LCPS_MUCCL
ID LCPS_MUCCL Reviewed; 614 AA.
AC Q9UUQ6;
DT 19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 87.
DE RecName: Full=Bifunctional lycopene cyclase/phytoene synthase {ECO:0000303|PubMed:10951210};
DE Includes:
DE RecName: Full=Lycopene beta-cyclase {ECO:0000303|PubMed:10951210};
DE EC=5.5.1.19 {ECO:0000269|PubMed:10951210};
DE Includes:
DE RecName: Full=Phytoene synthase {ECO:0000303|PubMed:10951210};
DE EC=2.5.1.32 {ECO:0000269|PubMed:10951210};
GN Name=CARRP {ECO:0000303|PubMed:10951210};
OS Mucor circinelloides f. lusitanicus (Mucor racemosus var. lusitanicus).
OC Eukaryota; Fungi; Fungi incertae sedis; Mucoromycota; Mucoromycotina;
OC Mucoromycetes; Mucorales; Mucorineae; Mucoraceae; Mucor.
OX NCBI_TaxID=29924;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, INDUCTION,
RP AND VARIANTS LYS-78; ILE-86 AND GLY-409.
RC STRAIN=ATCC 1216b / BCRC 32522 / CBS 277.49 / NRRL 3631, MS21, MS35, and
RC MS36;
RX PubMed=10951210; DOI=10.1046/j.1432-1327.2000.01612.x;
RA Velayos A., Eslava A.P., Iturriaga E.A.;
RT "A bifunctional enzyme with lycopene cyclase and phytoene synthase
RT activities is encoded by the carRP gene of Mucor circinelloides.";
RL Eur. J. Biochem. 267:5509-5519(2000).
CC -!- FUNCTION: Bifunctional enzyme that catalyzes the reactions from
CC prephytoene diphosphate to phytoene and lycopene to beta-carotene via
CC the intermediate gamma-carotene. {ECO:0000269|PubMed:10951210}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=all-trans-lycopene = gamma-carotene; Xref=Rhea:RHEA:32219,
CC ChEBI:CHEBI:15948, ChEBI:CHEBI:27740; EC=5.5.1.19;
CC Evidence={ECO:0000269|PubMed:10951210};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=gamma-carotene = all-trans-beta-carotene;
CC Xref=Rhea:RHEA:32239, ChEBI:CHEBI:17579, ChEBI:CHEBI:27740;
CC EC=5.5.1.19; Evidence={ECO:0000269|PubMed:10951210};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2
CC diphosphate; Xref=Rhea:RHEA:34475, ChEBI:CHEBI:27787,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:58756; EC=2.5.1.32;
CC Evidence={ECO:0000269|PubMed:10951210};
CC -!- PATHWAY: Carotenoid biosynthesis; beta-carotene biosynthesis.
CC {ECO:0000269|PubMed:10951210}.
CC -!- PATHWAY: Carotenoid biosynthesis; phytoene biosynthesis; all-trans-
CC phytoene from geranylgeranyl diphosphate: step 1/1.
CC {ECO:0000269|PubMed:10951210}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: By blue light. {ECO:0000269|PubMed:10951210}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the lycopene beta-
CC cyclase family. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the phytoene/squalene
CC synthase family. {ECO:0000305}.
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DR EMBL; AJ250827; CAB60272.1; -; Genomic_DNA.
DR AlphaFoldDB; Q9UUQ6; -.
DR SMR; Q9UUQ6; -.
DR UniPathway; UPA00799; UER00773.
DR UniPathway; UPA00802; -.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0046905; F:15-cis-phytoene synthase activity; IDA:UniProtKB.
DR GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0016872; F:intramolecular lyase activity; IEA:InterPro.
DR GO; GO:0045436; F:lycopene beta cyclase activity; IDA:UniProtKB.
DR GO; GO:0016117; P:carotenoid biosynthetic process; IDA:UniProtKB.
DR CDD; cd00683; Trans_IPPS_HH; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR017825; Lycopene_cyclase_dom.
DR InterPro; IPR002060; Squ/phyt_synthse.
DR InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR InterPro; IPR044843; Trans_IPPS_bact-type.
DR InterPro; IPR033904; Trans_IPPS_HH.
DR Pfam; PF00494; SQS_PSY; 1.
DR SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR SFLD; SFLDG01018; Squalene/Phytoene_Synthase_Lik; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
DR TIGRFAMs; TIGR03462; CarR_dom_SF; 2.
DR PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE 1: Evidence at protein level;
KW Carotenoid biosynthesis; Isomerase; Membrane; Multifunctional enzyme;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..614
FT /note="Bifunctional lycopene cyclase/phytoene synthase"
FT /id="PRO_0000067441"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 120..140
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..239
FT /note="Lycopene beta-cyclase"
FT /evidence="ECO:0000305|PubMed:10951210"
FT REGION 246..614
FT /note="Phytoene synthase"
FT /evidence="ECO:0000305|PubMed:10951210"
FT VARIANT 78
FT /note="E -> K (in strain: MS21; loss of lycopene cyclase
FT activity)"
FT /evidence="ECO:0000269|PubMed:10951210"
FT VARIANT 86
FT /note="T -> I (in strain: MS35; loss of lycopene cyclase
FT activity)"
FT /evidence="ECO:0000269|PubMed:10951210"
FT VARIANT 409
FT /note="D -> G (in strain: MS36; loss of phytoene cyclase
FT activity)"
FT /evidence="ECO:0000269|PubMed:10951210"
SQ SEQUENCE 614 AA; 69841 MW; 599E6BB541087FE5 CRC64;
MLLTYMEVHL YYTLPVLGVL SWLSRPYYTA TDALKFKFLT LVAFTTASAW DNYIVYHKAW
SYCPTCVTAV IGYVPLEEYM FFIIMTLLTV AFTNLVMRWH LHSFFIRPET PVMQSVLVRL
VPITALLITA YKAWHLAVPG KPLFYGSCIL WYACPVLALL WFGAGEYMMR RPLAVLVSIA
LPTLFLCWVD VVAIGAGTWD ISLATSTGKF VVPHLPVEEF MFFALINTVL VFGTCAIDRT
MAILHLFKNK SPYQRPYQHS KSFLHQILEM TWAFCLPDQV LHSDTFHDLS VSWDILRKAS
KSFYTASAVF PGDVRQELGV LYAFCRATDD LCDNEQVPVQ TRKEQLILTH QFVSDLFGQK
TSAPTAIDWD FYNDQLPASC ISAFKSFTRL RHVLEAGAIK ELLDGYKWDL ERRSIRDQED
LRYYSACVAS SVGEMCTRII LAHADKPASR QQTQWIIQRA REMGLVLQYT NIARDIVTDS
EELGRCYLPQ DWLTEKEVAL IQGGLAREIG EERLLSLSHR LIYQADELMV VANKGIDKLP
SHCQGGVRAA CNVYASIGTK LKSYKHHYPS RAHVGNSKRV EIALLSVYNL YTAPIATSST
THCRQGKMRN LNTI
