LCRF_YERPE
ID LCRF_YERPE Reviewed; 271 AA.
AC P28808;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-1992, sequence version 1.
DT 25-MAY-2022, entry version 154.
DE RecName: Full=Thermoregulatory protein LcrF;
GN Name=lcrF; Synonyms=virF;
GN OrderedLocusNames=YPCD1.49, y5029, y0032, YP_pCD34;
OS Yersinia pestis.
OG Plasmid pCD1.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=632;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1624422; DOI=10.1128/jb.174.13.4275-4286.1992;
RA Goguen J.D., Minion C., Hoe N.P.;
RT "Temperature sensing in Yersinia pestis: regulation of yopE transcription
RT by lcrF.";
RL J. Bacteriol. 174:4275-4286(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=KIM5 / Biovar Mediaevalis;
RX PubMed=9746557; DOI=10.1128/iai.66.10.4611-4623.1998;
RA Perry R.D., Straley S.C., Fetherston J.D., Rose D.J., Gregor J.,
RA Blattner F.R.;
RT "DNA sequencing and analysis of the low-Ca2+-response plasmid pCD1 of
RT Yersinia pestis KIM5.";
RL Infect. Immun. 66:4611-4623(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=KIM5 / Biovar Mediaevalis;
RX PubMed=9748454; DOI=10.1128/jb.180.19.5192-5202.1998;
RA Hu P., Elliott J., McCready P., Skowronski E., Garnes J., Kobayashi A.,
RA Brubaker R.R., Garcia E.;
RT "Structural organization of virulence-associated plasmids of Yersinia
RT pestis.";
RL J. Bacteriol. 180:5192-5202(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CO-92 / Biovar Orientalis;
RX PubMed=11586360; DOI=10.1038/35097083;
RA Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G., Feltwell T.,
RA Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S.,
RA Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J.,
RA Stevens K., Whitehead S., Barrell B.G.;
RT "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL Nature 413:523-527(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=91001 / Biovar Mediaevalis;
RX PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L.,
RA Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H.,
RA Wang J., Huang P., Yang R.;
RT "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT avirulent to humans.";
RL DNA Res. 11:179-197(2004).
CC -!- FUNCTION: Transcriptional activator of the thermally regulated virulent
CC yopE gene. LcrF activity could be modulated by the interaction with an
CC inducer molecule serving as a temperature messenger. The availability
CC of the messenger would in turn be controlled by a temperature-
CC responsive process serving as a cellular thermometer.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M86690; AAA25558.1; -; Genomic_DNA.
DR EMBL; AF074612; AAC69782.1; -; Genomic_DNA.
DR EMBL; AF053946; AAC62554.1; -; Genomic_DNA.
DR EMBL; AL117189; CAB54926.1; -; Genomic_DNA.
DR EMBL; AE017043; AAS58553.1; -; Genomic_DNA.
DR PIR; A42884; A42884.
DR RefSeq; NP_395183.1; NC_003131.1.
DR RefSeq; NP_857733.1; NC_004836.1.
DR RefSeq; NP_857928.1; NC_004839.1.
DR RefSeq; WP_002212931.1; NZ_WUCM01000070.1.
DR AlphaFoldDB; P28808; -.
DR SMR; P28808; -.
DR IntAct; P28808; 4.
DR MINT; P28808; -.
DR STRING; 214092.5832469; -.
DR DNASU; 1149292; -.
DR EnsemblBacteria; AAS58553; AAS58553; YP_pCD34.
DR GeneID; 66841099; -.
DR KEGG; ype:YPCD1.49; -.
DR KEGG; ypm:YP_pCD34; -.
DR PATRIC; fig|1028802.3.peg.971; -.
DR eggNOG; COG2207; Bacteria.
DR HOGENOM; CLU_073843_2_0_6; -.
DR OMA; ISMEAGF; -.
DR Proteomes; UP000000815; Plasmid pCD1.
DR Proteomes; UP000001019; Plasmid pCD1.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:CACAO.
DR InterPro; IPR003313; AraC-bd.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR037923; HTH-like.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR018062; HTH_AraC-typ_CS.
DR InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR Pfam; PF02311; AraC_binding; 1.
DR Pfam; PF12833; HTH_18; 1.
DR PRINTS; PR00032; HTHARAC.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF51215; SSF51215; 1.
DR PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE 4: Predicted;
KW Activator; DNA-binding; Plasmid; Reference proteome; Transcription;
KW Transcription regulation; Virulence.
FT CHAIN 1..271
FT /note="Thermoregulatory protein LcrF"
FT /id="PRO_0000194529"
FT DOMAIN 167..265
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 184..205
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 232..255
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
SQ SEQUENCE 271 AA; 30833 MW; 4014863486DCE282 CRC64;
MASLEIIKLE WVTPIFKVVE HSQDGLYILL QGQISWQSSG QTYDLDEGNM LFLRRGSYAV
RCGTKEPCQL LWIPLPGSFL STFLHRFGSL LSEIGRDNST PKPLLIFNIS PILSQSIQNL
CAILERSDFP SVLTQLRIEE LLLLLAFSSQ GTLFLSALRH LGNRPEERLQ KFMEENYLQG
WKLSKFAREF GMGLTTFKEL FGTVYGISPR AWISERRILY AHQLLLNGKM SIVDIAMEAG
FSSQSYFTQS YRRRFGCTPS QARLTKIATT G
