ID   LDRD_ECOLI              Reviewed;          35 AA.
AC   Q6BF25; Q2M7K0;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Small toxic polypeptide LdrD;
GN   Name=ldrD; OrderedLocusNames=b4453, JW5966;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   IDENTIFICATION, FUNCTION, AND PROBABLE INDUCTION.
RX   PubMed=12123448; DOI=10.1046/j.1365-2958.2002.03042.x;
RA   Kawano M., Oshima T., Kasai H., Mori H.;
RT   "Molecular characterization of long direct repeat (LDR) sequences
RT   expressing a stable mRNA encoding for a 35-amino-acid cell-killing peptide
RT   and a cis-encoded small antisense RNA in Escherichia coli.";
RL   Mol. Microbiol. 45:333-349(2002).
RN   [4]
RP   FUNCTION.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=18710431; DOI=10.1111/j.1365-2958.2008.06394.x;
RA   Fozo E.M., Kawano M., Fontaine F., Kaya Y., Mendieta K.S., Jones K.L.,
RA   Ocampo A., Rudd K.E., Storz G.;
RT   "Repression of small toxic protein synthesis by the Sib and OhsC small
RT   RNAs.";
RL   Mol. Microbiol. 70:1076-1093(2008).
CC   -!- FUNCTION: Toxic component of a type I toxin-antitoxin (TA) system.
CC       Overexpression causes rapid cell killing and nucleoid condensation of
CC       the host cell (PubMed:12123448). Overexpression induces stress-response
CC       and a number of membrane protein genes. May inhibit ATP synthesis due
CC       to its insertion in the cell inner membrane (By similarity).
CC       {ECO:0000250|UniProtKB:P0DPD0, ECO:0000269|PubMed:12123448,
CC       ECO:0000269|PubMed:18710431}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255}; Single-pass
CC       membrane protein {ECO:0000255}.
CC   -!- INDUCTION: Expression of the proteinaceous toxin is controlled by an
CC       antisense sRNA, in this case RdlD. Only a few of these TA systems have
CC       been mechanistically characterized; the mechanisms used to control
CC       expression of the toxin gene are not necessarily the same (Probable).
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Ldr toxic peptide family. {ECO:0000305}.
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DR   EMBL; U00096; AAT48189.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77756.1; -; Genomic_DNA.
DR   RefSeq; WP_000141634.1; NZ_SSZK01000039.1.
DR   RefSeq; YP_026227.1; NC_000913.3.
DR   PDB; 5LBJ; NMR; -; A=1-35.
DR   PDBsum; 5LBJ; -.
DR   AlphaFoldDB; Q6BF25; -.
DR   SMR; Q6BF25; -.
DR   BioGRID; 4260912; 12.
DR   STRING; 511145.b4453; -.
DR   TCDB; 1.C.64.1.13; the fst toxin (fst) family.
DR   PaxDb; Q6BF25; -.
DR   PRIDE; Q6BF25; -.
DR   EnsemblBacteria; AAT48189; AAT48189; b4453.
DR   EnsemblBacteria; BAE77756; BAE77756; BAE77756.
DR   GeneID; 2847730; -.
DR   GeneID; 58390103; -.
DR   KEGG; ecj:JW5966; -.
DR   KEGG; eco:b4453; -.
DR   PATRIC; fig|1411691.4.peg.3177; -.
DR   HOGENOM; CLU_212598_1_0_6; -.
DR   PhylomeDB; Q6BF25; -.
DR   BioCyc; EcoCyc:MON0-921; -.
DR   PRO; PR:Q6BF25; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008219; P:cell death; IMP:EcoCyc.
DR   InterPro; IPR025253; Toxin_Ldr.
DR   Pfam; PF13940; Ldr_toxin; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell inner membrane; Cell membrane; Membrane;
KW   Reference proteome; Toxin-antitoxin system; Transmembrane;
KW   Transmembrane helix.
FT   PEPTIDE         1..35
FT                   /note="Small toxic polypeptide LdrD"
FT                   /id="PRO_0000230294"
FT   TRANSMEM        10..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   HELIX           3..34
FT                   /evidence="ECO:0007829|PDB:5LBJ"
SQ   SEQUENCE   35 AA;  3917 MW;  25E6A9A8634CD78D CRC64;
     MTFAELGMAF WHDLAAPVIA GILASMIVNW LNKRK