ID   LCYB_SOLLC              Reviewed;         500 AA.
AC   Q43503;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=Lycopene beta cyclase, chloroplastic;
DE            EC=5.5.1.19;
DE   Flags: Precursor;
GN   Name=LCY1; Synonyms=CRTL-1;
OS   Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC   Solanum subgen. Lycopersicon.
OX   NCBI_TaxID=4081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND CATALYTIC ACTIVITY.
RC   STRAIN=cv. VF36; TISSUE=Leaf;
RX   PubMed=8624411; DOI=10.1007/bf00019013;
RA   Pecker I., Gabbay R., Cunningham F.X. Jr., Hirschberg J.;
RT   "Cloning and characterization of the cDNA for lycopene beta-cyclase from
RT   tomato reveals decrease in its expression during fruit ripening.";
RL   Plant Mol. Biol. 30:807-819(1996).
CC   -!- FUNCTION: Catalyzes the double cyclization reaction which converts
CC       lycopene to beta-carotene and neurosporene to beta-zeacarotene.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a carotenoid psi-end group = a carotenoid beta-end derivative;
CC         Xref=Rhea:RHEA:55620, ChEBI:CHEBI:139114, ChEBI:CHEBI:139120;
CC         EC=5.5.1.19; Evidence={ECO:0000269|PubMed:8624411};
CC   -!- PATHWAY: Carotenoid biosynthesis; beta-carotene biosynthesis.
CC   -!- PATHWAY: Carotenoid biosynthesis; beta-zeacarotene biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the lycopene cyclase family. {ECO:0000305}.
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DR   EMBL; X86452; CAA60170.1; -; mRNA.
DR   PIR; S66350; S66350.
DR   PIR; S72505; S72505.
DR   RefSeq; NP_001234226.1; NM_001247297.2.
DR   RefSeq; NP_001303688.1; NM_001316759.1.
DR   AlphaFoldDB; Q43503; -.
DR   SMR; Q43503; -.
DR   STRING; 4081.Solyc04g040190.1.1; -.
DR   PaxDb; Q43503; -.
DR   PRIDE; Q43503; -.
DR   EnsemblPlants; Solyc04g040190.1.1; Solyc04g040190.1.1.1; Solyc04g040190.1.
DR   GeneID; 544104; -.
DR   Gramene; Solyc04g040190.1.1; Solyc04g040190.1.1.1; Solyc04g040190.1.
DR   KEGG; sly:544104; -.
DR   eggNOG; ENOG502QT2F; Eukaryota.
DR   HOGENOM; CLU_032956_1_0_1; -.
DR   InParanoid; Q43503; -.
DR   OMA; FVLMDFR; -.
DR   OrthoDB; 815606at2759; -.
DR   PhylomeDB; Q43503; -.
DR   BioCyc; MetaCyc:MON-12154; -.
DR   BRENDA; 5.5.1.19; 3101.
DR   UniPathway; UPA00802; -.
DR   UniPathway; UPA00805; -.
DR   Proteomes; UP000004994; Chromosome 4.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0045436; F:lycopene beta cyclase activity; IBA:GO_Central.
DR   GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0016120; P:carotene biosynthetic process; IBA:GO_Central.
DR   GO; GO:0006744; P:ubiquinone biosynthetic process; IBA:GO_Central.
DR   GO; GO:0016123; P:xanthophyll biosynthetic process; IBA:GO_Central.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR010108; Lycopene_cyclase_b/e.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR01790; carotene-cycl; 1.
PE   1: Evidence at protein level;
KW   Carotenoid biosynthesis; Chloroplast; Isomerase; NAD; Plastid;
KW   Reference proteome; Transit peptide.
FT   TRANSIT         1..81
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           82..500
FT                   /note="Lycopene beta cyclase, chloroplastic"
FT                   /id="PRO_0000018431"
FT   BINDING         86..114
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   500 AA;  56181 MW;  CF42F7D4684C04DD CRC64;
     MDTLLKTPNN LEFLNPHHGF AVKASTFRSE KHHNFGSRKF CETLGRSVCV KGSSSALLEL
     VPETKKENLD FELPMYDPSK GVVVDLAVVG GGPAGLAVAQ QVSEAGLSVC SIDPNPKLIW
     PNNYGVWVDE FEAMDLLDCL DATWSGAAVY IDDNTAKDLH RPYGRVNRKQ LKSKMMQKCI
     MNGVKFHQAK VIKVIHEESK SMLICNDGIT IQATVVLDAT GFSRSLVQYD KPYNPGYQVA
     YGILAEVEEH PFDVNKMVFM DWRDSHLKNN TDLKERNSRI PTFLYAMPFS SNRIFLEETS
     LVARPGLRID DIQERMVARL NHLGIKVKSI EEDEHCLIPM GGPLPVLPQR VVGIGGTAGM
     VHPSTGYMVA RTLAAAPVVA NAIIQYLGSE RSHSGNELST AVWKDLWPIE RRRQREFFCF
     GMDILLKLDL PATRRFFDAF FDLEPRYWHG FLSSRLFLPE LIVFGLSLFS HASNTSRFEI
     MTKGTVPLVN MINNLLQDKE