LDH_DROME
ID LDH_DROME Reviewed; 332 AA.
AC Q95028; Q9VRW6;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=L-lactate dehydrogenase {ECO:0000312|FlyBase:FBgn0001258};
DE EC=1.1.1.27;
GN Name=Ldh {ECO:0000312|FlyBase:FBgn0001258};
GN Synonyms=Imp-L3 {ECO:0000303|PubMed:9094207},
GN ImpL3 {ECO:0000312|FlyBase:FBgn0001258};
GN ORFNames=CG10160 {ECO:0000312|FlyBase:FBgn0001258};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Oregon-R;
RX PubMed=9094207;
RX DOI=10.1002/(sici)1520-6408(1997)20:1<11::aid-dvg2>3.0.co;2-c;
RA Abu-Shumays R.L., Fristrom J.W.;
RT "IMP-L3, A 20-hydroxyecdysone-responsive gene encodes Drosophila lactate
RT dehydrogenase: structural characterization and developmental studies.";
RL Dev. Genet. 20:11-22(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-lactate + NAD(+) = H(+) + NADH + pyruvate;
CC Xref=Rhea:RHEA:23444, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16651, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.27;
CC -!- PATHWAY: Fermentation; pyruvate fermentation to lactate; (S)-lactate
CC from pyruvate: step 1/1.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family.
CC {ECO:0000305}.
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DR EMBL; U68038; AAB07594.1; -; mRNA.
DR EMBL; AE014296; AAF50666.1; -; Genomic_DNA.
DR RefSeq; NP_001261474.1; NM_001274545.2.
DR RefSeq; NP_001261475.1; NM_001274546.1.
DR RefSeq; NP_001261476.1; NM_001274547.1.
DR RefSeq; NP_476581.1; NM_057233.5.
DR AlphaFoldDB; Q95028; -.
DR SMR; Q95028; -.
DR BioGRID; 69949; 65.
DR DIP; DIP-17530N; -.
DR IntAct; Q95028; 3.
DR STRING; 7227.FBpp0304375; -.
DR PaxDb; Q95028; -.
DR PRIDE; Q95028; -.
DR DNASU; 45880; -.
DR EnsemblMetazoa; FBtr0077008; FBpp0076716; FBgn0001258.
DR EnsemblMetazoa; FBtr0332065; FBpp0304375; FBgn0001258.
DR EnsemblMetazoa; FBtr0332066; FBpp0304376; FBgn0001258.
DR EnsemblMetazoa; FBtr0332067; FBpp0304377; FBgn0001258.
DR GeneID; 45880; -.
DR KEGG; dme:Dmel_CG10160; -.
DR CTD; 45880; -.
DR FlyBase; FBgn0001258; Ldh.
DR VEuPathDB; VectorBase:FBgn0001258; -.
DR eggNOG; KOG1495; Eukaryota.
DR GeneTree; ENSGT00940000164122; -.
DR HOGENOM; CLU_045401_0_2_1; -.
DR InParanoid; Q95028; -.
DR OMA; ASCAEYI; -.
DR OrthoDB; 1204514at2759; -.
DR PhylomeDB; Q95028; -.
DR Reactome; R-DME-70268; Pyruvate metabolism.
DR SignaLink; Q95028; -.
DR UniPathway; UPA00554; UER00611.
DR BioGRID-ORCS; 45880; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 45880; -.
DR PRO; PR:Q95028; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0001258; Expressed in seminal fluid secreting gland and 30 other tissues.
DR ExpressionAtlas; Q95028; baseline and differential.
DR Genevisible; Q95028; DM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004459; F:L-lactate dehydrogenase activity; IBA:GO_Central.
DR GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR Gene3D; 3.90.110.10; -; 1.
DR HAMAP; MF_00488; Lactate_dehydrog; 1.
DR InterPro; IPR001557; L-lactate/malate_DH.
DR InterPro; IPR011304; L-lactate_DH.
DR InterPro; IPR018177; L-lactate_DH_AS.
DR InterPro; IPR022383; Lactate/malate_DH_C.
DR InterPro; IPR001236; Lactate/malate_DH_N.
DR InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF02866; Ldh_1_C; 1.
DR Pfam; PF00056; Ldh_1_N; 1.
DR PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR PRINTS; PR00086; LLDHDRGNASE.
DR SUPFAM; SSF51735; SSF51735; 1.
DR SUPFAM; SSF56327; SSF56327; 1.
DR TIGRFAMs; TIGR01771; L-LDH-NAD; 1.
DR PROSITE; PS00064; L_LDH; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; NAD; Oxidoreductase; Reference proteome.
FT CHAIN 1..332
FT /note="L-lactate dehydrogenase"
FT /id="PRO_0000168494"
FT ACT_SITE 193
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 29..57
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 99
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 106
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 138
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 138
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 169
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 248
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 332 AA; 35537 MW; E49F1EE2399440F1 CRC64;
MAAIKDSLLA QVAEVLPSSG HKVTIVGIGQ VGMASAFSIL AQNVSKEVCL IDVCADKLQG
ELMDLQHGSN FLKNPQITAS TDFAASANSR LCIVTAGVRQ KEGESRLSLV QRNTDILKNI
IPKLVEYSPD TILLMVSNPV DIMTYVAWKL SGLPKNRVIG SGTNLDSSRF RFLMSQRLGV
APTSCHGWII GEHGDSSVPV WSGVNIAGVR LRELNPILGT GEDPEKWNEL HKQVVDSAYE
VIKLKGYTSW AIGLSTASLA SAILRNTSSV AAVSTSVLGE HGIDKDVFLS LPCVLNANGV
TSVVKQILTP TEVEQLQKSA NIMSDVQAGL KF
