ID   LDRA_ECOLI              Reviewed;          35 AA.
AC   P0DPD0; Q2MBG4; Q6BF86;
DT   20-DEC-2017, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2017, sequence version 1.
DT   03-AUG-2022, entry version 19.
DE   RecName: Full=Small toxic polypeptide LdrA;
GN   Name=ldrA; OrderedLocusNames=b4419, JW5957;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   IDENTIFICATION.
RX   PubMed=12123448; DOI=10.1046/j.1365-2958.2002.03042.x;
RA   Kawano M., Oshima T., Kasai H., Mori H.;
RT   "Molecular characterization of long direct repeat (LDR) sequences
RT   expressing a stable mRNA encoding for a 35-amino-acid cell-killing peptide
RT   and a cis-encoded small antisense RNA in Escherichia coli.";
RL   Mol. Microbiol. 45:333-349(2002).
RN   [4]
RP   FUNCTION AS A TOXIN, AND SUBCELLULAR LOCATION.
RX   PubMed=24513967; DOI=10.1159/000357949;
RA   Yamaguchi Y., Tokunaga N., Inouye M., Phadtare S.;
RT   "Characterization of LdrA (long direct repeat A) protein of Escherichia
RT   coli.";
RL   J. Mol. Microbiol. Biotechnol. 24:91-97(2014).
CC   -!- FUNCTION: Toxic component of a type I toxin-antitoxin (TA) system.
CC       Inhibits ATP synthesis possibly due to its insertion in the cell inner
CC       membrane, ATP levels drop over 50% 2 minutes after induction
CC       (PubMed:24513967). Overexpression is toxic leading to cell death, it
CC       inhibits cell growth within 30 minutes; C-terminally tagged versions of
CC       the protein are toxic while N-terminally tagged versions are not
CC       (PubMed:24513967). {ECO:0000269|PubMed:24513967}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000269|PubMed:24513967}; Single-pass membrane protein
CC       {ECO:0000305|PubMed:24513967}.
CC   -!- INDUCTION: Expression of the proteinaceous toxin is probably controlled
CC       by an antisense sRNA, in this case RdlA. Only a few of these TA systems
CC       have been mechanistically characterized; the mechanisms used to control
CC       expression of the toxin gene are not necessarily the same (Probable).
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Ldr toxic peptide family. {ECO:0000305}.
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DR   EMBL; U00096; AAT48126.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76392.1; -; Genomic_DNA.
DR   RefSeq; WP_000170955.1; NZ_SSZK01000010.1.
DR   RefSeq; YP_025297.1; NC_000913.3.
DR   RefSeq; YP_025299.1; NC_000913.3.
DR   AlphaFoldDB; P0DPD0; -.
DR   SMR; P0DPD0; -.
DR   EnsemblBacteria; AAT48126; AAT48126; b4419.
DR   EnsemblBacteria; BAE76392; BAE76392; BAE76392.
DR   GeneID; 2847733; -.
DR   GeneID; 2847775; -.
DR   GeneID; 58460425; -.
DR   KEGG; ecj:JW5957; -.
DR   KEGG; eco:b4419; -.
DR   KEGG; eco:b4423; -.
DR   HOGENOM; CLU_212598_1_0_6; -.
DR   BioCyc; EcoCyc:MON0-1601; -.
DR   PRO; PR:P0DPD0; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR   GO; GO:0008219; P:cell death; IMP:EcoCyc.
DR   InterPro; IPR025253; Toxin_Ldr.
DR   Pfam; PF13940; Ldr_toxin; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Toxin-antitoxin system; Transmembrane; Transmembrane helix.
FT   PEPTIDE         1..35
FT                   /note="Small toxic polypeptide LdrA"
FT                   /id="PRO_0000230292"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   35 AA;  4014 MW;  5C87B0B2BC271596 CRC64;
     MTLAQFAMIF WHDLAAPILA GIITAAIVSW WRNRK