ID   LCYB_ONCHC              Reviewed;         472 AA.
AC   D9IL23;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 1.
DT   03-AUG-2022, entry version 31.
DE   RecName: Full=Lycopene beta cyclase, chloroplastic;
DE            Short=OgLCY-B;
DE            EC=5.5.1.19;
DE   Flags: Precursor;
GN   Name=LCY-B;
OS   Oncidium hybrid cultivar (Orchid).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Orchidaceae;
OC   Epidendroideae; Cymbidieae; Oncidiinae; Oncidium.
OX   NCBI_TaxID=141207;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX   PubMed=20635095; DOI=10.1007/s00425-010-1222-x;
RA   Chiou C.Y., Pan H.A., Chuang Y.N., Yeh K.W.;
RT   "Differential expression of carotenoid-related genes determines diversified
RT   carotenoid coloration in floral tissues of Oncidium cultivars.";
RL   Planta 232:937-948(2010).
CC   -!- FUNCTION: Catalyzes the double cyclization reaction which converts
CC       lycopene to beta-carotene and neurosporene to beta-zeacarotene.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a carotenoid psi-end group = a carotenoid beta-end derivative;
CC         Xref=Rhea:RHEA:55620, ChEBI:CHEBI:139114, ChEBI:CHEBI:139120;
CC         EC=5.5.1.19;
CC   -!- PATHWAY: Carotenoid biosynthesis; beta-carotene biosynthesis.
CC   -!- PATHWAY: Carotenoid biosynthesis; beta-zeacarotene biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in flower buds and lips. Detected in
CC       roots and leaves. {ECO:0000269|PubMed:20635095}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during floral development.
CC       {ECO:0000269|PubMed:20635095}.
CC   -!- SIMILARITY: Belongs to the lycopene cyclase family. {ECO:0000305}.
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DR   EMBL; HM146076; ADJ67814.1; -; mRNA.
DR   AlphaFoldDB; D9IL23; -.
DR   SMR; D9IL23; -.
DR   UniPathway; UPA00802; -.
DR   UniPathway; UPA00805; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR010108; Lycopene_cyclase_b/e.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR01790; carotene-cycl; 1.
PE   2: Evidence at transcript level;
KW   Carotenoid biosynthesis; Chloroplast; Isomerase; NAD; Plastid;
KW   Transit peptide.
FT   TRANSIT         1..25
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..472
FT                   /note="Lycopene beta cyclase, chloroplastic"
FT                   /id="PRO_0000426710"
FT   REGION          1..27
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         62..90
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   472 AA;  52877 MW;  AEB64A368E2D7544 CRC64;
     MDALLTSPFI PLKKPSHNRK SNTTTASSSS SSFLQLIPQT KKETLDFDLP RYNTLISPTL
     DLAVVGGGPA GLAVAKRVSD AGLSVCSIDP YPNLVWPNNY GVWVDEFAAM DLLDCIDASW
     NSASVFIDDN NRKLLSRPYA RVDRRRLKSR LLNAYLSNGV RFHQSKVLNV VHEEAKSVLL
     CSDGTRIPAA VVLDATGFSR CLVQYQQPYN PGYQVAYGIL AEVEEHPFEL DQMVFMDWRD
     SHLGFRPELK KRNDGLPTFL YAMPFSSDLI FLEETSLVAR PGLLIEDIQE RMIERLRHLG
     IRIKRIEEDE RCVIPMGGPL PVLPQRVLGI GGTAGMVHPS TGYMVARTLA AAPIVADAIV
     GYIGGSGAEV SARVWKDLWP IERRRQREFF CFGMDVLLKL DLEGTRRFFD AFFGLEERYW
     HGFLSSRLFL PELVGFGFSL FGRASAGCRL EIMAKGTVPL IKMIRNLLKD LE