ID   LCNB_LACLC              Reviewed;          68 AA.
AC   P35518;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Bacteriocin lactococcin-B;
DE            Short=LCN-B;
DE   Flags: Precursor;
GN   Name=lcnB;
OS   Lactococcus lactis subsp. cremoris (Streptococcus cremoris).
OG   Plasmid p9B4-6.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=1359;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=9B4;
RX   PubMed=1610182; DOI=10.1128/aem.58.2.572-577.1992;
RA   van Belkum M.J., Kok J., Venema G.;
RT   "Cloning, sequencing, and expression in Escherichia coli of lcnB, a third
RT   bacteriocin determinant from the lactococcal bacteriocin plasmid p9B4-6.";
RL   Appl. Environ. Microbiol. 58:572-577(1992).
RN   [2]
RP   MUTAGENESIS OF CYS-45.
RX   PubMed=8885398; DOI=10.1099/13500872-142-10-2825;
RA   Venema K., Dost M.H., Venema G., Kok J.;
RT   "Mutational analysis and chemical modification of Cys24 of lactococcin B, a
RT   bacteriocin produced by Lactococcus lactis.";
RL   Microbiology 142:2825-2830(1996).
CC   -!- FUNCTION: Kills Lactococci by dissipating the membrane potential of the
CC       cells.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; S38128; AAB22372.1; -; Genomic_DNA.
DR   PIR; B43940; B43940.
DR   RefSeq; WP_015081788.1; NZ_WJUX01000070.1.
DR   AlphaFoldDB; P35518; -.
DR   TCDB; 1.C.22.1.2; the lactococcin a (lactococcin a) family.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR007464; Bacteriocin_IId.
DR   InterPro; IPR010133; Bacteriocin_signal_seq.
DR   Pfam; PF04369; Lactococcin; 1.
DR   TIGRFAMs; TIGR01847; bacteriocin_sig; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Bacteriocin; Plasmid; Secreted.
FT   PROPEP          1..21
FT                   /id="PRO_0000002770"
FT   CHAIN           22..68
FT                   /note="Bacteriocin lactococcin-B"
FT                   /id="PRO_0000002771"
FT   MUTAGEN         45
FT                   /note="C->A,D,E,F,G,I,L,M,N,P,Q,S,T,V,Y,W: No loss of
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:8885398"
FT   MUTAGEN         45
FT                   /note="C->R,K: Loss of activity."
FT                   /evidence="ECO:0000269|PubMed:8885398"
SQ   SEQUENCE   68 AA;  7632 MW;  18382310AC880678 CRC64;
     MKNQLNFNIV SDEELAEVNG GSLQYVMSAG PYTWYKDTRT GKTICKQTID TASYTFGVMA
     EGWGKTFH