ID   LCYD1_ORYSJ             Reviewed;         482 AA.
AC   Q9LGZ2; A0A0P0V1J9;
DT   09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Putative L-cysteine desulfhydrase 1;
DE            EC=4.4.1.28;
DE   AltName: Full=OsL-CDes1;
DE            Short=L-CDes1;
GN   OrderedLocusNames=Os01g0290100, LOC_Os01g18640; ORFNames=P0469E05.28;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447438; DOI=10.1038/nature01184;
RA   Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA   Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA   Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA   Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA   Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA   Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA   Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA   Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA   Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA   Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA   Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA   Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA   Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT   "The genome sequence and structure of rice chromosome 1.";
RL   Nature 420:312-316(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Catalyzes the production of hydrogen sulfide (H2S) from
CC       cysteine. {ECO:0000250|UniProtKB:Q9M1R1}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + L-cysteine = H(+) + hydrogen sulfide + NH4(+) +
CC         pyruvate; Xref=Rhea:RHEA:24931, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:28938, ChEBI:CHEBI:29919,
CC         ChEBI:CHEBI:35235; EC=4.4.1.28;
CC   -!- COFACTOR:
CC       Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC         Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the class-V pyridoxal-phosphate-dependent
CC       aminotransferase family. {ECO:0000305}.
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DR   EMBL; AP002480; BAA96579.1; -; Genomic_DNA.
DR   EMBL; AP008207; BAF04700.1; -; Genomic_DNA.
DR   EMBL; AP014957; BAS71653.1; -; Genomic_DNA.
DR   EMBL; AK069735; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_015613237.1; XM_015757751.1.
DR   AlphaFoldDB; Q9LGZ2; -.
DR   SMR; Q9LGZ2; -.
DR   STRING; 4530.OS01T0290100-01; -.
DR   PaxDb; Q9LGZ2; -.
DR   PRIDE; Q9LGZ2; -.
DR   EnsemblPlants; Os01t0290100-01; Os01t0290100-01; Os01g0290100.
DR   GeneID; 4325155; -.
DR   Gramene; Os01t0290100-01; Os01t0290100-01; Os01g0290100.
DR   KEGG; osa:4325155; -.
DR   eggNOG; KOG1549; Eukaryota.
DR   HOGENOM; CLU_003433_3_2_1; -.
DR   InParanoid; Q9LGZ2; -.
DR   OMA; TGNCHKW; -.
DR   OrthoDB; 516638at2759; -.
DR   PlantReactome; R-OSA-1119612; Cysteine degradation.
DR   Proteomes; UP000000763; Chromosome 1.
DR   Proteomes; UP000059680; Chromosome 1.
DR   Genevisible; Q9LGZ2; OS.
DR   GO; GO:0080146; F:L-cysteine desulfhydrase activity; IEA:RHEA.
DR   Gene3D; 3.40.640.10; -; 1.
DR   InterPro; IPR000192; Aminotrans_V_dom.
DR   InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR   InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR   Pfam; PF00266; Aminotran_5; 1.
DR   SUPFAM; SSF53383; SSF53383; 1.
PE   2: Evidence at transcript level;
KW   Lyase; Pyridoxal phosphate; Reference proteome.
FT   CHAIN           1..482
FT                   /note="Putative L-cysteine desulfhydrase 1"
FT                   /id="PRO_0000429505"
FT   REGION          1..45
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         276
FT                   /note="N6-(pyridoxal phosphate)lysine"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        287
FT                   /note="L -> W (in Ref. 5; AK069735)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        427
FT                   /note="A -> S (in Ref. 5; AK069735)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   482 AA;  52121 MW;  2F4DA048D5ABB7B1 CRC64;
     MASIPPDDDA AAAAAAGAAE NGYGNGKGNG NGPAPRPPPA KRPRSVISAA QIRAEFEHHE
     AGVARVNNGS FGCCPSSLLD AQARWQRLFI AQPDDFYFHA LQPGLRRSRA AVAGLVNAGD
     VAEVSLVDNA TTAAAIVLQH AAWSFAEGRF SRGDAVLMLH YAYGAVKKSI HAYVARAGAT
     VVEVPLPFPV ASADAIIAEF RAALDVAKAG GRKVRLAVID HITSMPSVVI PVKELVAICR
     EEGVDKVFID AAHSIGQVPV DVRDIGADFY TSNLHKWFFC PPAVAFLHTR KDDPIASQLH
     HPVVSHEYGN GLPMESGWIG TRDYSAQLVV PESIDFVNRF EGGIEGIRSR NHEKVIEMGK
     MLAEAWGTFL GTPPELCGSM VMVGLPGCLG VESDDDVMRM RTMLRKDFMV EVPIYYNSRR
     VEAQEMAKDK NGDAVTGYVR ISHQVYNVTE DYEKLRDAVN KLVADGFTSS KLRPSQKQET
     MA