ID   LDAH_RAT                Reviewed;         325 AA.
AC   Q5HZX7;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Lipid droplet-associated hydrolase {ECO:0000250|UniProtKB:Q8BVA5};
DE            EC=3.1.1.- {ECO:0000250|UniProtKB:Q8BVA5};
DE   AltName: Full=Lipid droplet-associated serine hydrolase {ECO:0000250|UniProtKB:Q8BVA5};
GN   Name=Ldah {ECO:0000250|UniProtKB:Q8BVA5};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Probable serine lipid hydrolase associated with lipid
CC       droplets. Appears to lack cholesterol esterase activity. Appears to
CC       lack triglyceride lipase activity. Highly expressed in macrophage-rich
CC       areas in atherosclerotic lesions, suggesting that it could promote
CC       cholesterol ester turnover in macrophages.
CC       {ECO:0000250|UniProtKB:Q8BVA5}.
CC   -!- SUBCELLULAR LOCATION: Lipid droplet {ECO:0000250|UniProtKB:Q8BVA5}.
CC       Endoplasmic reticulum {ECO:0000250|UniProtKB:Q8BVA5}. Note=Localizes to
CC       the endoplasmic reticulum in absence of lipid droplets and translocates
CC       to lipid droplets upon lipid storage induction.
CC       {ECO:0000250|UniProtKB:Q8BVA5}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. LDAH family.
CC       {ECO:0000305}.
CC   -!- CAUTION: The catalytic activity is unsure despite catalytic sites being
CC       conserved. {ECO:0000250|UniProtKB:Q8BVA5}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC088848; AAH88848.1; -; mRNA.
DR   RefSeq; NP_001014097.1; NM_001014075.1.
DR   RefSeq; XP_008762813.1; XM_008764591.2.
DR   AlphaFoldDB; Q5HZX7; -.
DR   STRING; 10116.ENSRNOP00000033708; -.
DR   ESTHER; rat-Ldah; LIDHydrolase.
DR   PhosphoSitePlus; Q5HZX7; -.
DR   PaxDb; Q5HZX7; -.
DR   Ensembl; ENSRNOT00000115305; ENSRNOP00000088267; ENSRNOG00000021475.
DR   GeneID; 313949; -.
DR   KEGG; rno:313949; -.
DR   CTD; 60526; -.
DR   RGD; 1311648; Ldah.
DR   eggNOG; KOG3975; Eukaryota.
DR   GeneTree; ENSGT00390000009688; -.
DR   HOGENOM; CLU_018394_2_1_1; -.
DR   InParanoid; Q5HZX7; -.
DR   OMA; PVWVIGH; -.
DR   OrthoDB; 1163030at2759; -.
DR   PhylomeDB; Q5HZX7; -.
DR   TreeFam; TF313050; -.
DR   PRO; PR:Q5HZX7; -.
DR   Proteomes; UP000002494; Chromosome 6.
DR   Bgee; ENSRNOG00000021475; Expressed in duodenum and 19 other tissues.
DR   Genevisible; Q5HZX7; RN.
DR   GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR   GO; GO:0005811; C:lipid droplet; ISS:UniProtKB.
DR   GO; GO:0016298; F:lipase activity; ISO:RGD.
DR   GO; GO:0004771; F:sterol esterase activity; ISS:UniProtKB.
DR   GO; GO:0019915; P:lipid storage; IBA:GO_Central.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR019363; LDAH.
DR   PANTHER; PTHR13390; PTHR13390; 1.
DR   Pfam; PF10230; LIDHydrolase; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   PROSITE; PS00120; LIPASE_SER; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Hydrolase; Lipid droplet; Reference proteome.
FT   CHAIN           1..325
FT                   /note="Lipid droplet-associated hydrolase"
FT                   /id="PRO_0000300127"
FT   ACT_SITE        139
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10037"
FT   ACT_SITE        271
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:Q9H6V9"
FT   ACT_SITE        300
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250|UniProtKB:Q9H6V9"
SQ   SEQUENCE   325 AA;  36881 MW;  C04DE2A5FBB6EB5F CRC64;
     MASEVEDGVP VHEEFFLCGG VETQIIKCGP WTNLFDKQGV SKPKHLIFVI PGNPGLSPFY
     VPFAKALYSL VKGHFPVWII SHAGFCLTPK DKKILTAPQE PNAQEIEDIY GLNGQIEHKI
     AFLRAHVPKD VKLIFIGHSV GSYIALHVMN RAPELPVVHT FLLFPTIERM SESPNGKFAT
     PFLCRFRYML YAASYLIFKP CPEMIKSFIV QKVLEKMSFK SELRLTDLLQ PFCLANAAYL
     GGQEMIHVVK RDDGIIKELL PKLTFYYGKT DGWCPVNYYE DMKRDFPEAN LHLCEKGIPH
     AFVLGFSQEM AAMVADWINN RLPKK