LDRP_THET8
ID LDRP_THET8 Reviewed; 195 AA.
AC Q53W63;
DT 11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=Transcriptional regulator LdrP {ECO:0000303|PubMed:22383301, ECO:0000312|EMBL:BAD71895.1};
DE AltName: Full=Cyclic AMP receptor protein/Fumarate and nitrate reduction regulator superfamily protein LdrP {ECO:0000303|PubMed:22383301};
DE Short=CRP/FNR superfamily protein LdrP {ECO:0000303|PubMed:22383301};
DE AltName: Full=Light induced transcription regulator-dependent regulatory protein {ECO:0000305};
DE AltName: Full=LitR-dependent regulatory protein {ECO:0000305};
DE Short=LdrP {ECO:0000305};
GN OrderedLocusNames=TTHB099 {ECO:0000312|EMBL:BAD71895.1};
OS Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OG Plasmid pTT27 {ECO:0000312|EMBL:BAD71895.1,
OG ECO:0000312|Proteomes:UP000000532}.
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=300852 {ECO:0000312|EMBL:BAD71895.1};
RN [1] {ECO:0000312|EMBL:BAD71895.1, ECO:0000312|Proteomes:UP000000532}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8 {ECO:0000312|Proteomes:UP000000532};
RA Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT "Complete genome sequence of Thermus thermophilus HB8.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0007744|PDB:3B02}
RP X-RAY CRYSTALLOGRAPHY (1.92 ANGSTROMS), AND SUBUNIT.
RC STRAIN=ATCC 27634 / DSM 579 / HB8 {ECO:0000303|PubMed:22383301};
RX PubMed=22383301; DOI=10.1002/prot.24049;
RA Agari Y., Kuramitsu S., Shinkai A.;
RT "X-ray crystal structure of TTHB099, a CRP/FNR superfamily transcriptional
RT regulator from Thermus thermophilus HB8, reveals a DNA-binding protein with
RT no required allosteric effector molecule.";
RL Proteins 80:1490-1494(2012).
CC -!- FUNCTION: Activates transcription. Positively regulates PcrtB promoter
CC upstream of the crtB operon in a cAMP-independent manner. Regulated
CC genes include genes encoding DNA photolyase, phytoene synthase and
CC cytochrome P450 monooxygenase, which are involved in carotenoid
CC biosynthesis. Positively regulates the light-inducible gene cluster in
CC the megaplasmid in a cAMP-independent manner.
CC {ECO:0000250|UniProtKB:Q746J8}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:22383301}.
CC -!- INTERACTION:
CC Q53W63; Q5SHR6: rpoA; NbExp=2; IntAct=EBI-16208148, EBI-2106870;
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DR EMBL; AP008227; BAD71895.1; -; Genomic_DNA.
DR RefSeq; WP_011229195.1; NC_006462.1.
DR RefSeq; YP_145338.1; NC_006462.1.
DR PDB; 3B02; X-ray; 1.92 A; A=1-195.
DR PDB; 5I2D; X-ray; 4.41 A; G/H/R/S=1-195.
DR PDBsum; 3B02; -.
DR PDBsum; 5I2D; -.
DR AlphaFoldDB; Q53W63; -.
DR SMR; Q53W63; -.
DR DIP; DIP-62042N; -.
DR IntAct; Q53W63; 5.
DR EnsemblBacteria; BAD71895; BAD71895; BAD71895.
DR GeneID; 3169578; -.
DR KEGG; ttj:TTHB099; -.
DR PATRIC; fig|300852.9.peg.2042; -.
DR HOGENOM; CLU_075053_3_1_0; -.
DR OMA; PRAMDHE; -.
DR PhylomeDB; Q53W63; -.
DR EvolutionaryTrace; Q53W63; -.
DR Proteomes; UP000000532; Plasmid pTT27.
DR GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR CDD; cd00038; CAP_ED; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 2.60.120.10; -; 1.
DR InterPro; IPR018490; cNMP-bd-like.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR012318; HTH_CRP.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00027; cNMP_binding; 1.
DR Pfam; PF13545; HTH_Crp_2; 1.
DR SMART; SM00100; cNMP; 1.
DR SMART; SM00419; HTH_CRP; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF51206; SSF51206; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
DR PROSITE; PS51063; HTH_CRP_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Plasmid; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..195
FT /note="Transcriptional regulator LdrP"
FT /id="PRO_0000436261"
FT DOMAIN 110..182
FT /note="HTH crp-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
FT DNA_BIND 142..161
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00387"
FT STRAND 2..4
FT /evidence="ECO:0007829|PDB:3B02"
FT STRAND 9..11
FT /evidence="ECO:0007829|PDB:3B02"
FT STRAND 20..26
FT /evidence="ECO:0007829|PDB:3B02"
FT STRAND 28..33
FT /evidence="ECO:0007829|PDB:3B02"
FT STRAND 39..45
FT /evidence="ECO:0007829|PDB:3B02"
FT HELIX 53..56
FT /evidence="ECO:0007829|PDB:3B02"
FT STRAND 62..76
FT /evidence="ECO:0007829|PDB:3B02"
FT HELIX 78..80
FT /evidence="ECO:0007829|PDB:3B02"
FT HELIX 83..107
FT /evidence="ECO:0007829|PDB:3B02"
FT HELIX 112..123
FT /evidence="ECO:0007829|PDB:3B02"
FT STRAND 129..132
FT /evidence="ECO:0007829|PDB:3B02"
FT STRAND 135..139
FT /evidence="ECO:0007829|PDB:3B02"
FT HELIX 142..147
FT /evidence="ECO:0007829|PDB:3B02"
FT TURN 148..150
FT /evidence="ECO:0007829|PDB:3B02"
FT HELIX 153..165
FT /evidence="ECO:0007829|PDB:3B02"
FT STRAND 168..172
FT /evidence="ECO:0007829|PDB:3B02"
FT STRAND 175..178
FT /evidence="ECO:0007829|PDB:3B02"
FT HELIX 181..188
FT /evidence="ECO:0007829|PDB:3B02"
SQ SEQUENCE 195 AA; 22138 MW; 90A80BA5CB186A76 CRC64;
MKRFARKETI YLRGEEARTL YRLEEGLVRV VELLPDGRLI TLRHVLPGDY FGEEALEGKA
YRYTAEAMTE AVVQGLEPRA MDHEALHRVA RNLARQMRRV QAYEAHLQTG ELRARIARYL
LFLADTPLSA RDRQGIYVTV SHEEIADATA SIRESVSKVL ADLRREGLIA TAYRRVYLLD
LAALEREAGS ALEAA
