LDHB_FUNHE
ID LDHB_FUNHE Reviewed; 334 AA.
AC P20373;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 2.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=L-lactate dehydrogenase B chain;
DE Short=LDH-B;
DE EC=1.1.1.27 {ECO:0000250|UniProtKB:P07195};
GN Name=ldhb;
OS Fundulus heteroclitus (Killifish) (Mummichog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Atherinomorphae; Cyprinodontiformes; Fundulidae; Fundulus.
OX NCBI_TaxID=8078;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RX PubMed=2615640; DOI=10.1093/oxfordjournals.molbev.a040559;
RA Crawford D.L., Constantino H.R., Powers D.A.;
RT "Lactate dehydrogenase-B cDNA from the teleost Fundulus heteroclitus:
RT evolutionary implications.";
RL Mol. Biol. Evol. 6:369-383(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8105474; DOI=10.1073/pnas.90.20.9271;
RA Bernardi G., Sordino P., Powers D.A.;
RT "Concordant mitochondrial and nuclear DNA phylogenies for populations of
RT the teleost fish Fundulus heteroclitus.";
RL Proc. Natl. Acad. Sci. U.S.A. 90:9271-9274(1993).
CC -!- FUNCTION: Interconverts simultaneously and stereospecifically pyruvate
CC and lactate with concomitant interconversion of NADH and NAD(+).
CC {ECO:0000250|UniProtKB:P07195}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-lactate + NAD(+) = H(+) + NADH + pyruvate;
CC Xref=Rhea:RHEA:23444, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16651, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.27;
CC Evidence={ECO:0000250|UniProtKB:P07195};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:23445;
CC Evidence={ECO:0000250|UniProtKB:P07195};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:23446;
CC Evidence={ECO:0000250|UniProtKB:P07195};
CC -!- PATHWAY: Fermentation; pyruvate fermentation to lactate; (S)-lactate
CC from pyruvate: step 1/1. {ECO:0000250|UniProtKB:P07195}.
CC -!- SUBUNIT: Homotetramer.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- POLYMORPHISM: Isolates from various regions of the US and Canada show
CC some variations. PubMed:8105474 has sequenced LDH-B isolates called
CC Florida 1A, 1B, 2A and 2B; Georgia 2A and 2B; Maine 2A; New Jersey
CC 121A, 121B, 137A, 137B and 197B; Nova Scotia 1A, 1B, 2A and 2B.
CC -!- SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family.
CC {ECO:0000305}.
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DR EMBL; L23792; AAA49298.1; -; Genomic_DNA.
DR EMBL; M33969; AAA49306.1; -; mRNA.
DR EMBL; L23781; AAA49291.1; -; Genomic_DNA.
DR EMBL; L23782; AAA49289.1; -; Genomic_DNA.
DR EMBL; L23783; AAA49292.1; -; Genomic_DNA.
DR EMBL; L23784; AAA49288.1; -; Genomic_DNA.
DR EMBL; L23785; AAA49304.1; -; Genomic_DNA.
DR EMBL; L23786; AAA49305.1; -; Genomic_DNA.
DR EMBL; L23787; AAA49293.1; -; Genomic_DNA.
DR EMBL; L23788; AAA49294.1; -; Genomic_DNA.
DR EMBL; L23789; AAA49295.1; -; Genomic_DNA.
DR EMBL; L23790; AAA49296.1; -; Genomic_DNA.
DR EMBL; L23791; AAA49297.1; -; Genomic_DNA.
DR EMBL; L23793; AAA49299.1; -; Genomic_DNA.
DR EMBL; L23794; AAA49300.1; -; Genomic_DNA.
DR EMBL; L23795; AAA49301.1; -; Genomic_DNA.
DR EMBL; L23796; AAA49302.1; -; Genomic_DNA.
DR EMBL; L23797; AAA49303.1; -; Genomic_DNA.
DR PIR; A32430; A32430.
DR PIR; I50555; I50555.
DR RefSeq; NP_001296887.1; NM_001309958.1.
DR AlphaFoldDB; P20373; -.
DR SMR; P20373; -.
DR STRING; 8078.ENSFHEP00000012921; -.
DR GeneID; 105929819; -.
DR CTD; 30497; -.
DR OrthoDB; 1204514at2759; -.
DR UniPathway; UPA00554; UER00611.
DR Proteomes; UP000265000; Whole Genome Shotgun Assembly.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004459; F:L-lactate dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR Gene3D; 3.90.110.10; -; 1.
DR HAMAP; MF_00488; Lactate_dehydrog; 1.
DR InterPro; IPR001557; L-lactate/malate_DH.
DR InterPro; IPR011304; L-lactate_DH.
DR InterPro; IPR018177; L-lactate_DH_AS.
DR InterPro; IPR022383; Lactate/malate_DH_C.
DR InterPro; IPR001236; Lactate/malate_DH_N.
DR InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF02866; Ldh_1_C; 1.
DR Pfam; PF00056; Ldh_1_N; 1.
DR PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR PRINTS; PR00086; LLDHDRGNASE.
DR SUPFAM; SSF51735; SSF51735; 1.
DR SUPFAM; SSF56327; SSF56327; 1.
DR TIGRFAMs; TIGR01771; L-LDH-NAD; 1.
DR PROSITE; PS00064; L_LDH; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; NAD; Oxidoreductase.
FT CHAIN 1..334
FT /note="L-lactate dehydrogenase B chain"
FT /id="PRO_0000168476"
FT ACT_SITE 194
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 30..58
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 100
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 107
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 139
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 139
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 170
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 249
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT VARIANT 10
FT /note="T -> P (in strain: Isolate New Jersey 121A)"
FT VARIANT 45
FT /note="L -> P (in strain: Isolate Georgia 2B)"
FT VARIANT 49
FT /note="L -> P (in strain: Isolate Nova Scotia 2B)"
FT VARIANT 112
FT /note="Q -> R (in strain: Isolate New Jersey 137A)"
FT VARIANT 114
FT /note="N -> S (in strain: Isolate New Jersey 137A)"
FT VARIANT 185
FT /note="A -> S (in strain: Isolate Maine 2A, Isolate New
FT Jersey 121B, Isolate New Jersey 197B, Isolate Nova Scotia
FT 1A, Isolate Nova Scotia 1B, Isolate Nova Scotia 2A and
FT Isolate Nova Scotia 2B)"
FT VARIANT 287
FT /note="E -> D (in strain: Isolate Nova Scotia 1B and
FT Isolate Nova Scotia 2B)"
FT VARIANT 301
FT /note="V -> L (in strain: Isolate New Jersey 197B)"
FT VARIANT 311
FT /note="D -> A (in strain: Isolate Maine 2A, Isolate New
FT Jersey 137A, Isolate New Jersey 137B, Isolate New Jersey
FT 197B, Isolate Nova Scotia 1A, Isolate Nova Scotia 1B,
FT Isolate Nova Scotia 2A and Isolate Nova Scotia 2B)"
SQ SEQUENCE 334 AA; 36224 MW; CE8CEF79D7C07B97 CRC64;
MSSVLQKLIT PLASSSAEPP RNKVTVVGVG QVGMACAVSI LLRDLCDELA LVDVMEDRLK
GEMMDLQHGL LFLKTSKVVA DKDYAVTANS RLVVVTAGVR QQEGESRLNL VQRNVNVFKC
IIPQIIKYSP NCTILVVSNP VDVLTYVTWK LSGLPKHRVI GSGTNLDSAR FRYMMAERLG
IHASAFNGWV LGEHGDTSVP VWSGANVAGV SLQKLNPEIG TDGDKEQWKA THKAVVDSAY
EVIKLKGYTN WAIGFSVADL TESIVKNLSR VHPVSTMVKD MFGIGEEVFL SLPCVLNGSG
VGSVVNMTLT DAEVAQLKKS ADTLWGIQKD LKDL
