ARE1_SACU7
ID ARE1_SACU7 Reviewed; 623 AA.
AC Q876L3;
DT 09-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 60.
DE RecName: Full=Sterol O-acyltransferase 1;
DE EC=2.3.1.-;
DE AltName: Full=Sterol-ester synthase 1;
GN Name=ARE1;
OS Saccharomyces uvarum (strain ATCC 76518 / CBS 7001 / CLIB 283 / NBRC 10550
OS / MCYC 623 / NCYC 2669 / NRRL Y-11845) (Yeast) (Saccharomyces bayanus var.
OS uvarum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=659244;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=623-6C / CBS 9787 / CLIB 533;
RX PubMed=12594514; DOI=10.1038/nature01419;
RA Langkjaer R.B., Cliften P.F., Johnston M., Piskur J.;
RT "Yeast genome duplication was followed by asynchronous differentiation of
RT duplicated genes.";
RL Nature 421:848-852(2003).
CC -!- FUNCTION: Sterol O-acyltransferase that catalyzes the formation of
CC stery esters. {ECO:0000250|UniProtKB:P25628}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:P25628}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the membrane-bound acyltransferase family.
CC Sterol o-acyltransferase subfamily. {ECO:0000305}.
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DR EMBL; AY144798; AAO32362.1; -; Genomic_DNA.
DR AlphaFoldDB; Q876L3; -.
DR SMR; Q876L3; -.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008374; F:O-acyltransferase activity; IEA:InterPro.
DR InterPro; IPR004299; MBOAT_fam.
DR InterPro; IPR014371; Oat_ACAT_DAG_ARE.
DR PANTHER; PTHR10408; PTHR10408; 1.
DR Pfam; PF03062; MBOAT; 1.
DR PIRSF; PIRSF000439; Oat_ACAT_DAG_ARE; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Endoplasmic reticulum; Membrane; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..623
FT /note="Sterol O-acyltransferase 1"
FT /id="PRO_0000207647"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 242..262
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 277..297
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 384..404
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 422..442
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 548..568
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 603..623
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 20..99
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 504..510
FT /note="FYXDWWN motif"
FT /evidence="ECO:0000250|UniProtKB:P35610"
FT COMPBIAS 23..59
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 560
FT /evidence="ECO:0000250|UniProtKB:P35610"
SQ SEQUENCE 623 AA; 71794 MW; 86635C60C4741A35 CRC64;
MTEAKELLQD ERFLKIQELN SAEPSKRHSV TYDNVILPQE SVEVSPRSST TSLEEPATTT
ATGVAVAAAA AAAEKKKKKK GEDGDDEQDE QAEEKYPVDP RMQKYLSHLK SKSRTRVHRK
DASKYVSFFG DVSFDPRPTL LDSAVNVPFQ TTFKGPVLEK QLKGLQQTKK GEVAAAAATA
ATAATAAAAP PGKKLESNFS GIYVFAWMFM GWIAFRSCMD YYVSHEGGFA SMEIVQYMTS
DLFTIALLDL ALFLSTFFVV FVHWLVKLGF IRWKWTGFVA VSLFELCFIP VSFPVYVYYF
HFSWVTRIFL FLHSVVLLMK AHSFAFYNGY LWDIKNELEF SSNKLNKFKE SLSPETKDIL
QKSCDFCLFE LNYQTKDNDF PNNISCSNYF MFCMFPVLVY QINYPRTSHI RWRYVLEKFC
AIMGTIFLMM VTAQIFMHPV AMRCIEYHDT PSFGGWVPAV KQWLFLLFEM IPGFSVLYML
TFYMIWDALL NCVAELTRFA DRYFYGDWWN CVSFEEFSRI WNVPVHKFLL RHVYHSSMGA
LHFSKAQATL FTFLLSAVFH EIAMFAIFKE VRGYLFLFQL SQFAWTALSN TKFLRSRPQL
SNVVFTFGVC TGPSMIMTLY LTL