ID   LCND_LACLL              Reviewed;         474 AA.
AC   P0A3G5; Q00565;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=Lactococcin A secretion protein LcnD;
GN   Name=lcnD;
OS   Lactococcus lactis subsp. lactis (Streptococcus lactis).
OG   Plasmid pNP2.
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=1360;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Biovar diacetylactis WM4; PLASMID=pNP2;
RX   PubMed=1622271; DOI=10.1128/aem.58.6.1952-1961.1992;
RA   Stoddard G.W., Petzel J.P., van Belkum M.J., Kok J., McKay L.L.;
RT   "Molecular analyses of the lactococcin A gene cluster from Lactococcus
RT   lactis subsp. lactis biovar diacetylactis WM4.";
RL   Appl. Environ. Microbiol. 58:1952-1961(1992).
RN   [2]
RP   TOPOLOGY.
RX   PubMed=8626308; DOI=10.1128/jb.178.6.1766-1769.1996;
RA   Franke C.M., Leenhouts K.J., Haandrikman A.J., Kok J., Venema G.,
RA   Venema K.;
RT   "Topology of LcnD, a protein implicated in the transport of bacteriocins
RT   from Lactococcus lactis.";
RL   J. Bacteriol. 178:1766-1769(1996).
CC   -!- FUNCTION: Involved in the secretion of lactococcin A.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane
CC       protein.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
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DR   EMBL; M90969; AAA92855.1; -; Genomic_DNA.
DR   PIR; D43943; D43943.
DR   RefSeq; WP_015081785.1; NZ_WJUU01000069.1.
DR   AlphaFoldDB; P0A3G5; -.
DR   TCDB; 3.A.1.112.3; the atp-binding cassette (abc) superfamily.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043213; P:bacteriocin transport; IEA:UniProtKB-KW.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   InterPro; IPR005696; MesE/LcnD.
DR   InterPro; IPR006144; Secretion_HlyD_CS.
DR   TIGRFAMs; TIGR01000; bacteriocin_acc; 1.
DR   PROSITE; PS00543; HLYD_FAMILY; 1.
PE   1: Evidence at protein level;
KW   Bacteriocin transport; Cell membrane; Membrane; Plasmid; Protein transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..474
FT                   /note="Lactococcin A secretion protein LcnD"
FT                   /id="PRO_0000201878"
FT   TOPO_DOM        1..21
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:8626308"
FT   TRANSMEM        22..44
FT                   /note="Helical"
FT                   /evidence="ECO:0000305"
FT   TOPO_DOM        45..474
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000305|PubMed:8626308"
SQ   SEQUENCE   474 AA;  52538 MW;  AA4151B8648F4A9D CRC64;
     MFDKKLLESS ELYDKRYRNF STLIILPLFI LLVGGVIFTF FAHKELTVIS TGSIEPTKIV
     AKIQSTNANP IIENNLKEGE AVKENSLLLK YNGTPEQTQL SELLTQKKQA LDKKVQLDLL
     QRSLTNEKNE FPTADSFGYE KSFENYEAQV KSLEATIQKS NQAVEDQNKS TESQKQAIQN
     QVATLQQAIQ NYSEIENAVS SGGGVSQDNP YLSQYNSYQA QQATLEADLK NQKNPDETAK
     QAAKSQEESL KSQFLSGLAS SKDSLKSQIQ SFNVQESSLT GSNAYDNSQS SQILTLKSQA
     LSASNKEMTD LNSTLTDLET KISLQKQDDQ YSQVFAEQAG VLHVLPDILG MKKIPIGTPI
     AEIYPLLKSE TQVNLTSYIP STQISGMKVG QKVRFTVQQN LPQPEILTGI INQIDSAPTA
     FKEGNAYKVS ATTTINAKDL PNIRYGLQGK TVTIIGKKTY FNYFLDKIMG RGNQ