ID   LCYE_SOLLC              Reviewed;         526 AA.
AC   O65837;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Lycopene epsilon cyclase, chloroplastic {ECO:0000305};
DE            EC=5.5.1.18 {ECO:0000250|UniProtKB:Q38932};
DE   Flags: Precursor;
GN   Name=CRTL-E-1 {ECO:0000312|EMBL:CAA74745.1};
OS   Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC   Solanum subgen. Lycopersicon.
OX   NCBI_TaxID=4081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. VF36; TISSUE=Leaf;
RA   Ronen G., Cohen M., Zamir D., Hirshberg J.;
RT   "Regulation of expression of the gene for lycopene epsilon cyclase during
RT   fruit ripening of tomato.";
RL   Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the single cyclization reaction which converts
CC       lycopene to delta-carotene and neurosporene to alpha-zeacarotene.
CC       Required for lutein biosynthesis. {ECO:0000250|UniProtKB:Q38932}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a carotenoid psi-end group = a carotenoid epsilon-end group;
CC         Xref=Rhea:RHEA:55616, ChEBI:CHEBI:139114, ChEBI:CHEBI:139115;
CC         EC=5.5.1.18; Evidence={ECO:0000250|UniProtKB:Q38932};
CC   -!- PATHWAY: Carotenoid biosynthesis; alpha-zeacarotene biosynthesis.
CC       {ECO:0000305}.
CC   -!- PATHWAY: Carotenoid biosynthesis; delta-carotene biosynthesis.
CC       {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000255};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the lycopene cyclase family. {ECO:0000305}.
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DR   EMBL; Y14387; CAA74745.1; -; mRNA.
DR   PIR; T07082; T07082.
DR   AlphaFoldDB; O65837; -.
DR   SMR; O65837; -.
DR   STRING; 4081.Solyc12g008980.1.1; -.
DR   PaxDb; O65837; -.
DR   PRIDE; O65837; -.
DR   eggNOG; ENOG502QT61; Eukaryota.
DR   InParanoid; O65837; -.
DR   BioCyc; MetaCyc:MON-12155; -.
DR   UniPathway; UPA00801; -.
DR   UniPathway; UPA00804; -.
DR   Proteomes; UP000004994; Unplaced.
DR   ExpressionAtlas; O65837; baseline and differential.
DR   GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   GO; GO:0016120; P:carotene biosynthetic process; IBA:GO_Central.
DR   GO; GO:0006744; P:ubiquinone biosynthetic process; IBA:GO_Central.
DR   GO; GO:0016123; P:xanthophyll biosynthetic process; IBA:GO_Central.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR010108; Lycopene_cyclase_b/e.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR01790; carotene-cycl; 1.
PE   2: Evidence at transcript level;
KW   Carotenoid biosynthesis; Chloroplast; Isomerase; Membrane; NAD; Plastid;
KW   Reference proteome; Transit peptide; Transmembrane; Transmembrane helix.
FT   TRANSIT         1..?
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..526
FT                   /note="Lycopene epsilon cyclase, chloroplastic"
FT                   /id="PRO_0000018435"
FT   TRANSMEM        443..463
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        477..497
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         108..136
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   526 AA;  58887 MW;  37357C3869DBDCAF CRC64;
     MECVGVQNVG AMAVLTRPRL NRWSGGELCQ EKSIFLAYEQ YESKCNSSSG SDSCVVDKED
     FADEEDYIKA GGSQLVFVQM QQKKDMDQQS KLSDELRQIS AGQTVLDLVV IGCGPAGLAL
     AAESAKLGLN VGLVGPDLPF TNNYGVWEDE FKDLGLQACI EHVWRDTIVY LDDDEPILIG
     RAYGRVSRHF LHEELLKRCV EAGVLYLNSK VDRIVEATNG QSLVECEGDV VIPCRFVTVA
     SGAASGKFLQ YELGSPRVSV QTAYGVEVEV DNNPFDPSLM VFMDYRDYLR HDAQSLEAKY
     PTFLYAMPMS PTRVFFEETC LASKDAMPFD LLKKKLMLRL NTLGVRIKEI YEEEWSYIPV
     GGSLPNTEQK TLAFGAAASM VHPATGYSVV RSLSEAPKCA SVLANILRQH YSKNMLTSSS
     IPSISTQAWN TLWPQERKRQ RSFFLFGLAL ILQLDIEGIR SFFRAFFRVP KWMWQGFLGS
     SLSSADLMLF AFYMFIIAPN DMRKGLIRHL LSDPTGATLI RTYLTF