ID   LDAH_CHICK              Reviewed;         324 AA.
AC   Q5F477;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 2.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Lipid droplet-associated hydrolase {ECO:0000250|UniProtKB:Q8BVA5};
DE            EC=3.1.1.- {ECO:0000250|UniProtKB:Q8BVA5};
DE   AltName: Full=Lipid droplet-associated serine hydrolase {ECO:0000250|UniProtKB:Q8BVA5};
GN   Name=LDAH {ECO:0000250|UniProtKB:Q8BVA5}; ORFNames=RCJMB04_2g19;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=CB; TISSUE=Bursa of Fabricius;
RX   PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA   Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P.,
RA   Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P.,
RA   Hayashizaki Y., Buerstedde J.-M.;
RT   "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT   function analysis.";
RL   Genome Biol. 6:R6.1-R6.9(2005).
CC   -!- FUNCTION: Probable serine lipid hydrolase associated with lipid
CC       droplets. Appears to lack cholesterol esterase activity. Appears to
CC       lack triglyceride lipase activity. {ECO:0000250|UniProtKB:Q8BVA5}.
CC   -!- SUBCELLULAR LOCATION: Lipid droplet {ECO:0000250|UniProtKB:Q8BVA5}.
CC       Endoplasmic reticulum {ECO:0000250|UniProtKB:Q8BVA5}. Note=Localizes to
CC       the endoplasmic reticulum in absence of lipid droplets and translocates
CC       to lipid droplets upon lipid storage induction.
CC       {ECO:0000250|UniProtKB:Q8BVA5}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. LDAH family.
CC       {ECO:0000305}.
CC   -!- CAUTION: The catalytic activity is unsure despite catalytic sites being
CC       conserved. {ECO:0000250|UniProtKB:Q8BVA5}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAH65057.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AJ851423; CAH65057.1; ALT_INIT; mRNA.
DR   RefSeq; NP_001026264.1; NM_001031093.1.
DR   AlphaFoldDB; Q5F477; -.
DR   STRING; 9031.ENSGALP00000030947; -.
DR   ESTHER; chick-cb043; LIDHydrolase.
DR   PaxDb; Q5F477; -.
DR   GeneID; 421967; -.
DR   KEGG; gga:421967; -.
DR   CTD; 60526; -.
DR   VEuPathDB; HostDB:geneid_421967; -.
DR   eggNOG; KOG3975; Eukaryota.
DR   InParanoid; Q5F477; -.
DR   OrthoDB; 1163030at2759; -.
DR   PhylomeDB; Q5F477; -.
DR   PRO; PR:Q5F477; -.
DR   Proteomes; UP000000539; Unplaced.
DR   GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR   GO; GO:0005811; C:lipid droplet; ISS:UniProtKB.
DR   GO; GO:0004771; F:sterol esterase activity; ISS:UniProtKB.
DR   GO; GO:0019915; P:lipid storage; IBA:GO_Central.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR019363; LDAH.
DR   PANTHER; PTHR13390; PTHR13390; 1.
DR   Pfam; PF10230; LIDHydrolase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Hydrolase; Lipid droplet; Reference proteome.
FT   CHAIN           1..324
FT                   /note="Lipid droplet-associated hydrolase"
FT                   /id="PRO_0000300128"
FT   ACT_SITE        136
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10037"
FT   ACT_SITE        270
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:Q9H6V9"
FT   ACT_SITE        299
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:Q9H6V9"
SQ   SEQUENCE   324 AA;  36882 MW;  F6679767588D445A CRC64;
     MRSVSEEPVP LHEEFIYCCG AATHVLKCGP WKDLSKDESK NLPRLLFMII PGNPGLAGYY
     RTFIKALYCG LNQQYPVWVV SHAGHCKPPS GMEMIEDTDI KELEDVFGLN GQVEHKLNFL
     KKNVSKDIKL VLIAHSIGCY ITLEMMKRAS ELQVLRSVLL FPTIERMAQS PQGKLMTPLL
     CKLRYALYMP VYLLSFLPEG VKASLVRFAL RGMKTCDESS ITTSVNLFSV DCIANILYMA
     SQEMMKVVER DSTTIKQNLK KLIFYYGTGD SWCPQNYYDE IKMDFPDGDI RLCEKGLRHA
     FVLDASKEMA AMITDWLRDD LTKL