ID   LCYE_ONCHC              Reviewed;         518 AA.
AC   D9IL24;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 1.
DT   03-AUG-2022, entry version 30.
DE   RecName: Full=Lycopene epsilon cyclase, chloroplastic {ECO:0000305};
DE            Short=OgLCY-E {ECO:0000303|PubMed:20635095};
DE            EC=5.5.1.18 {ECO:0000250|UniProtKB:Q38932};
DE   Flags: Precursor;
GN   Name=LCY-E {ECO:0000303|PubMed:20635095};
OS   Oncidium hybrid cultivar (Orchid).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Orchidaceae;
OC   Epidendroideae; Cymbidieae; Oncidiinae; Oncidium.
OX   NCBI_TaxID=141207;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX   PubMed=20635095; DOI=10.1007/s00425-010-1222-x;
RA   Chiou C.Y., Pan H.A., Chuang Y.N., Yeh K.W.;
RT   "Differential expression of carotenoid-related genes determines diversified
RT   carotenoid coloration in floral tissues of Oncidium cultivars.";
RL   Planta 232:937-948(2010).
CC   -!- FUNCTION: Catalyzes the single epsilon-cyclization reaction which
CC       converts lycopene to delta-carotene and neurosporene to alpha-
CC       zeacarotene. Required for lutein biosynthesis.
CC       {ECO:0000250|UniProtKB:Q38932}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a carotenoid psi-end group = a carotenoid epsilon-end group;
CC         Xref=Rhea:RHEA:55616, ChEBI:CHEBI:139114, ChEBI:CHEBI:139115;
CC         EC=5.5.1.18; Evidence={ECO:0000250|UniProtKB:Q38932};
CC   -!- PATHWAY: Carotenoid biosynthesis; alpha-zeacarotene biosynthesis.
CC       {ECO:0000305}.
CC   -!- PATHWAY: Carotenoid biosynthesis; delta-carotene biosynthesis.
CC       {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000255};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Expressed in leaves and roots. Detected in flower
CC       buds and lips. {ECO:0000269|PubMed:20635095}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during floral development.
CC       {ECO:0000269|PubMed:20635095}.
CC   -!- SIMILARITY: Belongs to the lycopene cyclase family. {ECO:0000305}.
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DR   EMBL; HM146077; ADJ67815.1; -; mRNA.
DR   AlphaFoldDB; D9IL24; -.
DR   SMR; D9IL24; -.
DR   UniPathway; UPA00801; -.
DR   UniPathway; UPA00804; -.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR010108; Lycopene_cyclase_b/e.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR01790; carotene-cycl; 1.
PE   2: Evidence at transcript level;
KW   Carotenoid biosynthesis; Chloroplast; Isomerase; Membrane; NAD; Plastid;
KW   Transit peptide; Transmembrane; Transmembrane helix.
FT   TRANSIT         1..?
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..518
FT                   /note="Lycopene epsilon cyclase, chloroplastic"
FT                   /id="PRO_0000426711"
FT   TRANSMEM        435..455
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        469..489
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         100..128
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   518 AA;  57744 MW;  ED8AED4A05DB5864 CRC64;
     MRSAAFSLPF CDWKCRRRLG AADKWPLLKR SFRILPPLIA GGATTEVKEV EKFADEEDFI
     KAGGSELFFV KMQERKPMEK QRKIADELTK ISSGDPMLDL IVIGCGPAGM SLAAEAGKRG
     LSVGLIGPDL PFTNNYGVWE DEFKGLGLES CIEHVWQDTI AYLDSSDPIL ISRAYGRVSR
     HLLHAELLRR CQETGVGFLD SKVEKIIEAS DGSSIVVCEG DLMLPCRLAT VASGAASGKL
     LQYEVGGPRV SVQTAYGVEA EVGNNPYDPR SMVFMDYRDH VKGKIISDEE YPTFLYVMPI
     SSTRVFYEET CLASRNAMPF DRLRSKLMSR LKAMGVSILK IYEEEWSYIP VGGSLPNTEQ
     KNLAFGVAAS MVHPATGYSI VRSLSEAPQY ASVITNILKR NSNSSQNNVI GSSYNPSVLA
     WRTLWPQEGK RQRAFFLFGL ALILQLDIDG IRIFFQTFFR LPDWMWQGFL GSTLSSAGLI
     WFAFYMFAIA PNSLRICLIK HLLSDPTGST MIREYLTL