ID   LDH_CUPNH               Reviewed;         349 AA.
AC   Q07251; Q0KDV7;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-MAY-2022, entry version 114.
DE   RecName: Full=L-lactate dehydrogenase;
DE            EC=1.1.1.27;
GN   Name=ldh; OrderedLocusNames=H16_A0666;
OS   Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442
OS   / H16 / Stanier 337) (Ralstonia eutropha).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=381666;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-27.
RX   PubMed=8405966; DOI=10.1111/j.1574-6968.1993.tb06453.x;
RA   Jendrossek D., Kratzin H.D., Steinbuechel A.;
RT   "The Alcaligenes eutrophus ldh structural gene encodes a novel type of
RT   lactate dehydrogenase.";
RL   FEMS Microbiol. Lett. 112:229-235(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337;
RX   PubMed=16964242; DOI=10.1038/nbt1244;
RA   Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R.,
RA   Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I.,
RA   Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.;
RT   "Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia
RT   eutropha H16.";
RL   Nat. Biotechnol. 24:1257-1262(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-lactate + NAD(+) = H(+) + NADH + pyruvate;
CC         Xref=Rhea:RHEA:23444, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16651, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.27;
CC   -!- PATHWAY: Fermentation; pyruvate fermentation to lactate; (S)-lactate
CC       from pyruvate: step 1/1.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the LDH2/MDH2 oxidoreductase family.
CC       {ECO:0000305}.
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DR   EMBL; Z22737; CAA80432.1; -; Genomic_DNA.
DR   EMBL; AM260479; CAJ91814.1; -; Genomic_DNA.
DR   PIR; I39535; I39535.
DR   RefSeq; WP_011614641.1; NZ_CP039287.1.
DR   AlphaFoldDB; Q07251; -.
DR   SMR; Q07251; -.
DR   STRING; 381666.H16_A0666; -.
DR   EnsemblBacteria; CAJ91814; CAJ91814; H16_A0666.
DR   GeneID; 57642762; -.
DR   KEGG; reh:H16_A0666; -.
DR   PATRIC; fig|381666.6.peg.1034; -.
DR   eggNOG; COG2055; Bacteria.
DR   HOGENOM; CLU_040452_1_0_4; -.
DR   OMA; MAHIYLD; -.
DR   OrthoDB; 1374098at2; -.
DR   UniPathway; UPA00554; UER00611.
DR   Proteomes; UP000008210; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004459; F:L-lactate dehydrogenase activity; IEA:UniProtKB-EC.
DR   Gene3D; 1.10.1530.10; -; 1.
DR   Gene3D; 3.30.1370.60; -; 1.
DR   InterPro; IPR043144; Mal/L-sulf/L-lact_DH-like_ah.
DR   InterPro; IPR043143; Mal/L-sulf/L-lact_DH-like_NADP.
DR   InterPro; IPR036111; Mal/L-sulfo/L-lacto_DH-like_sf.
DR   InterPro; IPR003767; Malate/L-lactate_DH-like.
DR   PANTHER; PTHR11091; PTHR11091; 1.
DR   Pfam; PF02615; Ldh_2; 1.
DR   SUPFAM; SSF89733; SSF89733; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase;
KW   Reference proteome.
FT   CHAIN           1..349
FT                   /note="L-lactate dehydrogenase"
FT                   /id="PRO_0000083826"
FT   REGION          199..219
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   349 AA;  36756 MW;  7A8FB0E7859CBC04 CRC64;
     MKISLTSARQ LARDILAAQQ VPADIADDVA EHLVESDRCG YISHGLSILP NYRTALDGHS
     VNPQGRAKCV LDQGTLMVFD GDGGFGQHVG KSVMQAAIER VRQHGHCIVT LRRSHHLGRM
     GHYGEMAAAA GFVLLSFTNV INRAPVVAPF GGRVARLTTN PLCFAGPMPN GRPPLVVDIA
     TSAIAINKAR VLAEKGEPAP EGSIIGADGN PTTDASTMFG EHPGALLPFG GHKGYALGVV
     AELLAGVLSG GGTIQPDNPR GGVATNNLFA VLLNPALDLG LDWQSAEVEA FVRYLHDTPP
     APGVDRVQYP GEYEAANRAQ ASDTLNINPA IWRNLERLAQ SLNVAVPTA