LDH_SELRU
ID LDH_SELRU Reviewed; 318 AA.
AC Q9EVR0;
DT 21-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=L-lactate dehydrogenase;
DE Short=L-LDH;
DE EC=1.1.1.27;
GN Name=ldh; Synonyms=ldhL;
OS Selenomonas ruminantium.
OC Bacteria; Firmicutes; Negativicutes; Selenomonadales; Selenomonadaceae;
OC Selenomonas.
OX NCBI_TaxID=971;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CATALYTIC ACTIVITY.
RC STRAIN=HD4;
RX PubMed=11821921; DOI=10.1007/s00284-001-0082-9;
RA Evans J.D., Martin S.A.;
RT "Cloning of the L-lactate dehydrogenase gene from the ruminal bacterium
RT Selenomonas ruminantium HD4.";
RL Curr. Microbiol. 44:155-160(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-lactate + NAD(+) = H(+) + NADH + pyruvate;
CC Xref=Rhea:RHEA:23444, ChEBI:CHEBI:15361, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16651, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.27;
CC Evidence={ECO:0000269|PubMed:11821921};
CC -!- PATHWAY: Fermentation; pyruvate fermentation to lactate; (S)-lactate
CC from pyruvate: step 1/1.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family.
CC {ECO:0000305}.
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DR EMBL; AF287913; AAG01001.1; -; Genomic_DNA.
DR RefSeq; WP_014423306.1; NZ_FRBC01000007.1.
DR PDB; 7NAY; X-ray; 1.84 A; A=1-318.
DR PDBsum; 7NAY; -.
DR AlphaFoldDB; Q9EVR0; -.
DR SMR; Q9EVR0; -.
DR STRING; 1392502.JNIO01000007_gene1117; -.
DR eggNOG; COG0039; Bacteria.
DR OMA; AWSHVTI; -.
DR UniPathway; UPA00554; UER00611.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004459; F:L-lactate dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR Gene3D; 3.90.110.10; -; 1.
DR InterPro; IPR001557; L-lactate/malate_DH.
DR InterPro; IPR011304; L-lactate_DH.
DR InterPro; IPR022383; Lactate/malate_DH_C.
DR InterPro; IPR001236; Lactate/malate_DH_N.
DR InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF02866; Ldh_1_C; 1.
DR Pfam; PF00056; Ldh_1_N; 1.
DR PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR PRINTS; PR00086; LLDHDRGNASE.
DR SUPFAM; SSF51735; SSF51735; 1.
DR SUPFAM; SSF56327; SSF56327; 1.
DR TIGRFAMs; TIGR01771; L-LDH-NAD; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; NAD; Oxidoreductase; Phosphoprotein.
FT CHAIN 1..318
FT /note="L-lactate dehydrogenase"
FT /id="PRO_0000168378"
FT ACT_SITE 180
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 13..41
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 93
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 125
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 125
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 156
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 234
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT MOD_RES 225
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250"
FT STRAND 6..10
FT /evidence="ECO:0007829|PDB:7NAY"
FT HELIX 14..25
FT /evidence="ECO:0007829|PDB:7NAY"
FT STRAND 30..35
FT /evidence="ECO:0007829|PDB:7NAY"
FT HELIX 39..52
FT /evidence="ECO:0007829|PDB:7NAY"
FT STRAND 62..65
FT /evidence="ECO:0007829|PDB:7NAY"
FT HELIX 68..71
FT /evidence="ECO:0007829|PDB:7NAY"
FT STRAND 75..79
FT /evidence="ECO:0007829|PDB:7NAY"
FT HELIX 93..96
FT /evidence="ECO:0007829|PDB:7NAY"
FT HELIX 97..114
FT /evidence="ECO:0007829|PDB:7NAY"
FT STRAND 116..122
FT /evidence="ECO:0007829|PDB:7NAY"
FT STRAND 124..126
FT /evidence="ECO:0007829|PDB:7NAY"
FT HELIX 127..136
FT /evidence="ECO:0007829|PDB:7NAY"
FT HELIX 142..144
FT /evidence="ECO:0007829|PDB:7NAY"
FT STRAND 145..147
FT /evidence="ECO:0007829|PDB:7NAY"
FT HELIX 151..165
FT /evidence="ECO:0007829|PDB:7NAY"
FT HELIX 169..171
FT /evidence="ECO:0007829|PDB:7NAY"
FT STRAND 172..178
FT /evidence="ECO:0007829|PDB:7NAY"
FT STRAND 184..193
FT /evidence="ECO:0007829|PDB:7NAY"
FT HELIX 198..200
FT /evidence="ECO:0007829|PDB:7NAY"
FT HELIX 201..204
FT /evidence="ECO:0007829|PDB:7NAY"
FT HELIX 213..231
FT /evidence="ECO:0007829|PDB:7NAY"
FT HELIX 236..250
FT /evidence="ECO:0007829|PDB:7NAY"
FT STRAND 255..265
FT /evidence="ECO:0007829|PDB:7NAY"
FT HELIX 266..268
FT /evidence="ECO:0007829|PDB:7NAY"
FT STRAND 270..281
FT /evidence="ECO:0007829|PDB:7NAY"
FT STRAND 284..288
FT /evidence="ECO:0007829|PDB:7NAY"
FT HELIX 295..314
FT /evidence="ECO:0007829|PDB:7NAY"
SQ SEQUENCE 318 AA; 34975 MW; 04B73C60F3630254 CRC64;
MNNRRKIVVI GASNVGSAVA NKIADFQLAT EVVLIDLNED KAWGEAKDSS HATSCIYSTN
IKFHLGDYED CKDANIIVIT AGPSIRPGET PDRLKLAGTN AKIMSSVMGE IVKRTKEAMI
IMITNPLDVA TYVVSTQFDY PRNLILGTGT MLETYRFRRI LADKYQVDPK NINGYVLGEH
GNAAFVAWST TGCAGFPIDD LDEYFHRTEK LSHEAVEQEL VQVAYDVINK KGFTNTGIAM
AACRFIKSVL YDEHTILPCS AVLEGEYGIK DVALSIPRMV CADGIMRSFE VHLTDDELEK
MHKAAQSVRS ALDGAGIK
