ID   LCPA_CORGL              Reviewed;         518 AA.
AC   Q8NSD6; Q6M740;
DT   22-NOV-2017, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Cell wall biosynthesis protein LcpA {ECO:0000305};
GN   Name=lcpA {ECO:0000303|PubMed:27551018};
GN   OrderedLocusNames=Cgl0740 {ECO:0000312|EMBL:BAB98133.1};
OS   Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 /
OS   JCM 1318 / LMG 3730 / NCIMB 10025).
OC   Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
OC   Corynebacterium.
OX   NCBI_TaxID=196627;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC   10025;
RX   PubMed=12743753; DOI=10.1007/s00253-003-1328-1;
RA   Ikeda M., Nakagawa S.;
RT   "The Corynebacterium glutamicum genome: features and impacts on
RT   biotechnological processes.";
RL   Appl. Microbiol. Biotechnol. 62:99-109(2003).
RN   [2]
RP   FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, DOMAIN, DISRUPTION PHENOTYPE, AND
RP   MUTAGENESIS OF ASP-88; ARG-138 AND ARG-257.
RC   STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC   10025;
RX   PubMed=27551018; DOI=10.1128/jb.00406-16;
RA   Baumgart M., Schubert K., Bramkamp M., Frunzke J.;
RT   "Impact of LytR-CpsA-Psr proteins on cell wall biosynthesis in
RT   Corynebacterium glutamicum.";
RL   J. Bacteriol. 198:3045-3059(2016).
CC   -!- FUNCTION: Involved in cell wall biosynthesis. May be responsible for
CC       the transfer of arabinogalactan onto peptidoglycan. In vitro, has
CC       pyrophosphatase activity. {ECO:0000269|PubMed:27551018}.
CC   -!- SUBUNIT: Forms homodimers and homotetramers.
CC       {ECO:0000269|PubMed:27551018}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000269|PubMed:27551018}; Single-pass membrane protein
CC       {ECO:0000255}. Note=Localizes at regions of strong cell wall
CC       biosynthesis, such as the poles and the division plane.
CC       {ECO:0000269|PubMed:27551018}.
CC   -!- DOMAIN: Both the C-terminal LCP and LytR_C regions are essential for
CC       function. The N-terminal region is not strictly required.
CC       {ECO:0000269|PubMed:27551018}.
CC   -!- DISRUPTION PHENOTYPE: Essential, it cannot be deleted. Conditional
CC       silencing of the gene results in severe growth defects and drastic
CC       morphological changes. The lcpA-silencing strain contains significantly
CC       less mycolic acids and a reduced amount of arabinogalactan.
CC       {ECO:0000269|PubMed:27551018}.
CC   -!- SIMILARITY: Belongs to the LytR/CpsA/Psr (LCP) family. {ECO:0000305}.
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DR   EMBL; BA000036; BAB98133.1; -; Genomic_DNA.
DR   RefSeq; NP_599970.1; NC_003450.3.
DR   RefSeq; WP_011013861.1; NC_006958.1.
DR   AlphaFoldDB; Q8NSD6; -.
DR   STRING; 196627.cg0847; -.
DR   KEGG; cgl:Cgl0740; -.
DR   PATRIC; fig|196627.13.peg.726; -.
DR   eggNOG; COG1316; Bacteria.
DR   HOGENOM; CLU_016455_4_0_11; -.
DR   OMA; YVPKDMK; -.
DR   Proteomes; UP000000582; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   InterPro; IPR027381; LytR/CpsA/Psr_C.
DR   InterPro; IPR004474; LytR_CpsA_psr.
DR   Pfam; PF13399; LytR_C; 1.
DR   Pfam; PF03816; LytR_cpsA_psr; 1.
DR   TIGRFAMs; TIGR00350; lytR_cpsA_psr; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Cell wall biogenesis/degradation;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..518
FT                   /note="Cell wall biosynthesis protein LcpA"
FT                   /id="PRO_0000442346"
FT   TOPO_DOM        1..31
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305|PubMed:27551018"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        53..518
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000305|PubMed:27551018"
FT   REGION          485..518
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         88
FT                   /note="D->A: Does not complement the growth defect of the
FT                   lcpA-silencing strain."
FT                   /evidence="ECO:0000269|PubMed:27551018"
FT   MUTAGEN         138
FT                   /note="R->A: Does not complement the growth defect of the
FT                   lcpA-silencing strain."
FT                   /evidence="ECO:0000269|PubMed:27551018"
FT   MUTAGEN         257
FT                   /note="R->A: Does not complement the growth defect of the
FT                   lcpA-silencing strain."
FT                   /evidence="ECO:0000269|PubMed:27551018"
SQ   SEQUENCE   518 AA;  53916 MW;  C792B077ACA51522 CRC64;
     MTEKYRPVRD IKPAPAAMQS TKQAGHPVFR SVVAFVSVLV LLVSGLGYLA VGKVDGVASG
     NLNLGGGRGI QDGNAADGAT DILLVGSDSR SDAQGNTLTE EELAMLRAGD EENDNTDTIM
     VIRVPNDGSS ATAVSIPRDT YIHDDDYGNM KINGVYGAYK DARRAELMEQ GFTNESELET
     RAKDAGREGL IDAVSDLTGI TVDHYAEVGL LGFVLLTDAV GGVEVCLNNA VDEPLSGANF
     PAGRQTLGGS DALSYVRQRH DLPRGDLDRI VRQQSYMASL VNQVLSSGTL TNPAKLSALA
     DAVTRSVVID EGWEIMSFAT QLQNLAGGNV TFATIPVTSI DGTGDYGESV VTIDVNQVHA
     FFQEALGEAE PAPEDGSDDQ SADQAPDLSE VEVHVLNASY VEGLANGIAA QLQELGYSIA
     ETGNAAEGLY YESQILAAEE DSAKALAISE ALGGLPIVAN SSLDDNTVIV VSAGDYAGPT
     AEANAVTSST VGQPGADVGE PIESPEFDAG GDGPRCVN