LCPS_GIBFU
ID LCPS_GIBFU Reviewed; 612 AA.
AC Q8X0Z1;
DT 31-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 65.
DE RecName: Full=Bifunctional lycopene cyclase/phytoene synthase {ECO:0000303|PubMed:12172798};
DE Includes:
DE RecName: Full=Lycopene beta-cyclase {ECO:0000303|PubMed:12172798};
DE EC=5.5.1.19 {ECO:0000269|PubMed:12172798};
DE AltName: Full=Carotene cyclase {ECO:0000303|PubMed:12172798};
DE AltName: Full=Lycopene cyclase {ECO:0000303|PubMed:12172798};
DE Includes:
DE RecName: Full=Phytoene synthase {ECO:0000303|PubMed:12172798};
DE EC=2.5.1.32 {ECO:0000269|PubMed:12172798};
GN Name=carRA {ECO:0000303|PubMed:12172798};
OS Gibberella fujikuroi (Bakanae and foot rot disease fungus) (Fusarium
OS fujikuroi).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC Fusarium fujikuroi species complex.
OX NCBI_TaxID=5127;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, AND
RP INDUCTION.
RX PubMed=12172798; DOI=10.1007/s00438-002-0690-5;
RA Linnemannstons P., Prado M.M., Fernandez-Martin R., Tudzynski B.,
RA Avalos J.;
RT "A carotenoid biosynthesis gene cluster in Fusarium fujikuroi: the genes
RT carB and carRA.";
RL Mol. Genet. Genomics 267:593-602(2002).
CC -!- FUNCTION: Bifunctional enzyme that catalyzes the reactions from
CC geranylgeranyl diphosphate to phytoene and from lycopene to beta-
CC carotene via the intermediate gamma-carotene. Gamma-carotene is further
CC processed to the acidic carotenoid neurosporaxanthin.
CC {ECO:0000269|PubMed:12172798}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-lycopene = gamma-carotene; Xref=Rhea:RHEA:32219,
CC ChEBI:CHEBI:15948, ChEBI:CHEBI:27740; EC=5.5.1.19;
CC Evidence={ECO:0000269|PubMed:12172798};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=gamma-carotene = all-trans-beta-carotene;
CC Xref=Rhea:RHEA:32239, ChEBI:CHEBI:17579, ChEBI:CHEBI:27740;
CC EC=5.5.1.19; Evidence={ECO:0000269|PubMed:12172798};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2
CC diphosphate; Xref=Rhea:RHEA:34475, ChEBI:CHEBI:27787,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:58756; EC=2.5.1.32;
CC Evidence={ECO:0000269|PubMed:12172798};
CC -!- PATHWAY: Carotenoid biosynthesis; beta-carotene biosynthesis.
CC {ECO:0000269|PubMed:12172798}.
CC -!- PATHWAY: Carotenoid biosynthesis; phytoene biosynthesis; all-trans-
CC phytoene from geranylgeranyl diphosphate: step 1/1.
CC {ECO:0000269|PubMed:12172798}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Induced by light. {ECO:0000269|PubMed:12172798}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the lycopene beta-
CC cyclase family. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the phytoene/squalene
CC synthase family. {ECO:0000305}.
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DR EMBL; AJ426417; CAD19988.1; -; Genomic_DNA.
DR AlphaFoldDB; Q8X0Z1; -.
DR SMR; Q8X0Z1; -.
DR eggNOG; KOG1459; Eukaryota.
DR UniPathway; UPA00799; UER00773.
DR UniPathway; UPA00802; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0046905; F:15-cis-phytoene synthase activity; IMP:UniProtKB.
DR GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; IMP:UniProtKB.
DR GO; GO:0016872; F:intramolecular lyase activity; IEA:InterPro.
DR GO; GO:0045436; F:lycopene beta cyclase activity; IMP:UniProtKB.
DR GO; GO:0016120; P:carotene biosynthetic process; IMP:UniProtKB.
DR GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR CDD; cd00683; Trans_IPPS_HH; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR017825; Lycopene_cyclase_dom.
DR InterPro; IPR002060; Squ/phyt_synthse.
DR InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR InterPro; IPR044843; Trans_IPPS_bact-type.
DR InterPro; IPR033904; Trans_IPPS_HH.
DR Pfam; PF00494; SQS_PSY; 1.
DR SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
DR TIGRFAMs; TIGR03462; CarR_dom_SF; 2.
DR PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE 1: Evidence at protein level;
KW Carotenoid biosynthesis; Isomerase; Membrane; Multifunctional enzyme;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..612
FT /note="Bifunctional lycopene cyclase/phytoene synthase"
FT /id="PRO_0000409236"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..268
FT /note="Lycopene beta-cyclase"
FT /evidence="ECO:0000305|PubMed:12172798"
FT REGION 275..612
FT /note="Phytoene synthase"
FT /evidence="ECO:0000305|PubMed:12172798"
SQ SEQUENCE 612 AA; 69458 MW; 14F7D731B993AB65 CRC64;
MGWEYAQVHL KYTIPFGVVL AAVYRPLMSR LDVFKLVFLI TVSFFWVVKG LEANSCRLLL
FLPCMLILRG VLLRLTNADS PWDSYLIKNR IWTYPPGVVV GLTAWDIPAE ELFFFVIQTF
NTSLLYMILS KPTFHPIYLS KKTGWGKIAG QILFASAIIF GLVSVSSGGE GMYMGLILIW
ACPFLLFLWS ISYQFIVNLP WTNTALPIAL PTLYLWVVDT FALRRGTWSI TSGTKYGVVL
WDGLEIEEAV FFLLTNTLIV FGLIACDNNL AILDTFPEHF PRTKGVPSLL TIIRTLILPK
EKYDEERIQG LVSAVALLRK KSRSFYLASG TFEGRLRIDL IRLYAFCRAA DDLVDEAPSV
DDSRASIEKL RKFLDLAYEE NQEEPSQRLR EYVTSSIPEM FHMALLQLPT YYLPKQPLDD
LLKGFDTDLL FDRKSGAFPI ETTEDLDIYG SRVAGTVAEL CNHLILYHTP EAVPEDIQRE
VVASGQEMGI ALQYVNIARD IKTDAEIDRV YLPLSWLKEA QLTPEDVIQQ PHGPTIEALR
HKLLDRAFEK YNMAKGAIDK LPSEGKGPIR VAVESYMEIG RVLREKGPTM KKGRATVPKM
RRIRVAWSAL NK
