-cell
RT differentiation.";
RL Blood 110:3601-3609(2007).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [7]
RP FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=20668164; DOI=10.1091/mbc.e10-03-0195;
RA Imae R., Inoue T., Kimura M., Kanamori T., Tomioka N.H., Kage-Nakadai E.,
RA Mitani S., Arai H.;
RT "Intracellular phospholipase A1 and acyltransferase, which are involved in
RT Caenorhabditis elegans stem cell divisions, determine the sn-1 fatty acyl
RT chain of phosphatidylinositol.";
RL Mol. Biol. Cell 21:3114-3124(2010).
CC -!- FUNCTION: Exhibits acyl-CoA:lysocardiolipin acyltransferase (ALCAT)
CC activity; catalyzes the reacylation of lyso-cardiolipin to cardiolipin
CC (CL), a key step in CL remodeling (PubMed:15152008). Recognizes both
CC monolysocardiolipin and dilysocardiolipin as substrates with a
CC preference for linoleoyl-CoA and oleoyl-CoA as acyl donors
CC (PubMed:15152008). Also exhibits 1-acyl-sn-glycerol-3-phosphate
CC acyltransferase activity (AGPAT) activity; converts 1-acyl-sn-glycerol-
CC 3- phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-
CC glycerol-3- phosphate (phosphatidic acid or PA) by incorporating an
CC acyl moiety at the sn-2 position of the glycerol backbone (By
CC similarity). Possesses lysophosphatidylinositol acyltransferase (LPIAT)
CC activity (PubMed:20668164). Possesses lysophosphatidylglycerol
CC acyltransferase (LPGAT) activity (By similarity). Required for
CC establishment of the hematopoietic and endothelial lineages
CC (PubMed:17675553). {ECO:0000250|UniProtKB:Q6UWP7,
CC ECO:0000269|PubMed:15152008, ECO:0000269|PubMed:17675553,
CC ECO:0000269|PubMed:20668164}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-
CC sn-glycero-3-phosphate + CoA; Xref=Rhea:RHEA:19709,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57970, ChEBI:CHEBI:58342,
CC ChEBI:CHEBI:58608; EC=2.3.1.51;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19710;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1-acyl-sn-glycero-3-phospho-(1D-myo-inositol) + an acyl-CoA
CC = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + CoA;
CC Xref=Rhea:RHEA:33195, ChEBI:CHEBI:57287, ChEBI:CHEBI:57880,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64771;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33196;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-acyl-sn-glycero-3-phospho-(1'-sn-glycerol) + an acyl-CoA =
CC 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + CoA;
CC Xref=Rhea:RHEA:33203, ChEBI:CHEBI:57287, ChEBI:CHEBI:58342,
CC ChEBI:CHEBI:64716, ChEBI:CHEBI:64840;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33204;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-hexadecanoyl-sn-glycero-3-phosphate
CC = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA;
CC Xref=Rhea:RHEA:33187, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC ChEBI:CHEBI:57518, ChEBI:CHEBI:64839;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33188;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-
CC phosphate = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA;
CC Xref=Rhea:RHEA:37131, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC ChEBI:CHEBI:74544, ChEBI:CHEBI:74546;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37132;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-(9Z,12Z)-octadecadienoyl-sn-glycero-
CC 3-phosphate = 1-(9Z,12Z)-octadecadienoyl-2-(9Z)-octadecenoyl-sn-
CC glycero-3-phosphate + CoA; Xref=Rhea:RHEA:37135, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57387, ChEBI:CHEBI:74547, ChEBI:CHEBI:74548;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37136;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-(9Z,12Z,15Z)-octadecatrienoyl-sn-
CC glycero-3-phosphate = 1-(9Z,12Z,15Z)-octadecatrienoyl-2-(9Z)-
CC octadecenoyl-sn-glycero-3-phosphate + CoA; Xref=Rhea:RHEA:37139,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:74549,
CC ChEBI:CHEBI:74550; Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37140;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + hexadecanoyl-CoA
CC = 1-(9Z)-octadecenoyl-2-hexadecanoyl-sn-glycero-3-phosphate + CoA;
CC Xref=Rhea:RHEA:37143, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379,
CC ChEBI:CHEBI:74544, ChEBI:CHEBI:74551;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37144;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + octadecanoyl-CoA
CC = 1-(9Z-octadecenoyl)-2-octadecanoyl-sn-glycero-3-phosphate + CoA;
CC Xref=Rhea:RHEA:37147, ChEBI:CHEBI:57287, ChEBI:CHEBI:57394,
CC ChEBI:CHEBI:74544, ChEBI:CHEBI:74552;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37148;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-acyl-sn-glycero-3-phospho-(1'-sn-
CC glycerol) = 1-acyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-
CC glycerol) + CoA; Xref=Rhea:RHEA:37619, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57387, ChEBI:CHEBI:64840, ChEBI:CHEBI:75173;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37620;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + a 1-acyl-sn-glycero-3-phospho-(1D-myo-
CC inositol) = a 1-acyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1D-
CC myo-inositol) + CoA; Xref=Rhea:RHEA:37623, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57387, ChEBI:CHEBI:64771, ChEBI:CHEBI:75116;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37624;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-hexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol) +
CC hexadecanoyl-CoA = 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1D-myo-
CC inositol) + CoA; Xref=Rhea:RHEA:35871, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57379, ChEBI:CHEBI:72833, ChEBI:CHEBI:72835;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35872;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-hexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol) +
CC octadecanoyl-CoA = 1-hexadecanoyl-2-octadecanoyl-sn-glycero-3-
CC phospho-(1D-myo-inositol) + CoA; Xref=Rhea:RHEA:35875,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57394, ChEBI:CHEBI:72833,
CC ChEBI:CHEBI:72836; Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35876;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-hexadecanoyl-sn-glycero-3-phospho-
CC (1D-myo-inositol) = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-
CC phospho-(1D-myo-inositol) + CoA; Xref=Rhea:RHEA:35879,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:72833,
CC ChEBI:CHEBI:72837; Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35880;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z,12Z)-octadecadienoyl-CoA + 1-hexadecanoyl-sn-glycero-3-
CC phospho-(1D-myo-inositol) = 1-hexadecanoyl-2-(9Z,12Z-
CC octadecadienoyl)-sn-glycero-3-phospho-(1D-myo-inositol) + CoA;
CC Xref=Rhea:RHEA:35883, ChEBI:CHEBI:57287, ChEBI:CHEBI:57383,
CC ChEBI:CHEBI:72833, ChEBI:CHEBI:72838;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35884;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 1-hexadecanoyl-sn-
CC glycero-3-phospho-(1D-myo-inositol) = 1-hexadecanoyl-2-
CC (5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phospho-D-myo-inositol
CC + CoA; Xref=Rhea:RHEA:35867, ChEBI:CHEBI:57287, ChEBI:CHEBI:57368,
CC ChEBI:CHEBI:72833, ChEBI:CHEBI:72834;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35868;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-hexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) +
CC hexadecanoyl-CoA = 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1'-sn-
CC glycerol) + CoA; Xref=Rhea:RHEA:35851, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57379, ChEBI:CHEBI:72829, ChEBI:CHEBI:75158;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35852;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-hexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) +
CC octadecanoyl-CoA = 1-hexadecanoyl-2-octadecanoyl-sn-glycero-3-
CC phospho-(1'-sn-glycerol) + CoA; Xref=Rhea:RHEA:35887,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57394, ChEBI:CHEBI:72839,
CC ChEBI:CHEBI:75158; Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35888;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-hexadecanoyl-sn-glycero-3-phospho-
CC (1'-sn-glycerol) = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-
CC phospho-(1'-sn-glycerol) + CoA; Xref=Rhea:RHEA:35891,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:72841,
CC ChEBI:CHEBI:75158; Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35892;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z,12Z)-octadecadienoyl-CoA + 1-hexadecanoyl-sn-glycero-3-
CC phospho-(1'-sn-glycerol) = 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-
CC sn-glycero-3-phospho-(1'-sn-glycerol) + CoA; Xref=Rhea:RHEA:35895,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57383, ChEBI:CHEBI:72840,
CC ChEBI:CHEBI:75158; Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:35896;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-tetradecanoyl-sn-glycero-3-phospho-
CC (1'-sn-glycerol) = 1-tetradecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-
CC phospho-(1'-sn-glycerol) + CoA; Xref=Rhea:RHEA:37643,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:72826,
CC ChEBI:CHEBI:75161; Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37644;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-octadecanoyl-sn-glycero-3-phospho-
CC (1'-sn-glycerol) = 1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-
CC phospho-(1'-sn-glycerol) + CoA; Xref=Rhea:RHEA:37647,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:72827,
CC ChEBI:CHEBI:72845; Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37648;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-sn-glycero-3-
CC phospho-(1'-sn-glycerol) = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-
CC phospho-(1'-sn-glycerol) + CoA; Xref=Rhea:RHEA:37651,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:72828,
CC ChEBI:CHEBI:75163; Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37652;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-hexadecanoyl-sn-glycero-3-phospho-(1D-myo-inositol) +
CC dodecanoyl-CoA = 1-hexadecanoyl-2-dodecanoyl-sn-glycero-3-phospho-
CC (1D-myo-inositol) + CoA; Xref=Rhea:RHEA:37639, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57375, ChEBI:CHEBI:72833, ChEBI:CHEBI:75160;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37640;
CC Evidence={ECO:0000250|UniProtKB:Q6UWP7};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1',3'-bis-[1-acyl-sn-glycero-3-
CC phospho]-glycerol = 1'-[1-acyl-2-(9Z)-octadecenoyl-sn-glycero-3-
CC phospho],3'-[1-acyl,2-hydroxy-sn-glycero-3-phospho]-glycerol + CoA;
CC Xref=Rhea:RHEA:37615, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC ChEBI:CHEBI:75137, ChEBI:CHEBI:75139;
CC Evidence={ECO:0000269|PubMed:15152008};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37616;
CC Evidence={ECO:0000305|PubMed:15152008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1'-[1,2-diacyl-sn-glycero-3-
CC phospho],3'-[1-acyl-sn-glycero-3-phospho]-glycerol = 1'-[1,2-diacyl-
CC sn-glycero-3-phospho],3'-[1-acyl,2-(9Z)-octadecenoyl-sn-glycero-3-
CC phospho]-glycerol + CoA; Xref=Rhea:RHEA:37611, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57387, ChEBI:CHEBI:64743, ChEBI:CHEBI:75140;
CC Evidence={ECO:0000269|PubMed:15152008};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37612;
CC Evidence={ECO:0000305|PubMed:15152008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z,12Z)-octadecadienoyl-CoA + 1'-[1,2-diacyl-sn-glycero-3-
CC phospho],3'-[1-acyl-sn-glycero-3-phospho]-glycerol = 1'-[1,2-diacyl-
CC sn-glycero-3-phospho],3'-[1-acyl,2-(9Z,12Z)-octadecadienoyl-sn-
CC glycero-3-phospho]-glycerol + CoA; Xref=Rhea:RHEA:37675,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57383, ChEBI:CHEBI:64743,
CC ChEBI:CHEBI:75205; Evidence={ECO:0000269|PubMed:15152008};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37676;
CC Evidence={ECO:0000305|PubMed:15152008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1'-[1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl-sn-glycero-3-
CC phospho]-glycerol + dodecanoyl-CoA = 1'-[1,2-diacyl-sn-glycero-3-
CC phospho],3'-[1-acyl,2-dodecanoyl-sn-glycero-3-phospho]-glycerol +
CC CoA; Xref=Rhea:RHEA:37679, ChEBI:CHEBI:57287, ChEBI:CHEBI:57375,
CC ChEBI:CHEBI:64743, ChEBI:CHEBI:75203;
CC Evidence={ECO:0000269|PubMed:15152008};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37680;
CC Evidence={ECO:0000305|PubMed:15152008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1',3'-bis-[1-acyl-sn-glycero-3-phospho]-glycerol + dodecanoyl-
CC CoA = 1'-[1-acyl-2-dodecanoyl-sn-glycero-3-phospho],3'-[1-acyl,2-
CC hydroxy-sn-glycero-3-phospho]-glycerol + CoA; Xref=Rhea:RHEA:37683,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57375, ChEBI:CHEBI:75137,
CC ChEBI:CHEBI:75201; Evidence={ECO:0000269|PubMed:15152008};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37684;
CC Evidence={ECO:0000305|PubMed:15152008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + a 1-acyl-sn-glycero-3-phosphate = a 1-
CC acyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CoA;
CC Xref=Rhea:RHEA:37427, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC ChEBI:CHEBI:57970, ChEBI:CHEBI:74917;
CC Evidence={ECO:0000269|PubMed:15152008};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37428;
CC Evidence={ECO:0000305|PubMed:15152008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z,12Z)-octadecadienoyl-CoA + 1',3'-bis-[1-acyl-sn-glycero-3-
CC phospho]-glycerol = 1'-[1-acyl-2-(9Z,12Z)-octadecadienoyl-sn-glycero-
CC 3-phospho],3'-[1-acyl,2-hydroxy-sn-glycero-3-phospho]-glycerol + CoA;
CC Xref=Rhea:RHEA:37687, ChEBI:CHEBI:57287, ChEBI:CHEBI:57383,
CC ChEBI:CHEBI:75137, ChEBI:CHEBI:75209;
CC Evidence={ECO:0000269|PubMed:15152008};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37688;
CC Evidence={ECO:0000305|PubMed:15152008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1',3'-bis-[1-acyl-sn-glycero-3-phospho]-glycerol +
CC hexadecanoyl-CoA = 1'-[1-acyl-2-hexadecanoyl-sn-glycero-3-
CC phospho],3'-[1-acyl,2-hydroxy-sn-glycero-3-phospho]-glycerol + CoA;
CC Xref=Rhea:RHEA:37691, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379,
CC ChEBI:CHEBI:75137, ChEBI:CHEBI:75207;
CC Evidence={ECO:0000269|PubMed:15152008};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37692;
CC Evidence={ECO:0000305|PubMed:15152008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1',3'-bis-[1-acyl-sn-glycero-3-phospho]-glycerol +
CC octadecanoyl-CoA = 1'-[1-acyl-2-octadecanoyl-sn-glycero-3-
CC phospho],3'-[1-acyl,2-hydroxy-sn-glycero-3-phospho]-glycerol + CoA;
CC Xref=Rhea:RHEA:37695, ChEBI:CHEBI:57287, ChEBI:CHEBI:57394,
CC ChEBI:CHEBI:75137, ChEBI:CHEBI:75208;
CC Evidence={ECO:0000269|PubMed:15152008};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37696;
CC Evidence={ECO:0000305|PubMed:15152008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1'-[1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl-sn-glycero-3-
CC phospho]-glycerol + octanoyl-CoA = 1'-[1,2-diacyl-sn-glycero-3-
CC phospho],3'-[1-acyl,2-octanoyl-sn-glycero-3-phospho]-glycerol + CoA;
CC Xref=Rhea:RHEA:38623, ChEBI:CHEBI:57287, ChEBI:CHEBI:57386,
CC ChEBI:CHEBI:64743, ChEBI:CHEBI:75990;
CC Evidence={ECO:0000269|PubMed:15152008};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38624;
CC Evidence={ECO:0000305|PubMed:15152008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1',3'-bis-[1-acyl-sn-glycero-3-phospho]-glycerol + octanoyl-
CC CoA = 1'-[1-acyl-2-octanoyl-sn-glycero-3-phospho],3'-[1-acyl,2-
CC hydroxy-sn-glycero-3-phospho]-glycerol + CoA; Xref=Rhea:RHEA:38627,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57386, ChEBI:CHEBI:75137,
CC ChEBI:CHEBI:75993; Evidence={ECO:0000269|PubMed:15152008};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38628;
CC Evidence={ECO:0000305|PubMed:15152008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1'-[1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl-sn-glycero-3-
CC phospho]-glycerol + hexadecanoyl-CoA = 1'-[1,2-diacyl-sn-glycero-3-
CC phospho],3'-[1-acyl,2-hexadecanoyl-sn-glycero-3-phospho]-glycerol +
CC CoA; Xref=Rhea:RHEA:38631, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379,
CC ChEBI:CHEBI:64743, ChEBI:CHEBI:75994;
CC Evidence={ECO:0000269|PubMed:15152008};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38632;
CC Evidence={ECO:0000305|PubMed:15152008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 1'-[1,2-diacyl-sn-
CC glycero-3-phospho],3'-[1-acyl-sn-glycero-3-phospho]-glycerol = 1'-
CC [1,2-diacyl-sn-glycero-3-phospho],3'-[1-acyl,2-(5Z,8Z,11Z,14Z)-
CC eicosatetraenoyl-sn-glycero-3-phospho]-glycerol + CoA;
CC Xref=Rhea:RHEA:38635, ChEBI:CHEBI:57287, ChEBI:CHEBI:57368,
CC ChEBI:CHEBI:64743, ChEBI:CHEBI:75995;
CC Evidence={ECO:0000269|PubMed:15152008};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38636;
CC Evidence={ECO:0000305|PubMed:15152008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 1',3'-bis-[1-acyl-sn-
CC glycero-3-phospho]-glycerol = 1'-[1-acyl-2-(5Z,8Z,11Z,14Z)-
CC eicosatetraenoyl-sn-glycero-3-phospho],3'-[1-acyl,2-hydroxy-sn-
CC glycero-3-phospho]-glycerol + CoA; Xref=Rhea:RHEA:38639,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57368, ChEBI:CHEBI:75137,
CC ChEBI:CHEBI:75996; Evidence={ECO:0000269|PubMed:15152008};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38640;
CC Evidence={ECO:0000305|PubMed:15152008};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1-acyl-sn-glycero-3-phospho-(1D-myo-inositol) +
CC octadecanoyl-CoA = a 1-acyl-2-octadecanoyl-sn-glycero-3-phospho-(1D-
CC myo-inositol) + CoA; Xref=Rhea:RHEA:43960, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57394, ChEBI:CHEBI:64771, ChEBI:CHEBI:83939;
CC Evidence={ECO:0000269|PubMed:20668164};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43961;
CC Evidence={ECO:0000305|PubMed:20668164};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2-acyl-sn-glycero-3-phospho-D-myo-inositol + octadecanoyl-
CC CoA = 1-octadecanoyl-2-acyl-sn-glycero-3-phospho-(1D-myo-inositol) +
CC CoA; Xref=Rhea:RHEA:43964, ChEBI:CHEBI:57287, ChEBI:CHEBI:57394,
CC ChEBI:CHEBI:64872, ChEBI:CHEBI:83940;
CC Evidence={ECO:0000269|PubMed:20668164};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43965;
CC Evidence={ECO:0000305|PubMed:20668164};
CC -!- PATHWAY: Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-
CC diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:15152008}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Widely expressed with highest expression in heart,
CC liver and 12.5 dpc aorta-gonad-mesonephros and lower levels in the 16
CC dpc fetal liver and adult bone marrow. In bone marrow, highest levels
CC are found in B-cells compared with whole bone marrow, T-cells,
CC erythrocytes, and granulocytes. {ECO:0000269|PubMed:15152008,
CC ECO:0000269|PubMed:16620771, ECO:0000269|PubMed:17675553}.
CC -!- DOMAIN: The HXXXXD motif is essential for acyltransferase activity and
CC may constitute the binding site for the phosphate moiety of the
CC glycerol-3-phosphate. {ECO:0000250|UniProtKB:Q9D517}.
CC -!- SIMILARITY: Belongs to the 1-acyl-sn-glycerol-3-phosphate
CC acyltransferase family. {ECO:0000305}.
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DR EMBL; AC112949; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK144580; BAE25946.1; -; mRNA.
DR CCDS; CCDS37689.1; -.
DR RefSeq; NP_001074540.1; NM_001081071.2.
DR RefSeq; NP_001171438.1; NM_001177967.1.
DR RefSeq; NP_001171439.1; NM_001177968.1.
DR RefSeq; XP_011244712.1; XM_011246410.2.
DR AlphaFoldDB; Q3UN02; -.
DR BioGRID; 230349; 4.
DR STRING; 10090.ENSMUSP00000068690; -.
DR SwissLipids; SLP:000000291; -.
DR GlyGen; Q3UN02; 1 site.
DR iPTMnet; Q3UN02; -.
DR PhosphoSitePlus; Q3UN02; -.
DR EPD; Q3UN02; -.
DR jPOST; Q3UN02; -.
DR MaxQB; Q3UN02; -.
DR PaxDb; Q3UN02; -.
DR PRIDE; Q3UN02; -.
DR ProteomicsDB; 265048; -.
DR Antibodypedia; 28989; 98 antibodies from 20 providers.
DR Ensembl; ENSMUST00000067545; ENSMUSP00000068690; ENSMUSG00000054469.
DR GeneID; 225010; -.
DR KEGG; mmu:225010; -.
DR UCSC; uc008dnh.2; mouse.
DR CTD; 253558; -.
DR MGI; MGI:2684937; Lclat1.
DR VEuPathDB; HostDB:ENSMUSG00000054469; -.
DR eggNOG; KOG1505; Eukaryota.
DR GeneTree; ENSGT00950000182836; -.
DR HOGENOM; CLU_041844_4_0_1; -.
DR InParanoid; Q3UN02; -.
DR OMA; YWTVFPL; -.
DR OrthoDB; 959325at2759; -.
DR PhylomeDB; Q3UN02; -.
DR TreeFam; TF314346; -.
DR BRENDA; 2.3.1.23; 3474.
DR BRENDA; 2.3.1.62; 3474.
DR Reactome; R-MMU-1482798; Acyl chain remodeling of CL.
DR Reactome; R-MMU-1483166; Synthesis of PA.
DR UniPathway; UPA00557; UER00613.
DR BioGRID-ORCS; 225010; 3 hits in 75 CRISPR screens.
DR ChiTaRS; Lclat1; mouse.
DR PRO; PR:Q3UN02; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q3UN02; protein.
DR Bgee; ENSMUSG00000054469; Expressed in epithelium of small intestine and 259 other tissues.
DR ExpressionAtlas; Q3UN02; baseline and differential.
DR Genevisible; Q3UN02; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:MGI.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0003841; F:1-acylglycerol-3-phosphate O-acyltransferase activity; ISS:UniProtKB.
DR GO; GO:0016746; F:acyltransferase activity; IDA:MGI.
DR GO; GO:0035965; P:cardiolipin acyl-chain remodeling; TAS:UniProtKB.
DR GO; GO:0016024; P:CDP-diacylglycerol biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0036149; P:phosphatidylinositol acyl-chain remodeling; IBA:GO_Central.
DR InterPro; IPR032098; Acyltransf_C.
DR InterPro; IPR002123; Plipid/glycerol_acylTrfase.
DR Pfam; PF16076; Acyltransf_C; 1.
DR Pfam; PF01553; Acyltransferase; 1.
DR SMART; SM00563; PlsC; 1.
PE 1: Evidence at protein level;
KW Acetylation; Acyltransferase; Developmental protein; Endoplasmic reticulum;
KW Glycoprotein; Lipid biosynthesis; Lipid metabolism; Membrane;
KW Phospholipid biosynthesis; Phospholipid metabolism; Reference proteome;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..376
FT /note="Lysocardiolipin acyltransferase 1"
FT /id="PRO_0000291578"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 309..329
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 336..356
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOTIF 85..90
FT /note="HXXXXD motif"
FT /evidence="ECO:0000250|UniProtKB:Q9D517"
FT MOD_RES 183
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q6UWP7"
FT CARBOHYD 35
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 376 AA; 44400 MW; C2E7AAC40145CD7E CRC64;
MVSWKGIYFI LFLFAGSFFG SIFMLGPILP LMFINLSWYR WISSRLVATW LTLPVALLET
MFGVRVVITG DAFVPGERSV IIMNHRTRVD WMFLWNCLMR YSYLRVEKIC LKSSLKSVPG
FGWAMQVAAF IFIHRKWKDD KSHFEDMIDY FCAIHEPLQL LIFPEGTDLT ENNKARSNDF
AEKNGLQKYE YVLHPRTTGF TFVVDRLREG KNLDAVHDIT VAYPYNIPQT EKHLLLGDFP
KEIHFHVQRY PADSLPTSKE DLQLWCHRRW EEKEERLRSF YQGEKNFHFT GQSTVPPCKS
ELRVLVVKLL SIVYWALFCS AMCLLIYLYS PVRWYFIISI VFFVLQERIF GGLEIIELAC
YRFLHKHPHL NSKKNE
