ID   LCND_LACLC              Reviewed;         474 AA.
AC   P0A3G6; Q00565;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Lactococcin A secretion protein LcnD;
GN   Name=lcnD;
OS   Lactococcus lactis subsp. cremoris (Streptococcus cremoris).
OG   Plasmid p9B4-6.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=1359;
RN   [1]
RP   PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=9B4; PLASMID=p9B4-6;
RX   PubMed=1901707; DOI=10.1128/aem.57.2.492-498.1991;
RA   van Belkum M.J., Hayema B.J., Jeeninga R.E., Kok J., Venema G.;
RT   "Organization and nucleotide sequences of two lactococcal bacteriocin
RT   operons.";
RL   Appl. Environ. Microbiol. 57:492-498(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 421-474.
RC   STRAIN=LMG 2130;
RX   PubMed=1904860; DOI=10.1128/jb.173.12.3879-3887.1991;
RA   Holo H., Nilssen O., Nes I.F.;
RT   "Lactococcin A, a new bacteriocin from Lactococcus lactis subsp. cremoris:
RT   isolation and characterization of the protein and its gene.";
RL   J. Bacteriol. 173:3879-3887(1991).
CC   -!- FUNCTION: Involved in the secretion of lactococcin A.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane
CC       protein.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
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DR   PIR; C39443; C39443.
DR   RefSeq; WP_015081785.1; NZ_WJUX01000113.1.
DR   AlphaFoldDB; P0A3G6; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043213; P:bacteriocin transport; IEA:UniProtKB-KW.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   InterPro; IPR005696; MesE/LcnD.
DR   InterPro; IPR006144; Secretion_HlyD_CS.
DR   TIGRFAMs; TIGR01000; bacteriocin_acc; 1.
DR   PROSITE; PS00543; HLYD_FAMILY; 1.
PE   3: Inferred from homology;
KW   Bacteriocin transport; Cell membrane; Membrane; Plasmid; Protein transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..474
FT                   /note="Lactococcin A secretion protein LcnD"
FT                   /id="PRO_0000201879"
FT   TOPO_DOM        1..21
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        22..44
FT                   /note="Helical"
FT                   /evidence="ECO:0000305"
FT   TOPO_DOM        45..474
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   474 AA;  52538 MW;  AA4151B8648F4A9D CRC64;
     MFDKKLLESS ELYDKRYRNF STLIILPLFI LLVGGVIFTF FAHKELTVIS TGSIEPTKIV
     AKIQSTNANP IIENNLKEGE AVKENSLLLK YNGTPEQTQL SELLTQKKQA LDKKVQLDLL
     QRSLTNEKNE FPTADSFGYE KSFENYEAQV KSLEATIQKS NQAVEDQNKS TESQKQAIQN
     QVATLQQAIQ NYSEIENAVS SGGGVSQDNP YLSQYNSYQA QQATLEADLK NQKNPDETAK
     QAAKSQEESL KSQFLSGLAS SKDSLKSQIQ SFNVQESSLT GSNAYDNSQS SQILTLKSQA
     LSASNKEMTD LNSTLTDLET KISLQKQDDQ YSQVFAEQAG VLHVLPDILG MKKIPIGTPI
     AEIYPLLKSE TQVNLTSYIP STQISGMKVG QKVRFTVQQN LPQPEILTGI INQIDSAPTA
     FKEGNAYKVS ATTTINAKDL PNIRYGLQGK TVTIIGKKTY FNYFLDKIMG RGNQ