ID   LCYB_TOBAC              Reviewed;         500 AA.
AC   Q43578;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Lycopene beta cyclase, chloroplastic;
DE            EC=5.5.1.19;
DE   Flags: Precursor;
GN   Name=LCY1; Synonyms=CRTL-1;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND CATALYTIC ACTIVITY.
RC   STRAIN=cv. Samsun NN; TISSUE=Leaf;
RX   PubMed=8624411; DOI=10.1007/bf00019013;
RA   Pecker I., Gabbay R., Cunningham F.X. Jr., Hirschberg J.;
RT   "Cloning and characterization of the cDNA for lycopene beta-cyclase from
RT   tomato reveals decrease in its expression during fruit ripening.";
RL   Plant Mol. Biol. 30:807-819(1996).
CC   -!- FUNCTION: Catalyzes the double cyclization reaction which converts
CC       lycopene to beta-carotene and neurosporene to beta-zeacarotene.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a carotenoid psi-end group = a carotenoid beta-end derivative;
CC         Xref=Rhea:RHEA:55620, ChEBI:CHEBI:139114, ChEBI:CHEBI:139120;
CC         EC=5.5.1.19; Evidence={ECO:0000269|PubMed:8624411};
CC   -!- PATHWAY: Carotenoid biosynthesis; beta-carotene biosynthesis.
CC   -!- PATHWAY: Carotenoid biosynthesis; beta-zeacarotene biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the lycopene cyclase family. {ECO:0000305}.
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DR   EMBL; X81787; CAA57386.1; -; mRNA.
DR   PIR; S66349; S66349.
DR   PIR; S72506; S72506.
DR   RefSeq; NP_001311716.1; NM_001324787.1.
DR   AlphaFoldDB; Q43578; -.
DR   SMR; Q43578; -.
DR   STRING; 4097.Q43578; -.
DR   GeneID; 107763207; -.
DR   KEGG; ag:CAA57386; -.
DR   KEGG; nta:107763207; -.
DR   UniPathway; UPA00802; -.
DR   UniPathway; UPA00805; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0045436; F:lycopene beta cyclase activity; IBA:GO_Central.
DR   GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0016120; P:carotene biosynthetic process; IBA:GO_Central.
DR   GO; GO:0006744; P:ubiquinone biosynthetic process; IBA:GO_Central.
DR   GO; GO:0016123; P:xanthophyll biosynthetic process; IBA:GO_Central.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR010108; Lycopene_cyclase_b/e.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR01790; carotene-cycl; 1.
PE   1: Evidence at protein level;
KW   Carotenoid biosynthesis; Chloroplast; Isomerase; NAD; Plastid;
KW   Reference proteome; Transit peptide.
FT   TRANSIT         1..81
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           82..500
FT                   /note="Lycopene beta cyclase, chloroplastic"
FT                   /id="PRO_0000018433"
FT   BINDING         86..114
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   500 AA;  56067 MW;  2E3721B87EE6CBC8 CRC64;
     MDTLLKTPNK LEFLHPVHGF SVKASSFNSV KPHKFGSRKI CENWGKGVCV KAKSSALLEL
     VPETKKENLD FELPMYDPSK GLVVDLAVVG GGPAGLAVAQ QVSEAGLSVV SIDPSPKLIW
     PNNYGVWVDE FEAMDLLDCL DATWSGTVVY IDDNTTKDLD RPYGRVNRKQ LKSKMMQKCI
     LNGVKFHHAK VIKVIHEEAK SMLICNDGVT IQATVVLDAT GFSRCLVQYD KPYKPGYQVA
     YGILAEVEEH PFDTSKMVLM DWRDSHLGNN MELKERNRKV PTFLYAMPFS SNKIFLEETS
     LVARPGLRMD DIQERMVARL NHLGIKVKSI EEDEHCVIPM GGSLPVIPQR VVGTGGTAGL
     VHPSTGYMVA RTLAAAPVVA NAIIHYLGSE KDLLGNELSA AVWKDLWPIE RRRQREFFCF
     GMDILLKLDL PATRRFFDAF FDLEPRYWHG FLSSRLYLPE LIFFGLSLFS RASNTSRIEI
     MTKGTLPLVN MINNLLQDTE