ID   LCYB_NARPS              Reviewed;         503 AA.
AC   Q40424;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Lycopene beta cyclase, chloroplastic/chromoplastic;
DE            EC=5.5.1.19;
DE   Flags: Precursor;
GN   Name=LCY1; Synonyms=LYC;
OS   Narcissus pseudonarcissus (Daffodil).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Amaryllidaceae;
OC   Amaryllidoideae; Narcissus.
OX   NCBI_TaxID=39639;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Paracorolla;
RA   Al-Babili S., Hobeika E., Beyer P.;
RT   "A cDNA encoding lycopene cyclase from Narcissus pseudonarcissus L.";
RL   (er) Plant Gene Register PGR96-107(1996).
RN   [2]
RP   SUBCELLULAR LOCATION.
RX   PubMed=9288918; DOI=10.1111/j.1432-1033.1997.00942.x;
RA   Bonk M., Hoffmann B., von Lintig J., Schledz M., Al-Babili S., Hobeika E.,
RA   Kleinig H., Beyer P.;
RT   "Chloroplast import of four carotenoid biosynthetic enzymes in vitro
RT   reveals differential fates prior to membrane binding and oligomeric
RT   assembly.";
RL   Eur. J. Biochem. 247:942-950(1997).
CC   -!- FUNCTION: Catalyzes the double cyclization reaction which converts
CC       lycopene to beta-carotene and neurosporene to beta-zeacarotene.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a carotenoid psi-end group = a carotenoid beta-end derivative;
CC         Xref=Rhea:RHEA:55620, ChEBI:CHEBI:139114, ChEBI:CHEBI:139120;
CC         EC=5.5.1.19;
CC   -!- PATHWAY: Carotenoid biosynthesis; beta-carotene biosynthesis.
CC   -!- PATHWAY: Carotenoid biosynthesis; beta-zeacarotene biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC       {ECO:0000269|PubMed:9288918}. Plastid, chromoplast
CC       {ECO:0000269|PubMed:9288918}. Plastid, chromoplast membrane
CC       {ECO:0000269|PubMed:9288918}. Plastid, chloroplast membrane
CC       {ECO:0000269|PubMed:9288918}. Note=Exists as an inactive soluble form
CC       and an active membrane-bound form. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the lycopene cyclase family. {ECO:0000305}.
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DR   EMBL; X98796; CAA67331.1; -; mRNA.
DR   AlphaFoldDB; Q40424; -.
DR   SMR; Q40424; -.
DR   BioCyc; MetaCyc:MON-18248; -.
DR   UniPathway; UPA00802; -.
DR   UniPathway; UPA00805; -.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046862; C:chromoplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR010108; Lycopene_cyclase_b/e.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR01790; carotene-cycl; 1.
PE   2: Evidence at transcript level;
KW   Carotenoid biosynthesis; Chloroplast; Chromoplast; Isomerase; Membrane;
KW   NAD; Plastid; Transit peptide.
FT   TRANSIT         1..85
FT                   /note="Chloroplast and chromoplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           86..503
FT                   /note="Lycopene beta cyclase, chloroplastic/chromoplastic"
FT                   /id="PRO_0000018432"
FT   BINDING         90..117
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   503 AA;  56911 MW;  3FD1E355EF184D98 CRC64;
     MDTLLRTHNR LELLYPLHEL AKRHFLSPSP NPQNPNFKFF SRKPYQKKCR NGYIGVSSNQ
     LLDLVPEIKK EHLEFDLPLY DPSKALTLDL AVVGGGPLAR SCSTSLGGGL SVVSIDPNPK
     LIWPNNYGVW VDEFEDMDLL DCLDATWSGA IVYVDDRSTK NLSRPYARVN RKNLKSKMMK
     KCVSNGVRFH QATVVKAMHE EEKSYLICSD GVTIDARVVL DATGFSRCLV QYDKPYNPGY
     QVAYGILAEV EEHPFDVDKM VFMDWRDSHL NGKAELNERN AKIPTFLYAM PFSSNRIFLE
     ETSLVARPGL KMEDIQERMV ARLNHLGIRI KSIEEDERCV IPMGGPLPVI PQRVVGIGGT
     AGMVHPSTGY MVARTLAAAP IVANSIVQYL VSDSGLSGND LSADVWKDLW PIERRRQREF
     FCFGMDILLK LDLEGTRRFF DAFFDLEPRY WHGFLSSRLF LPELVPFGLS LFSHASNTCK
     LEIMAKGTLP LVNMINNLVQ DRD