LEA18_ARATH
ID LEA18_ARATH Reviewed; 97 AA.
AC Q96273;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Late embryogenesis abundant protein 18 {ECO:0000303|PubMed:18318901};
DE AltName: Full=Late embryogenesis abundant protein 4-2 {ECO:0000303|PubMed:20668063};
DE Short=AtLEA4-2 {ECO:0000303|PubMed:20668063};
GN Name=LEA18 {ECO:0000303|PubMed:18318901};
GN Synonyms=LEA4-2 {ECO:0000303|PubMed:20668063};
GN OrderedLocusNames=At2g35300 {ECO:0000312|Araport:AT2G35300};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia; TISSUE=Dry seed;
RA Raynal M.;
RT "A.thaliana mRNA for LEA D113 type 2 protein.";
RL Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18318901; DOI=10.1186/1471-2164-9-118;
RA Hundertmark M., Hincha D.K.;
RT "LEA (late embryogenesis abundant) proteins and their encoding genes in
RT Arabidopsis thaliana.";
RL BMC Genomics 9:118-118(2008).
RN [6]
RP FUNCTION, DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=20668063; DOI=10.1104/pp.110.158964;
RA Olvera-Carrillo Y., Campos F., Reyes J.L., Garciarrubio A.,
RA Covarrubias A.A.;
RT "Functional analysis of the group 4 late embryogenesis abundant proteins
RT reveals their relevance in the adaptive response during water deficit in
RT Arabidopsis.";
RL Plant Physiol. 154:373-390(2010).
RN [7]
RP FUNCTION.
RX PubMed=20875393; DOI=10.1016/j.bbamem.2010.09.010;
RA Hundertmark M., Dimova R., Lengefeld J., Seckler R., Hincha D.K.;
RT "The intrinsically disordered late embryogenesis abundant protein LEA18
RT from Arabidopsis thaliana modulates membrane stability through binding and
RT folding.";
RL Biochim. Biophys. Acta 1808:446-453(2011).
RN [8]
RP FUNCTION.
RX PubMed=27006402; DOI=10.1074/jbc.m116.720318;
RA Cuevas-Velazquez C.L., Saab-Rincon G., Reyes J.L., Covarrubias A.A.;
RT "The unstructured N-terminal region of Arabidopsis group 4 late
RT embryogenesis abundant (LEA) proteins is required for folding and for
RT chaperone-like activity under water deficit.";
RL J. Biol. Chem. 291:10893-10903(2016).
CC -!- FUNCTION: Involved dehydration tolerance. Involved in the adaptive
CC response of vascular plants to withstand water deficit
CC (PubMed:20668063). May possess chaperone-like activity under water
CC deficit (PubMed:27006402). Binds to negatively charged membranes of
CC liposomes. This binding induces partial folding of the largely
CC unstructured LEA18 protein, vesicle aggregation, and leakage of soluble
CC content from liposomes (PubMed:20875393). {ECO:0000269|PubMed:20668063,
CC ECO:0000269|PubMed:20875393, ECO:0000269|PubMed:27006402}.
CC -!- DEVELOPMENTAL STAGE: Expressed during embryo development and in dry
CC seed. Expression decreases significantly after seed germination.
CC {ECO:0000269|PubMed:20668063}.
CC -!- INDUCTION: By dehydration stress. {ECO:0000269|PubMed:20668063}.
CC -!- MISCELLANEOUS: Under water deficit, the N-terminal region is necessary
CC and sufficient for conformational transition from disordered to alpha-
CC helix folding. This conformational transition is required for
CC chaperone-like activity under water limitation.
CC {ECO:0000269|PubMed:27006402}.
CC -!- SIMILARITY: Belongs to the LEA type 1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X91917; CAA63010.1; -; mRNA.
DR EMBL; AC004667; AAC61808.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09091.1; -; Genomic_DNA.
DR EMBL; AY072383; AAL62375.1; -; mRNA.
DR EMBL; AY114622; AAM47941.1; -; mRNA.
DR PIR; H84766; H84766.
DR RefSeq; NP_181073.1; NM_129082.4.
DR AlphaFoldDB; Q96273; -.
DR STRING; 3702.AT2G35300.1; -.
DR PaxDb; Q96273; -.
DR PRIDE; Q96273; -.
DR ProteomicsDB; 230392; -.
DR DNASU; 818097; -.
DR EnsemblPlants; AT2G35300.1; AT2G35300.1; AT2G35300.
DR GeneID; 818097; -.
DR Gramene; AT2G35300.1; AT2G35300.1; AT2G35300.
DR KEGG; ath:AT2G35300; -.
DR Araport; AT2G35300; -.
DR TAIR; locus:2063409; AT2G35300.
DR eggNOG; ENOG502SG65; Eukaryota.
DR HOGENOM; CLU_2336558_0_0_1; -.
DR InParanoid; Q96273; -.
DR OMA; KMQGKTG; -.
DR OrthoDB; 1639513at2759; -.
DR PhylomeDB; Q96273; -.
DR PRO; PR:Q96273; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q96273; baseline and differential.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IEA:InterPro.
DR GO; GO:0048868; P:pollen tube development; IMP:TAIR.
DR GO; GO:0006970; P:response to osmotic stress; IMP:TAIR.
DR GO; GO:0009414; P:response to water deprivation; IMP:TAIR.
DR GO; GO:0048316; P:seed development; IMP:TAIR.
DR DisProt; DP01299; -.
DR InterPro; IPR005513; LEA_1.
DR PANTHER; PTHR33493; PTHR33493; 1.
DR Pfam; PF03760; LEA_1; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Reference proteome; Stress response.
FT CHAIN 1..97
FT /note="Late embryogenesis abundant protein 18"
FT /id="PRO_0000438097"
FT REGION 1..97
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 34..63
FT /evidence="ECO:0000255"
FT COMPBIAS 31..73
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 97 AA; 10481 MW; AE983626A3CE22BD CRC64;
MQSAKEKISD MASTAKEKLN IGGAKAQGHA EKTMARTKKE KKLAQEREKS KEAQAKADLH
QSKAEHAADA QVHGHHLPGH STYPTRATGA NYPPGQI
