ID   LDAH_DICDI              Reviewed;         304 AA.
AC   Q54LL8;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Lipid droplet-associated hydrolase {ECO:0000250|UniProtKB:Q8BVA5};
DE            EC=3.1.1.- {ECO:0000250|UniProtKB:Q8BVA5};
DE   AltName: Full=Lipid droplet-associated serine hydrolase {ECO:0000250|UniProtKB:Q8BVA5};
GN   ORFNames=DDB_G0286581;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Probable serine lipid hydrolase associated with lipid
CC       droplets. Appears to lack cholesterol esterase activity. Appears to
CC       lack triglyceride lipase activity. {ECO:0000250|UniProtKB:Q8BVA5}.
CC   -!- SUBCELLULAR LOCATION: Lipid droplet {ECO:0000250|UniProtKB:Q8BVA5}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. LDAH family.
CC       {ECO:0000305}.
CC   -!- CAUTION: The catalytic activity is unsure despite catalytic sites being
CC       conserved. {ECO:0000250|UniProtKB:Q8BVA5}.
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DR   EMBL; AAFI02000089; EAL64046.1; -; Genomic_DNA.
DR   RefSeq; XP_637543.1; XM_632451.1.
DR   AlphaFoldDB; Q54LL8; -.
DR   STRING; 44689.DDB0305142; -.
DR   ESTHER; dicdi-u554; LIDHydrolase.
DR   PaxDb; Q54LL8; -.
DR   EnsemblProtists; EAL64046; EAL64046; DDB_G0286581.
DR   GeneID; 8625682; -.
DR   KEGG; ddi:DDB_G0286581; -.
DR   dictyBase; DDB_G0286581; -.
DR   eggNOG; KOG3975; Eukaryota.
DR   HOGENOM; CLU_018394_2_1_1; -.
DR   InParanoid; Q54LL8; -.
DR   OMA; PVWVIGH; -.
DR   PhylomeDB; Q54LL8; -.
DR   PRO; PR:Q54LL8; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0005811; C:lipid droplet; IBA:GO_Central.
DR   GO; GO:0016298; F:lipase activity; IEA:InterPro.
DR   GO; GO:0019915; P:lipid storage; IBA:GO_Central.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR019363; LDAH.
DR   PANTHER; PTHR13390; PTHR13390; 1.
DR   Pfam; PF10230; LIDHydrolase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Lipid droplet; Reference proteome.
FT   CHAIN           1..304
FT                   /note="Lipid droplet-associated hydrolase"
FT                   /id="PRO_0000327947"
FT   ACT_SITE        119
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10037"
FT   ACT_SITE        250
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:Q9H6V9"
FT   ACT_SITE        279
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:Q9H6V9"
SQ   SEQUENCE   304 AA;  35128 MW;  1C5B40698ED3C7B0 CRC64;
     MELIEEIINL DEAIQGETRT EIIYTKSQTP SNIKIIVIAG NPGIESFYQE FVKVLNLSFN
     SKYDIYGVGH IGHCGKIENK TFSVEEQIKH KELFLEYLLK NKYGDKDRKD IKFILIGHSV
     GSYISLKVVS RFSEKFEFLS VVNLFPTFKN LYDGLSPFIK MVVMRESTRN GLSTFLHYIP
     SIVVSNVLKW ILPSDESRIA VQSKINYYSA LNILYMAYTE TEDIKEIDDE CHSVFNSRLN
     QLLFIYGQTD SYTPKSFYDE MKQLYPAGNI EYSSSYVPHA FVLHHSQEVA LRVSEWLSLN
     ILKN